Nematode resistant crops

ABSTRACT

Methods of inhibiting plant parasitic nematodes, methods of obtaining transgenic plants useful for inhibiting such nematodes, and transgenic plants that are resistant to plant parasitic nematodes through inhibition of plant nematode CLAVATA3/ESR (CLE) peptide receptor genes are provided. Methods for expressing genes at plant parasitic nematode feeding sites with plant nematode CLE peptide receptor gene promoters are also provided, along with nematode CLE peptide receptor gene promoters that are useful for expressing genes in nematode feeding sites as well as transgenic plants and nematode resistant transgenic plants comprising the promoters.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a National Stage application of International Patent Application No PCT/US2011/043882, filed Jul. 13, 2011 and incorporated herein by reference in its entirety, and claims the benefit under 35 USC § 119(e) of U.S. Provisional Application Ser. No, 61/371,619, filed Aug 6, 2010, which is incorporated herein by reference in its entirety.

STATEMENT REGARDING GOVERNMENTAL SUPPORT

This invention was made with Government support under Grant Numbers 2007-35607-17790, 2008-34113-19420, 2009-35302-05304, 2005-35604-15434, and 2008-35302-18824, all awarded by the USDA-NRI. The government has certain rights to this invention.

INCORPORATION OF SEQUENCE LISTING

The sequence listing that is contained in the file named “52553_111240_ST25.txt ”, which is 312,382 bytes in size (measured in operating system MS-Windows), created on Feb. 4, 2013, is filed herewith by electronic submission and incorporated herein by reference in its entirety.

BACKGROUND

Obligate biotrophs are pathogens that establish intimate parasitic relationships with the host that they infect. Often times these relationships involve some kind of modification or reprogramming of the host cell(s) to accommodate the pathogen's subsequent growth and development. Plant-parasitic nematodes are obligate biotrophs that mainly attack the roots of plants and cause over $100 billion in crop damage annually (Sasser and Freckman, 1987). The most economically important plant-parasitic nematodes include the cyst forming nematodes of Heterodera and Globodera spp. These sedentary endoparasitic nematodes form intimate parasitic relationships with their hosts by penetrating the root as motile juveniles and migrating intracellularly until they reach the root vasculature where they select a single cell to initiate a feeding site. The initial syncytial cell undergoes developmental changes to re-differentiate into a syncytium to support subsequent nematode growth and development in later sedentary stages (Davis et al., 2004). The syncytium forms when neighboring cells fuse as a result of partial cell wall degradation (Endo, 1964), creating a permanent feeding cell that shares characteristics with plant cell types including meristematic cells, endosperm cells, transfer cells, and developing xylem (Mitchum et al., 2008). It has been proposed that the development and maintenance of the syncytium is dependent on the secretory effector proteins originating in the esophageal gland cells and delivered into the host root through the stylet of plant-parasitic nematodes (Davis et al., 2008). Recently, the cyst nematode secreted CLAVATA3/ESR(CLE)-like effector proteins have been shown to act as ligand mimics of plant CLE peptides, and are required for successful nematode infection (Wang et al., 2005; Patel et al., 2008; Lu et al., 2009; Wang et al., 2010a; Wang et al., 2010b).

Plant CLEs are small peptide ligands involved in regulating a population of specialized cells, called stem cells, which allow postembryonic organogenesis to occur (Simon and Stahl 2006). These stem cell pools can be found in the shoot apical meristem (SAM), the root apical meristem (RAM), and the vascular cambium. Whether or not these stems cells remain in an undifferentiated state or differentiate into new plant tissues is tightly controlled by CLE signaling pathways. In Arabidopsis, the population of stem cells which resides in the organizing center (OC) of the SAM is maintained by the expression of the transcription factor WUSCHEL (WUS) (Laux et al., 1996). Differentiation of those stems cells is promoted when the ligand-receptor pair of CLAVATA3 (CLV3), a small extracellular peptide ligand in the CLE family (Fletcher et al., 1999; Rojo et al., 2002), binds to CLV1 (Ogawa et al., 2008), a leucine-rich-repeat receptor like kinase (LRR-RLK) and downregulates WUS. Previous models have suggested that CLV1 forms a receptor complex with the LRR-receptor like protein (RLP) CLV2 (Clark et al., 1993; Kayes and Clark, 1998; Jeong et al., 1999; Trotochaud et al., 1999). More recently, it has been suggested that CLV1 acts in parallel or together with the heterodimer receptor complex of CLV2 and CORYNE (CRN) (Miwa et al., 2008; Muller, 2008; Bleckmann et al., 2010; Zhu et al., 2010). In comparison to the SAM, much less is known about the regulation of the stem cells in the RAM. The quiescent center (QC) is the equivalent to the OC in the SAM. However, there are significant differences between the OC and the QC. In contrast to the OC, the cells surrounding the QC are maintained as stem cells. In addition, stem cells are differentiated in both proximal and distal directions. This indicates that there is a signaling ligand involved in cell-cell communication to maintain the cells surrounding the QC as stem cells, and a signal to promote differentiation (Sarkar et al., 2007; Stahl et al., 2009). Previous reports have identified that the WUS-related homeobox 5 (WOX5) transcription factor is expressed in the QC of the RAM and is required to maintain the distal stem cell pool (Sarkar et al., 2007). Recently it has been shown that CLE40, the closest homolog to CLV3, is expressed in the columella cells and regulates expression of WOX5 (Stahl et al., 2009). The WOX5/CLE40 signaling pathway appears to only control the distal stem cell pool, indicating that other CLE signaling pathways may exist to control the proximal stem cell pool. Consistent with these observations, a number of Arabidopsis CLEs are expressed in roots (Sharma et al., 2003), and when some of these CLEs are overexpressed they have been shown to cause premature termination of the primary root meristem (Fiers et al., 2004; Strabala et al., 2006; Meng et al., 2010). In addition, the short root phenotype has been shown to be dependent on CLV2 and CRN perception (Casamitjana-Martinez et al., 2003; Fiers et al., 2005; Miwa et al., 2008; Meng et al., 2010). Taken together this indicates that a CLV-like and CLE-controlled signaling pathway can act in the root.

CLE-like genes from nematodes have been reported in the soybean cyst nematode (SCN, H. glycines) (Wang et al., 2005; Wang et al., 2010a), the beet cyst nematode (BCN, H. schachtii) (Patel et al., 2008; Wang et al., 2010b), and the potato cyst nematode (PCN, G. rostochiensis) (Lu et al., 2009). BCN CLEs have been detected in the dorsal gland ampulla indicating they are likely secreted from the stylet into host cells (Patel et al., 2008). More recently, SCN CLEs have been shown to be secreted directly to the syncytial cytoplasm where the variable domain is thought to redirect the nematode CLE peptides to the apoplast (Wang et al., 2010a). These findings suggest that when delivered to the apoplast, nematode CLEs would be available to interact with extracellular receptors to function as ligand mimics of plant CLE signaling pathways. Overexpression studies have shown that nematode CLEs can trigger plant CLE signaling pathways (Wang et al., 2005; Lu et al., 2009; Wang et al., 2010a; Wang et al., 2010b), but the identity of the receptors and downstream signaling pathways that are activated to initiate developmental cascades required for the re-differentiation of root cells to form syncytia, are currently unknown.

US Patent Applications 20090077687 and 20090012029, identified nematode parasitism (effector) genes and described potential mechanisms to disrupt their expression and the function of their products to inhibit nematode parasitism of plants.

SUMMARY OF INVENTION

This invention provides for methods of inhibiting plant parasitic nematodes, methods of obtaining transgenic plants useful for inhibiting such nematodes, methods for expressing genes at plant parasitic nematode feeding sites, and transgenic plants that are resistant to plant parasitic nematodes. Also provided are promoters including, but not limited to a BAM1 promoter, that are useful for expressing genes in nematode feeding sites as well as transgenic plants and nematode resistant transgenic plants comprising the same. It is anticipated that the BAM1 and other promoters provided herewith can in certain embodiments be operably linked to genes that provide for inhibition of plant parasitic nematodes when introduced into transgenic plants and for plants that display such inhibition. Such genes that provide for inhibition of plant parasitic nematodes that can be used with the promoters provided herewith are disclosed in US Patent Application 20090012029, which is specifically incorporated herein by reference in its entirety.

In certain embodiments, a method for inhibiting plant parasitic nematode damage to a plant comprising growing a plant comprising a mutation or a transgene that provides for inhibition of at least one endogenous plant gene encoding a receptor for a nematode CLE peptide in the presence of plant parasitic nematodes is provided. In certain embodiments of these methods, the plant gene encoding a receptor for a nematode CLE peptide is selected from the group consisting of a CLV1-like gene, a CLAVATA2-like (CLV2-like) gene, a BAM1-like gene, a BAM2-like gene, a CRN-like gene, a ACR4-like gene, an ER-like gene, and an ERL2-like gene. In certain embodiments of these methods, CLV1-like gene, said CLV2-like gene, BAM1-like gene, a BAM2-like gene, a CRN-like gene, a ACR4-like gene, an ER-like gene, or an ERL2-like gene is an ortholog of a corresponding Arabidopsis, soybean, or potato CLV1, CLV2, BAM1, BAM2, CRN, ACR4, ER, or ERL2 gene. In certain embodiments of these methods, the methods can further comprise the step of harvesting a product of said plant. In certain embodiments of these methods, the harvested product is a leaf, stem, flower, seed, root, or tuber. In certain embodiments of these methods, the yield and/or quality of said product is increased relative to a control plant that is grown in presence of plant parasitic nematodes and that lacks said mutation or said transgene that provides for inhibition of at least one endogenous plant gene encoding a receptor for a nematode CLE peptide. In certain embodiments of these methods, the transgene comprises: i) an siRNA directed against said plant gene; ii) an artificial microRNA targeting said plant gene; iii) a dominant negative form of said plant gene; iv) an antisense or sense form of said plant gene; or v) a genomic insertion that disrupts said plant gene.

In certain embodiments, a method for obtaining a transgenic plant that exhibits resistance to a plant parasitic nematode comprising the steps of: a) introducing a transgene that provides for inhibition of at least one endogenous plant gene encoding a receptor for a nematode CLE peptide into a plant cell or a transgene that provides for inhibition of at least one CLV1-like, a CLV2-like, a BAM1-like, a BAM2-like, a CRN-like, a ACR4-like, an ER-like, and/or an ERL2-like gene; and b) selecting a transgenic plant obtained from said plant cell, wherein said selected transgenic plant comprises said transgene and exhibits resistance to a plant nematode is provided. In certain embodiments of these methods, CLV1-like gene, said CLV2-like gene, BAM1-like gene, a BAM2-like gene, a CRN-like gene, a ACR4-like gene, an ER-like gene, or an ERL2-like gene is an ortholog of a corresponding Arabidopsis, soybean, or potato CLV1, CLV2, BAM1, BAM2, CRN, ACR4, ER, or ERL2 gene.

In certain embodiments, a method for obtaining a transgenic plant expressing a gene product at a plant parasitic nematode feeding site, comprising the steps of: a) introducing a transgene wherein a CRN, CLV, or BAM promoter is operably linked to a gene encoding said gene product into a plant cell; and, b) selecting a transgenic plant obtained from said plant cell, wherein said selected transgenic plant comprises said transgene and exhibits expression of said gene product at said nematode feeding site is provided. In certain embodiments of these methods, the gene product is inhibitory to the plant parasitic nematode. In certain embodiments of these methods, the inhibitory gene product is a siRNA directed against a plant parasitic nematode gene. In certain embodiments of these aforementioned methods, an ACR4, BAM1, BAM2, CLV1, CLV2, CRN, ER, or ERL2 promoter is operably linked to a gene encoding the gene product. In certain embodiments of these aforementioned methods, an ACR4, BAM1, BAM2, CLV1, CLV2, CRN, ER, or ERL2 promoter is operably linked to a gene product that is inhibitory to a plant parasitic nematode. In certain embodiments of these methods, the inhibitory gene product is an amiRNA directed against a plant parasitic nematode gene.

In certain embodiments of any of the aforementioned methods of inhibiting plant parasitic nematode damage, obtaining a transgenic plant that exhibits resistance to a plant parasitic nematode, or obtaining a transgenic plant expressing a gene product at a plant parasitic nematode feeding site, the plant nematode is a cyst nematode. In certain embodiments of these methods, the cyst nematode is a Heterodera or Globodera spp. In certain embodiments of these methods, the Heterodera spp. is H. avenae, H. bifenestra, H cajani. H. carotae, H. ciceri, H. cruciferae, H. cynodontis, H. cyperi, H. davert, H. elachista, H. fii, H. galeopsidis, H. goettingiana, H. graminis, H. hordecalis, H. humuli, H. iri, H. latipons, H. lespedeza, H. leucilyma, H. Iongicaudata, H. mani, H. maydis, H. medicaginis, H. oryzae, H. oryzicola, H. sacchari, H. salixophila, H. schachtii, H. sorghii, H. trifoii, H. urticae, H. vigna, or H. zeae. In certain embodiments of these methods, the Globodera spp. is G. achilleae, G. artemisiae, G. hypolysi, G. leptonepia, G. mali, G. pallida, G. rostochiensis, G. tabacum, or G. zeylandica.

In certain embodiments of any of the aforementioned methods of inhibiting plant parasitic nematode damage, obtaining a transgenic plant that exhibits resistance to a plant parasitic nematode, or obtaining a transgenic plant expressing a gene product at a plant parasitic nematode feeding site, the plant is a monocot or dicot plant, or is selected from the group consisting of a tobacco, cereal, sugar beet, cotton, fruit, fiber, oilseed, potato, rice, corn, soybean, vegetable, and wheat plant.

In certain embodiments of any of the aforementioned methods of inhibiting plant parasitic nematode damage or obtaining a transgenic plant that exhibits resistance to a plant parasitic nematode, the endogenous plant gene encoding a receptor for a nematode CLE is a potato StCLV1, StCLV2, StBAM1, StBAM2, StCRN, StACR4, StER, or StERL2 gene and the plant is a potato plant. In certain embodiments of these methods, the plant parasitic nematode is G. rostochiensis or G. pallida.

In certain embodiments of any of the aforementioned methods of inhibiting plant parasitic nematode damage or obtaining a transgenic plant that exhibits resistance to a plant parasitic nematode, the endogenous plant gene encoding a receptor for a nematode CLE is selected from the group consisting of soybean genes provided in Table 3 of Example 2 and said plant is a soybean plant. In certain embodiments of any of the aforementioned methods of inhibiting plant parasitic nematode damage, the plant parasitic nematode is Heterodera glycines or H. schachtii.

In certain embodiments, a plant parasitic nematode resistant transgenic plant comprising a transgene that provides for inhibition of at least one endogenous plant gene encoding a receptor for a nematode CLE peptide is provided. In certain embodiments, the transgene comprises: i) an siRNA directed against said plant gene; ii) an artificial microRNA targeting said plant gene; iii) a dominant negative form of said plant gene; iv) an antisense or sense form of said plant gene; or v) a genomic insertion that disrupts said plant gene. In certain embodiments of any of the aforementioned transgenic plants, the endogenous plant gene encoding a receptor for a nematode CLE is selected from the group consisting of soybean genes of provided in Table 3 of Example 2 and the plant is a soybean plant. In certain embodiments of any of the aforementioned transgenic plants, the endogenous plant gene encoding a receptor for a nematode CLE is a potato StCLV1, StCLV2, StBAM1, StBAM2, StCRN, StACR4, StER, or StERL2 gene and the plant is a potato plant.

In certain embodiments, a plant parasitic nematode resistant transgenic plant comprising a transgene wherein a CRN, CLV, or BAM promoter is operably linked to a gene encoding a gene product that is inhibitory to a plant parasitic nematode is provided. In certain embodiments, the gene product is an siRNA directed against a plant parasitic nematode gene. In certain embodiments of any of the aforementioned plants, the CRN, CLV, or BAM promoter is the CRN1, CLV2, or BAM1 promoter sequence provided in Example 3. In certain embodiments of these aforementioned methods, an ACR4, BAM1, BAM2, CLV1, CLV2, CRN, ER, or ERL2 promoter is operably linked to a gene product that is inhibitory to a plant parasitic nematode. In certain embodiments, the gene product is a siRNA or an amiRNA directed against a plant parasitic nematode gene.

In certain embodiments, a recombinant DNA construct comprising a BAM1 promoter that is operably linked to a heterologous gene, wherein said BAM1 promoter comprises any one of: i) the BAM1 promoter sequence provided in Example 3; ii) a promoter that has at least 70%, 85%, 90%, 95%, or 99% sequence identity to the BAM1 promoter sequence provided in Example 3; or ii) a promoter comprising a deletion of about up to about 10, 50, 100, 200, 500, 700, 1000, or 1500 nucleotides of the 5′ nucleotides of the BAM1 promoter sequence provided in Example 3 is provided. In certain embodiments, the BAM promoter is operably linked to a gene encoding a gene product that is inhibitory to a plant parasitic nematode.

DESCRIPTIONS OF THE FIGURES Figure Legends

FIG. 1. Effect of cyst nematode CLE peptides on receptor mutants.

(a) Average root length wild-type (Ler), clv2-1, and crn-1 seedlings grown for 9 days on media with or without the synthetic nematode dodecapeptide CLE motif Data represent the mean±SE, n=10. (b)-(d) Representative roots tips of seedlings grown on media with or without synthetic CLE peptides for 10 days and visualized with differential interference microscopy. (b) No peptide, (c) Sensitive to peptide, and (d) Resistant to peptide. (Scale bar, 50 μm).

FIG. 2. CRN:GUS expression during nematode infection. (a)-(c) GUS expression in uninfected Arabidopsis root tips (a), middle of the root (b), and older part of the root towards the hypocotyl (c). (d)-(g) CRN:GUS expression in response to H. schachtii; early parasitic J2 (d), late parasitic J2 (e), J3 parasitic (f), J4 parasitic (g). Abbreviations: nematode, N; Syn, Syncytium. (Scale bar, 50 μm).

FIG. 3. Confocal images of CLV2:H2B-mCherry expression during nematode infection. (a) J2 parasitic with DIC. (b) J2 parasitic with mCherry fluorescence. (c) J3 parasitic with DIC. (d) J3 parasitic with mCherry fluorescence. Abbreviations: nematode, N; Syn, Syncytium. (Scale bars, 50 μm).

FIG. 4. Effect of clv2-1 and crn-1 mutant alleles on H. schachtii infection.

(a) J4 females were counted at 14 dpi and adult females were counted at 30 dpi. Data represent mean±SE, n=35 for Ler, 32 for crn-1, 34 for clv2-1, and 29 for crn-1 clv2-1. Data are representative of three independent experiments.

(b) Seedlings were grown on vertical square plates for 10 days and inoculated with 10 J2s/root. At 14 dpi, syncytia that fed only one nematode and appeared translucent were microscopically examined and their area was determined. Data represent mean±SE, n=11 for Ler and crn-1, 14 for clv2-1, and 12 for crn-1 clv2-1.

Asterisks indicate statistically significant differences compared to Ler by Student's t test (P<0.05)

FIG. 5. Response of wild-type (Utr) and sol2-1 seedlings to the synthetic 12-aa nematode CLE peptide. Average root length wild-type (Utr) and sol2-1 seedlings grown for 9 days on media with or without the synthetic nematode dodecapeptide CLE motif. Data represent the mean ±SE, n =10.

FIG. 6. Confocal images of nematode autofluorescence in wild-type roots (a and b). Feeding site induced by a parasitic J2. (a) DIC image. (b) mCherry fluorescence. Abbreviations: nematode, N; Syn, Syncytium. (Scale bar 50 μm).

FIG. 7. Effect of sol2-1 mutant allele on Heterodera schachtii infection. (a). J4 females were counted at 14dpi and adult females were counted at 30 dpi. Data represent mean ±SE, n =36. Asterisks indicate statistically significant differences compared to Ler by Student's t test (P <0.0001). Effect of sol2-1 mutant allele on size of syncytia (b). Seedlings were grown on vertical square plates for 10 days and inoculated with 10 ppJ2s/root. At 14 dpi, syncytia that fed only one nematode and appeared translucent were microscopically examined and their area was determined. Data represent mean ±SE, n =11 for Utr and n =9 for sol2-1. Asterisks indicate statistically significant differences compared to Ler by Student's t test (P <0.05).

FIG. 8. Effect of Heterodera glycines (HgCle) and Heterodera schachtii (HsCLE) nematode CLE peptides on receptor mutants. Seedlings were grown for 9 days on media with or without the synthetic nematode dodecapeptide CLE motif. Data represent the mean ±SE, n =10.(A) Average root length of wild-type (Ler), clv2-1, and crn-1. (B) Average root length of wild-type (Col-0,) bam3-2, bam2, and bam1-3. (C) Average root length of wild-type (Utr) and sol2-1.

FIG. 9. Effect of Globodera rostochiensis (GrCLE) nematode CLE peptides on receptor mutants. Seedlings were grown for 9 days on media with or without the synthetic nematode dodecapeptide CLE motif. Data represent the mean ±SE, n =10. Average root length of wild-type (Col-0) bam1-2, bam1-4, erl2-1, and clv2-1.

FIG. 10. Effect of receptor mutant alleles on H. schachtii infection. J4 females were counted at 14dpi and adult females were counted at 30 dpi. Asterisks indicate statistically significant differences compared to wild-type by Student's t test (P <0.05). Data represent mean ±SE, Similar results were obtained from two additional biological replicates.

FIG. 11. CRN:GUS expression during nematode infection. (A-C) CRN: :GUS expression in uninfected Arabidopsis root tips (A), middle of the root (B), and older part of the root towards the hypocotyl (C). (D-G) CRN: :GUS expression in nematode-infected roots; (D) early J2p, (E) late J2p, (F) J3 and (G) J4 females. Abbreviations: nematode, N; Syn, Syncytium.

FIG. 12. CLV2:GUS expression during nematode infection. (A-D) Confocal section of a nematode induced feeding site 4 dpi (A-B) and 8 dpi (A-B) expressing CLV2:H2B-mCherry. Abbreviations: nematode, N; Syn, Syncytium.

FIG. 13. BAM1:GUS expression in Arabidopsis in response to nematode infection. (A) Uninfected root tip. (B-D) GUS expression in nematode infected roots; (B) Early parasitic J2, (C) Parasitic J3, and (D) Parasitic J4.

FIG. 14 Differential expression of candidate potato CLE receptor genes in G. rostochiensis-infected potato roots.

FIG. 15. Effect of crn-1, clv2-6, bam1-3 mutant alleles and combinations thereof on H. schachtii infection in Arabidopsis.

FIG. 16 shows the expression of a pCLV1 promoter fusion to a GUS gene in the vasculature of plants and upregulation at sites of H. schachtii in transgenic Arabidopsis.Abbreviations: nematode, N.

FIG. 17 shows a StCLV2 Potato Promoter:GUS transgenic plant line and activity of this promoter in G. rostochiensis-induced feeding sites.

FIG. 18 shows a StCRN Potato Promoter:GUS transgenic plant line and activity of this promoter in G. rostochiensis-induced feeding sites.

FIG. 19 A shows expression levels of the endogenous StCLV2 gene in transgenic potato plants expressing an artificial miRNA (amiRNA) directed against the StCLV2 gene (3d#29 and 4d#9) and wild type (Wt) control plants that lack the amiRNA.

FIG. 19 B shows the number of G. rostochiensis cysts in transgenic potato plants expressing an artificial miRNA (amiRNA) directed against the StCLV2 gene (3d#29 and 4d#9) and wild type (Wt) control plants that lack the amiRNA.

DESCRIPTION OF THE INVENTION

We describe the use of synthetic CLE peptides, nematode CLE overexpression lines, promoter-reporter lines, and nematode infection assays of receptor mutants to investigate a role for CLV2 and CRN in nematode CLE signaling. Our results indicate that the CLV2/CRN signaling pathway is required for successful nematode infection and syncytium development.

Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ESR(CLE)-like effector proteins. These proteins have been shown to act as ligand mimics of plant CLE peptides and are required for successful nematode infection; however, the receptors for nematode CLE-like peptides have not been identified. Here we demonstrate that CLV2 and CORYNE (CRN), members of the receptor kinase family, are required for nematode CLE signaling. Exogenous peptide assays and overexpression of nematode CLEs in Arabidopsis showed that CLV2 and CRN are required for nematode CLE perception. In addition, promoter-reporter assays showed that both receptors are expressed in nematode-induced syncytia. Lastly, infection assays with receptor mutants revealed a decrease in both nematode infection and syncytia size. Taken together, our results indicate that nematode CLE perception by CLV2 and CRN is not only required for successful nematode infection, but is also involved in the formation or maintenance of nematode-induced syncytia.

Plant Nematode CLE Receptor Genes that can be Used to Obtain Nematode Resistant Plants and Methods of Use

A variety of plant nematode CLE peptide receptor genes (hereinafter referred to as “PNCLEPRG”) that provide for inhibition of plant parasitic nematode infections are provided herewith, along with associated methods of use, and plants comprising transgenes or mutations wherein expression of the PNCLEPRG are inhibited. Reduced expression of the PNCLEPRG in plants inhibits infection of the plants by nematodes. Such reductions in nematode infection result in improved plant yield and plant product quality.

Reductions in expression of the endogenous PNCLEPRG can be effected by any method that at least provides for reductions in the amount or activity of the PNCLEPRG at the site of nematode infection in the plant. Such sites of infection are commonly the plant roots, but can also comprise other plant parts such as tubers.

In certain embodiments, inhibition of PNCLEPRG expression in a plant can be effected by transgenes. Such transgenes include, but are not limited to, transgenes that: i) produce an siRNA directed against the PNCLEPRG; ii) produce an artificial microRNA targeting the PNCLEPRG; iii) produce a dominant negative form of the protein product of the PNCLEPRG; iv) produce an antisense or sense form of the PNCLEPRG; or v) comprise a genomic insertion that disrupts the endogenous PNCLEPRG.

Exemplary vector systems that can provide for production of siRNA in plants include, but are not limited to, vectors disclosed by Dafny-Yelin, et al. (Plant Physiology, 2007, Vol. 145, pp. 1272-1281), Wesley et al. 2001, Plant J. 27: 581-590, and Miki and Shimamoto, (2004) Plant Physiol 138: 1903-1913. Vectors for producing an siRNA are also described in U.S. Pat. No. 6,635,805, incorporated herein by reference in its entirety.

Exemplary vector systems that can provide for production of artificial miRNA in plants include, but are not limited to, vectors disclosed by Warthmann et al. (2008) PLoS ONE 3(3): e1829. doi:10.1371/journal.pone.0001829; and Alvarez et al. (2006) Plant Cell 18: 1134-1151. Vectors for effecting efficient inhibition of endogenous plant genes by expression of hairpin RNAs are also disclosed in U.S. Patent Application Nos. 20050164394, 20050160490, and 20040231016, each of which is incorporated herein by reference in their entirety. Exemplary dominant negative mutations that can provide for inhibition endogenous PNCLEPRG include, but are not limited, mutations modeled after dominant negative mutations in other Leucine Rich Repeat-Receptor Like Kinase (LRR-RLK) proteins.

In one embodiment, the dominant negative mutation can comprise a deletion or other loss-of-function mutation in the kinase domain. Such mutations have been disclosed for plant LRR-RLK proteins (Shpak et al., Plant Cell, Vol. 15, 1095-1110, 2003). Methods of identifying transgene insertions into specific genomic loci have also been disclosed. T-DNA of Agrobacterium is also an insertional mutagen that can be used as an agent to reduce expression of an endogenous PNCLEPRG. T-DNA mutagenesis has been described in Arabidopsis (Krysan et al., Plant Cell, 1999, 1: 2283-2290) and rice (Jeon et al., Plant J. June 2000; 22(6):561-70). Transposons such as those in the Ac/Ds (Activator-Disassociation) family and the Enhancer-inhibitor system can also be used to effect mutagenesis of an endogenous PNCLEPRG. Transposon mutagenesis schemes have been described (Speulman et al. Plant Cell, Vol. 11, 1853-1866, October 1999; Das, L., and Martienssen, R, 1995, Plant Cell 7:287-294).

Plants wherein expression of the endogenous PNCLEPRG is inhibited by a mutation and the use of such plants is also provided. Methods of identifying plants comprising mutations in PNCLEPRG include, but are not limited to, “TILLING” (Targeting Induced Local Lesions in Genomes). The TILLING technique comprises the induction of mutations across the genome followed by the identification and isolation of plants with mutations in desired genes (McCallum, Plant Physiology, 2000, Vol. 123, pp. 439-44).

PNCLEPRG target genes useful in the methods and plants of this invention include, but are not limited to, the ACR4, CLV1, CLV2, CRN, BAM1, BAM2, ER, and ERL2 genes of Arabidopsis and the orthologous ACR4, CLV1, CLV2, CRN, BAM1, BAM2, ER, and ERL2 genes of crop and ornamental plants subject to nematode infestation. Such orthologous genes are referred to herein as “ACR4-like, CLV1-like, CLV2-like, CRN-like, BAM1-like, BAM2-like, ER-like, and ERL2-like” genes. As used herein, the terms “orthologous” and “-like” (when appended to a gene) thus refer to genes that at least have a similar role in plant nematode CLE peptide signal transduction in their respective plant species of origin. In certain embodiments, the PNCLEPRG target genes are obtained from a plant that is a monocot or dicot plant, or that is a crop plant such as a tobacco, cereal, sugar beet, cotton, fruit, fiber, oilseed, potato, rice, corn, soybean, vegetable, and wheat plant. Exemplary vegetable plants include, but are not limited to, carrot, pepper, cucurbit, and tomato plants.

In certain embodiments, the PNCLEPRG target genes are derived from the plant that will be used (i.e. protected from nematode infection). However, a PNCLEPRG of a given plant specie can be used in a distinct plant species when it has sufficient homology to the orthologous PNCLEPRG of a distinct plant species. In this context, “sufficient homology” is that amount of homology necessary to provide for transgene-mediated inhibition of the orthologous gene. For certain transgene-mediated gene inhibition methods, a PNCLEPRG sequence of about is 23 nucleotides or longer with least 80%, 85%, 90%, 95%, 98%, 99% or 100% identity to the target orthologous sequence can be used. In certain embodiments, a hairpin RNA may comprise a 5′ sequence of roughly 19-24 nucleotides of sense strand target gene sequence with 100% identity followed by a spacer nucleotide of about 8-10 nucleotides followed by a sequence of roughly 19-24 nucleotides of antisense sequence that is capable of base pairing with the preceding sense strand sequence. In certain embodiments, a 19-24 base region of a PNCLEPRG that exhibits 100% identity over 19-24 nucleotides to an orthologous PNCLEPRG can also be used to inhibit that orthologous gene.

In certain embodiments, an Arabidopsis PNCLEPRG can be used to obtain nematode resistant plants, where the plants are Arabidopsis or other plants that comprise orthologous PNCLEPRGs that can be inhibited by the Arabidopsis PNCLEPRG. Arabidopsis PNCLEPRG include, but are not limited to, the ACR4, CLV1, CLV2, CRN, BAM1, BAM2, ER, and ERL2 can in certain embodiments be used to control plant parasitic nematode infections of cruciferous plants that include, but are not limited to, arugula, cauliflower, cabbage, cress, bok choy, broccoli, radish, canola, turnip, watercress, and the like.

In certain embodiments, a potato PNCLEPRG can be used to obtain nematode resistant plants, where the plants are potato plants or other plants that comprise orthologous PNCLEPRGs that can be inhibited by the potato PNCLEPRG. Potato PNCLEPRG provided herein include, but are not limited to, stCRN (SEQ ID NO:6), stBAM1 (SEQ ID NO:7), stBAM2 (SEQ ID NO:8), stER (SEQ ID NO:9), stCLV1 (SEQ ID NO:10), stCLV2 (SEQ ID NO:11), stACR4 (SEQ ID NO:12), and stERL2 (SEQ ID NO:13). Also provided herewith are related sequences with at least 70%, 80%, 85%, 90%, 95%, 97%, 98%, or 99% identity to stCRN (SEQ ID NO:6), stBAM1 (SEQ ID NO:7), stBAM2 (SEQ ID NO:8), stER (SEQ ID NO:9), stCLV1 (SEQ ID NO:10), stCLV2 (SEQ ID NO:11), stACR4 (SEQ ID NO:12), and stERL2 (SEQ ID NO:13) as well as methods of using such sequences to control plant nematodes.

In certain embodiments, the use of such potato PNCLEPRGs and related sequences to control plant nematode, and particularly, plant cyst nematode infections, in solanaceous plants including, but not limited to, eggplant, tobacco, potato, and tomato is provided. In certain embodiments, the use of such potato PNCLEPRGs and related sequences to control Globedera sp. infections of potato plants is provided. In any of the aforementioned embodiments, inhibition of the plant PNCLEPRG can be limited to inhibition in roots or limited to inhibition at the site of nematode infection by use of root-specific and/or nematode inducible promoters, respectively.

In certain embodiments, a soybean PNCLEPRG can be used to obtain nematode resistant plants, where the plants are soybean plants or other plants that comprise orthologous PNCLEPRGs that can be inhibited by the soybean PNCLEPRG. Soybean PNCLEPRG provided herein include, but are not limited to, soybean CRN (SEQ ID NO:44, 45, 47, and 48), BAM1 (SEQ ID NO:23, 24, 26, 27), BAM2 (SEQ ID NO:29, 30, 32, 33), CLV1 (SEQ ID NO:38, 39, 41, 42), and CLV2 (SEQ ID NO:35, 36, 50, 51) orthologs. Also provided herewith are related sequences with at least 70%, 80%, 85%, 90%, 95%, 97%, 98%, or 99% identity to soybean CRN (SEQ ID NO:44, 45, 47, 48), BAM1 (SEQ ID NO:23, 24, 26, 27), BAM2 (SEQ ID NO:29, 30, 32, 33), CLV1 (SEQ ID NO:38, 39, 41, 42), and CLV2 (SEQ ID NO: 35, 36, 50, 51) orthologs as well as methods of using such sequences to control plant nematodes. In certain embodiments, the use of such soybean PNCLEPRGs and related sequences to control plant nematodes, and particularly, plant cyst nematode infections, in leguminous plants including, but not limited to, alfalfa, clover, peas, beans, lentils, lupins, mesquite, carob, soybean, and peanuts, is provided. In certain embodiments, the use of such soybean PNCLEPRGs and related sequences to control Heterodera glycine infections of soybean plants is provided. In any of the aforementioned embodiments, inhibition of the plant PNCLEPRG can be limited to inhibition in roots or limited to inhibition at the site of nematode infection by use of root-specific and/or nematode inducible promoters, respectively.

In certain embodiments of the invention, combinations of two or more a plant PNCLEPRG are inhibited in a plant to provide resistance to plant parasitic nematode infections. Plants wherein combinations of two or more PNCLEPRG selected from the group of CRN, BAM1, BAM2, ER, CLV1, CLV2, ACR4, and ERL2 genes or orthologs thereof are inhibited can be used to provide resistance to plant parasitic nematode infections. In certain embodiments, a plant CLV2-like and a plant BAM1-like gene are both inhibited in parallel to reduce nematode infections in the plant. In certain embodiments of the invention, a plant CRN-1-like and a plant BAM1-like gene are both inhibited in parallel to reduce nematode infections in the plant. In certain embodiments, combinations of two or more of a soybean CRN (SEQ ID NO:44, 45, 47, and 48), BAM1 (SEQ ID NO:23, 24, 26, 27), BAM2 (SEQ ID NO:29, 30, 32), CLV1 (SEQ ID NO:38, 39, 41, 42), and CLV2 (SEQ ID NO:35, 36, 50, 51) orthologs or a related sequence are inhibited in a soybean or other plant to provide resistance to plant parasitic nematode infections. In certain embodiments, combinations of two or more of a potato stCRN (SEQ ID NO:6), stBAM1 (SEQ ID NO:7), stBAM2 (SEQ ID NO:8), stER (SEQ ID NO:9), stCLV1 (SEQ ID NO:10), stCLV2 (SEQ ID NO:11), stACR4 (SEQ ID NO:12), and stERL2 (SEQ ID NO:13) are inhibited in a potato or other plant to provide resistance to plant parasitic nematode infections. In any of the aforementioned embodiments, inhibition of the plant PNCLEPRG can be limited to inhibition in roots or limited to inhibition at the site of nematode infection by use of root-specific and/or nematode inducible promoters, respectively.

In addition to nematode resistant plants, the instant invention also provides for parts of those plants and plant cells. Plant parts provided herein include, but are not limited to, seeds, tubers, roots, leaves, stalks, lint, and the like. Also provided herein are processed products of the nematode resistant plants. Such processed plant products include, but are not limited to, a ground meal, a feed, a cake, and the like. In certain embodiments, such processed product would comprise a detectable amount of a transgene used to inhibit the PNCLEPRG.

Promoters from Plant Nematode CLE Receptor Genes and Methods of Use

Promoters from PNCLEPRG and recombinant DNA constructs providing such promoters that are useful for expressing genes of interest in plant cells where the nematodes feed are provided. Such promoters are particularly useful for expressing nucleic acid and/or protein sequences that are inhibitory to plant parasitic nematodes. Particular advantages of the promoters include, but are not limited to, providing for expression of the operably linked nucleic acid sequences at nematode feeding sites within the plant while limiting expression of the gene in other parts of the plant where such expression is not required or desired. As used herein in the context of a promoter, the term “operably linked” means that a promoter is connected to a sequence of interest such that the transcription of that sequence of interest is controlled and regulated by that promoter. When the sequence of interest encodes a protein and when expression of that protein is desired, “operably linked” means that the promoter is linked to the sequence in such a way that the resulting transcript will be efficiently translated. If the linkage of the promoter to the coding sequence is a transcriptional fusion and expression of the encoded protein is desired, the linkage is made so that the first translational initiation codon in the resulting transcript is the initiation codon of the coding sequence. Alternatively, if the linkage of the promoter to the coding sequence is a translational fusion and expression of the encoded protein is desired, the linkage is made so that the first translational initiation codon contained in the 5′ untranslated sequence associated with the promoter and is linked such that the resulting translation product is in frame with the translational open reading frame that encodes the protein desired.

A variety of recombinant DNA molecules comprising promoters of the invention that are operably linked to heterologous genes or nucleic acids of interest are provided. Heterologous genes or nucleic acids that provide for inhibition of plant parasitic nematodes can be operably linked to the PNCLEPRG promoters. In certain embodiments, the heterologous genes or nucleic acids of interest provide for inhibition of a plant parasitic nematode gene or function. Such plant parasitic nematode genes or functions include, but are not limited to, nematode genes that are essential or required for nematode viability or nematode genes involved in any aspect of plant host parasitism. In certain embodiments, the promoters are used to drive expression of heterologous genes or nucleic acids that are inhibitory to nematode genes disclosed in US Patent Application publication US20090012029, which discloses inhibitory nucleic acid specific for one or more cyst nematode esophageal gland cell proteins and which is incorporated herein by reference in its entirety.

In certain embodiments, the promoters are used to drive expression of genes or nucleic acids that inhibit formation and/or maintenance of the plant cells of the nematode feeding site. In certain embodiments, the promoters are thus used to: i) drive expression of heterologous genes or nucleic acids that are inhibitory to endogenous plant genes involved in formation and/or maintenance of the plant cells of the nematode feeding site; and/or, ii) drive expression of heterologous genes that comprise endogenous plant genes that are downregulated during the formation and/or maintenance of the plant cells of the nematode feeding site. Endogenous plant genes involved in formation and/or maintenance of the plant cells of the nematode feeding site that include, but are not limited to, genes involved in the cell wall architectural modifications during feeding site formation/maintenance, genes involved in sugar or carbohydrate, metal ion, and amino acid transport, and genes involved in plant phytohormone signaling and biosynthesis. A variety of soybean plant genes suitable for use with the promoters of the invention are disclosed in Ithal et al., Molec. Plant. Microb. Interact. Vol. 20, No. 5, 2007, pp. 510-525, incorporated herein by reference in its entirety. PNCLEPRG promoters useful in the methods and plants of this invention include, but are not limited to, the ACR4, CLV1, CLV2, CRN, BAM1, BAM2, ER, and ERL2 promoters of Arabidopsis and the orthologous ACR4, CLV1, CLV2, CRN, BAM1, BAM2, ER, and ERL2 promoters of crop and ornamental plants subject to nematode infestation. Such orthologous promoters are referred to herein as “ACR4-like, CLV1-like, CLV2-like, CRN-like, BAM1-like, BAM2-like, ER-like, and ERL2-like” promoters. As used herein, the terms “orthologous” and “-like” (when appended to a promoter) thus refer to promoters that at least have a similar role or expression pattern in plant nematode CLE peptide signal transduction in their respective plant species of origin. In certain embodiments, the PNCLEPRG promoters are obtained from a plant that is a monocot or dicot plant, or that is a crop plant such as a tobacco, cereal, sugar beet, cotton, fruit, fiber, oilseed, potato, rice, corn, soybean, vegetable, and wheat plant. Exemplary vegetable plants include, but are not limited to, carrot, pepper, cucurbit, and tomato plants.

In certain embodiments, a recombinant DNA construct comprising a PNCLEPRG promoter that is operably linked to a heterologous gene, or a plant, plant cell, plant part, or processed plant product comprising the same, is provided. In certain embodiments, the PNCLEPRG promoter comprises any one of: i) a potato ACR4 promoter (SEQ ID NO:15), an Arabidopsis (SEQ ID NO:14), potato (SEQ ID NO:16), or soybean (SEQ ID NO: 38 or 41) CLV1 promoter; ii) an Arabidopsis (SEQ ID NO:4), potato (SEQ ID NO:17), or soybean (SEQ ID NO:35 or 50) CLV2 promoter; iii) an Arabidopsis (SEQ ID NO:5), potato (SEQ ID NO:18), or soybean (SEQ ID NO: 44 or 47) CRN promoter; iv) an Arabidopsis (SEQ ID NO:3), potato (SEQ ID NO:19), or soybean (SEQ ID NO: 23 or 26) BAM1 promoter; v) a potato (SEQ ID 20), or soybean (SEQ ID NO: 29 or 32) BAM2 promoter; vi) a potato ER promoter (SEQ ID NO:21); vii) or a potato ERL2 promoter (SEQ ID NO:22). Also provided are recombinant DNA constructs comprising a variant PNCLEPRG promoter that has at least 70%, 85%, 90%, 95%, or 99% sequence identity to any one of: i) a potato ACR4 promoter (SEQ ID NO:15), an Arabidopsis (SEQ ID NO:14), potato (SEQ ID NO:16), or soybean (SEQ ID NO: 38 or 41) CLV1 promoter; ii) an Arabidopsis (SEQ ID NO:4), potato (SEQ ID NO:17), or soybean (SEQ ID NO:35 or 50) CLV2 promoter; iii) an Arabidopsis (SEQ ID NO:5), potato (SEQ ID NO:18), or soybean (SEQ ID NO: 44 or 47) CRN promoter; iv) an Arabidopsis (SEQ ID NO:3), potato (SEQ ID NO:19), or soybean (SEQ ID NO: 23 or 26) BAM1 promoter; v) a potato (SEQ ID 20), or soybean (SEQ ID NO: 29 or 32) BAM2 promoter; vi) a potato ER promoter (SEQ ID NO:21); vii) or a potato ERL2 promoter (SEQ ID NO:22).

In certain embodiments, recombinant DNA constructs comprising a PNCLEPRG promoter comprising a deletion of about up to about 10, 50, 100, 200, 500, 700, 1000, or 1500 nucleotides of the 5′ nucleotides of any one of: i) a potato ACR4 promoter (SEQ ID NO:15), an Arabidopsis (SEQ ID NO:14), potato (SEQ ID NO:16), or soybean (SEQ ID NO: 38 or 41) CLV1 promoter; ii) an Arabidopsis (SEQ ID NO:4), potato (SEQ ID NO:17), or soybean (SEQ ID NO:35 or 50) CLV2 promoter; iii) an Arabidopsis (SEQ ID NO:5), potato (SEQ ID NO:18), or soybean (SEQ ID NO: 44 or 47) CRN promoter; iv) an Arabidopsis (SEQ ID NO:3), potato (SEQ ID NO:19), or soybean (SEQ ID NO: 23 or 26) BAM1 promoter; v) a potato (SEQ ID 20), or soybean (SEQ ID NO: 29 or 32) BAM2 promoter; vi) a potato ER promoter (SEQ ID NO:21); vii) or a potato ERL2 promoter (SEQ ID NO:22) is provided. Those skilled in the art will appreciate that promoter and 5′UT regions of PNCLEPRG provided herewith as genomic sequences in association with the coding regions can be dissociated from those coding regions and operably linked to heterologous nucleic acids or genes by transcriptional or translational fusions. In certain embodiments, the soybean PNCLEPRG promoters and 5′UT of Table 5 (SEQ ID NO: 23, 26, 29, 32, 35, 38, 41, 44, 47, and 50) thus comprise the nucleic acid sequences located 5′ to the start codon of those genomic sequences.

In certain embodiments, variants of any of the aforementioned PNCLEPRG promoters comprising at least about 300, 500, 800, 900, 1,000, 1,500, 2,500, or 3,000 nucleotides of the nucleic acid sequence located 5′ to the start codon or located 5′ to mRNA 5′ cap site of the endogenous gene associated with said promoter are provided. Also provided are recombinant DNA constructs wherein any of the aforementioned promoters is operably linked to a gene encoding a gene product that is inhibitory to a plant parasitic nematode.

In addition to nematode resistant plants comprising the recombinant DNA constructs of the aforementioned PNCLEPRG promoters, the instant invention also provides for parts of those plants and plant cells. Plant parts provided herein include, but are not limited to, seeds, tubers, roots, leaves, stalks, lint, and the like. Also provided herein are processed products of the nematode resistant plants. Such processed products include, but are not limited to, a ground meal, a feed, a cake, and the like.

In certain embodiments, such processed product would comprise a detectable amount of a recombinant DNA comprising a PNCLEPRG promoter that is operably linked to a heterologous gene.

EXAMPLES

The disclosed embodiments are merely representative of the invention, which may be embodied in various forms. Thus, specific structural and functional details disclosed herein are not to be interpreted as limiting.

Example 1 Experimental Procedures

Peptide Assays

Arabidopsis seeds were sterilized using the chlorine gas method (Wang et al., 2010b). Sterilized seeds were germinated on vertical plates in a growth chamber at 22° C. under long-day conditions (16 h light/8 h dark) containing synthetic peptides (Sigma-Genosys) as previously described (Wang et al., 2010b). The clv2-1 mutant in the Ler background (Koornneef et al., 1983) was obtained from the Arabidopsis Biological Resource Center. The crn-1 mutant in the Ler background (Muller, 2008) and the sol2-1 mutant in the Utr background (Miwa et al., 2008) have been described previously. The HgCLEp, HsCLE1p, and HsCLE2p peptides used in this study were as described (Wang et al., 2010b). Two days after germination, root length was marked each day for nine days. Plates were scanned using an Epson Perfection V200 PHOTO scanner and total root length was determined using Scion Image. Primary root tips of Arabidopsis were mounted on glass slides and visualized with an Olympus Vanox AHBT3 microscope equipped with Nomarski optics.

Overexpression in Mutant Backgrounds

The CLE gene sequences from the soybean cyst nematode (HgCLE2^(ΔSP)) and the beet cyst nematode (HsCLE1 and HsCLE2) used to generate the overexpression constructs were previously described (Wang et al., 2010a; Wang et al., 2010b). Constructs were transformed into the mutant backgrounds using the Arabidopsis floral dip method (Clough and Bent, 1998). Seeds from primary Arabidopsis transformants (T1) were selected on 0.5×MS media [MS basal nutrients salts (Caisson Laboratories), 2% sucrose, 0.8% Type A agar (Sigma), pH 5.7] containing 50 μg/mL timentin (GlaxoSmithKline) to control Agrobacterium contamination, and 50 μg/mL kanamycin and grown under the same conditions as above. Seedlings resistant to kanamycin were transplanted to soil seven days after germination. Two weeks after transplanting to soil the shoot phenotypes were observed.

Promoter-Reporter Lines

CRN:GUS has been previously described and characterized (Muller et al., 2008). To generate CLV2:H2B-mCherry, vector pMDC99 (Curtis and Grossniklaus, 2003) was modified by introducing the CDS of chimeric construct mCherry-H2B at the 3′ site of the gateway cassette using the unique Pad restriction site to give pAB149. To analyze the expression of CLV2 1252 bp of the 5′ region and 9 bp of the CDS was amplified using the primers AB_CLV2_Pro_F (5′ CACCAGACACAAAGCCCTTTCCATTGTC 3′; SEQ ID NO:1) and AB_CLV2_Pro_R (5′ CTTTATCATAGCTCAGAGGA 3′; SEQ ID NO:2) to give a CACC-TOPO containing amplicon, which was cloned into pENTR/D-TOPO (Invitrogen™). This entry clone was used in a LR reaction with pAB149 to give pAB183 (CLV2:H2B-mCherry). Expression of CLV2 under the control of the endogenous promoter, using 1252 bp of the CLV2 5′ region was sufficient to rescue the clv2-1 mutant in all isolated lines (N=20).

Nematode Infection of Promoter-Reporter Lines

The beet cyst nematode (BCN) Heterodera schachtii was propagated on greenhouse-grown sugar beets (Beta vulgaris cv Monohi). BCN eggs were isolated and hatched as previously described (Mitchum et al., 2004). After 2 days, second stage juveniles (J2) were collected and surfaced sterilized according to Wang et al. (2007) except 0.004% mercuric chloride, 0.004% sodium azide, and 0.002% Triton X-100 were used. Sterilized seeds were grown on modified Knop's medium (Brunschwig Chemie) (Sijmons et al., 1991). Ten days after germination seedlings were inoculated with 20 sterilized J2/root.

Histochemical β-Glucuronidase (GUS) Assays

At the indicated timepoints, freshly excised CRN:GUS tissues were infiltrated with GUS substrate buffer (0.5 mM 5-bromo-4chloro-3-indolyl glucuronide, 100 mM Tris, pH 7.0, 50 mM NaCl, 0.06% Triton X-100, 3 mM potassium ferricyanide) and incubated overnight at 37° C. (Jefferson et al., 1987). Stained roots were placed in glass Petri dishes and visualized with a Nikon Eclipse TS 100 inverted microscope.

Confocal Microscopy

CLV2:H2B-mCherry seed was sterilized, grown, and inoculated with nematodes as described above. At the indicated timepoints, infected roots were mounted on glass slides and visualized with a 510 META confocal scanning microscope (Carl Zeiss, Thornwood, N.Y., USA) excited at 543 nm.

Infection Assay with Receptor Mutants

Sterilized receptor mutants were plated in 12-well Falcon tissue culture plates (BD Biosciences) containing modified Knop's medium with 0.8% Daishin agar in a randomized block design. Plants were grown at 24° C. with a 12 hour photoperiod. Fourteen days after germination, seedlings were inoculated with 200 surface-sterilized BCN J2. J4 females were counted at 14 days post-inoculation (dpi) and adult females were counted at 30 dpi. The average values were calculated and significant differences were determined by using Student's t test (P<0.05). To measure syncytia size, receptor mutants were germinated on modified Knop's medium in vertical square plates and inoculated at 10 days after germination with 10 surface-sterilized BCN J2. At 14 dpi, syncytia that were transparent and only fed upon by only one nematode were visualized with a Nikon Eclipse TS 100 inverted microscope. Area of syncytia was measured using Adobe Photoshop CS5 and significant differences were determined by using Student's t test (P<0.05).

Results

CLV2 and CRN are Required for Nematode CLE Perception

We have previously shown that exogenously applied 12-aa peptides corresponding to the CLE motifs of the SCN (HgCLEs) and the BCN (HsCLEs) CLEs can function as plant CLE peptide mimics causing termination of the primary root meristem in a concentration dependent manner (Wang et al., 2010b). In Arabidopsis, it has been shown that the short root phenotype caused by overexpression or exogenous application of some plant CLE peptides is dependent on CLV2 signaling (Fiers et al., 2005; Miwa et al., 2008; Muller, 2008; Meng et al., 2010). More recent evidence indicates that CLV2 forms a complex with CRN and can transmit the signal from CLV3 binding in a CLV1-independent manner (Miwa et al., 2008; Muller, 2008; Bleckmann et al., 2010; Zhu et al., 2010). To determine whether or not CLV2 and CRN might play a role in cyst nematode CLE perception we screened the Arabidopsis clv2-1 null mutant and the crn-1 amorphic allele for resistance to the HgCLE, HsCLE1, and HsCLE2 12-aa peptides. Seeds were grown on vertical plates in the absence of exogenous peptide or in the presence of 1 μM HgCLE or 10 μM of the HsCLEs and roots were measured 9 days after germination. Wild-type seedlings (Landsberg erecta [Ler]) had significantly shorter roots when grown on plates with any of the CLE peptides in comparison to the no peptide control (FIG. 1a ). In contrast, clv2-1 and crn-1 root growth was relatively unimpaired in the presence of the different CLE peptides (FIG. 1a ). The same observation was made with sol2-1, another mutant allele of CRN (Miwa et al., 2008) (FIG. 5). Previous reports have indicated that the short root phenotype can be attributed to a decrease in the number of meristematic cells (Fiers et al., 2005). Using Nomarski optics we confirmed that clv2-1 and crn-1 were insensitive to peptide application resulting in root meristems that were indistinguishable from the no peptide control (FIG. 1b-d ).

Nematode CLEs function in planta through a CLV2- and CRN-dependent pathway Overexpression of HgCLE2, HsCLE1, and HsCLE2 in wild-type Arabidopsis has been shown to cause wus-like phenotypes similar to other plant CLEs (Strabala et al., 2006; Meng et al., 2010; Wang et al., 2005; Wang et al., 2010a; Wang et al., 2010b). If CLV2 and/or CRN are involved in nematode CLE perception then we would expect the phenotypes to be diminished or abolished when overexpressed in clv2-1 and/or crn-1. Each of the nematode CLE genes was cloned into an overexpression vector and transformed into the mutant backgrounds. Transgenic seedlings in the T1 generation were screened and characterized in soil. In contrast to the overexpression phenotypes seen in wild-type Arabidopsis where a high percentage of wus-like phenotypes were observed (Wang et al., 2010a; Wang et al., 2010b), no wus

-like phenotypes were observed when HgCLE2, HsCLE 1, and HsCLE2 were overexpressed in clv2-1 or crn-1 (Table 1). These results demonstrate that mutations in CRN and CLV2 suppress nematode CLE overexpression phenotypes.

TABLE 1 Summary of nematode CLE overexpression phenotypes in clv2-1 and crn-1. T1 Shoot Phenotypes Background Construct wus-like (%) WT (%) Total T1 (#) clv2-1 HgCLE2 0 100 96 HsCLE1 0 100 67 HsCLE2 0 100 28 crn-1 HgCLE2 0 100 85 HsCLE1 0 100 41 HsCLE2 0 100 37

Spatial and temporal relationship between CLV2, CRN, and nematode feeding sites Cyst nematodes enter the root near the zone of elongation, migrate through root cortical cells using their stylet to puncture through cell walls, and begin feeding from a single cell near the vascular cylinder. Once cyst nematodes initiate a feeding site the dorsal esophageal gland cell becomes active and the secreted CLE peptides are delivered to the host root cells (Wang et al., 2010a). In order for CLV2 and CRN to be able to perceive the nematode CLE as a ligand mimic they must be expressed in the correct spatial and temporal context.

Using a CRN:GUS transgene in Arabidopsis, CRN expression was previously shown to be expressed throughout the root including the vasculature where the nematode initiates feeding (FIG. 2a-c ; Muller et al., 2008). To confirm whether CRN is expressed in nematode feeding sites, transgenic Arabidopsis seedlings expressing CRN:GUS were infected with BCN and monitored during nematode development. GUS expression was detected in feeding sites as soon as early second-stage juveniles (J2) began to feed. (FIG. 2d ). GUS expression reached its peak once nematodes reached late J2 parasitic stages, but remained detectable in the feeding sites of third stage juvenile (J3) parasitic nematodes (FIGS. 2e and f ). By the time the nematodes reached the fourth stage juvenile (J4) life stage, GUS expression was either weak or absent in feeding sites (FIG. 2g ).

Similar to CRN, CLV2 is expressed in many different vegetative tissues (Jeong et al., 1999). However little is known about the expression pattern of CLV2 in roots. To visualize CLV2 expression in roots and nematode feeding sites, mCherry was fused to the C-terminus of the Arabidopsis Histone 2B (H2B) gene and placed under the transcriptional control of the CLV2 promoter. The H2B protein has been shown to be a valid marker for chromatin organization in plant nuclei and has been used to describe development of the syncytial endosperm in Arabidopsis (Boisnard-Lorig et al., 2001). In uninfected roots, CLV2:H2B-mCherry fluorescence was detected throughout the root vasculature with the strongest expression detected in lateral root primordia and the zone of elongation extending down to the root apical meristem (A. Bleckmann and R. Simon, unpublished). Upon nematode infection, increased expression of CLV2:H2B-mCherry fluorescence was detected in the nuclei of syncytia fed upon by parasitic J2s (FIG. 3a-b ). At the J3 life stage CLV2:H2B-mCherry continued to be specifically expressed within feeding sites (FIG. 3c-d ). No fluorescence was detected in nuclei of syncytia fed upon by parasitic J2s in wild-type plants (FIG. 6a-b ).

Mutant alleles of CLV2 and CRN cause a reduction in nematode infection and defects in syncytial size.

By using an RNAi approach targeting nematode CLE genes, previous reports have shown that nematode CLE peptides are important for successful infection of host plants roots (Bakhetia et al., 2007; Patel et al., 2008). To determine if nematode CLE perception by CLV2 or CRN is required, root infection assays with nematodes were performed on the clv2-1 and crn-1 single mutants, and the crn-1 clv2-1 double mutant. According to Muller et al. (2008), crn-1 clv2-1 is morphologically indistinguishable from either of the single mutants, indicating that they act in the same pathway. The mutant alleles and the wild-type Ler were randomized in 12-well plates and grown on modified Knop's medium.

Two weeks after germination seedlings were inoculated with infective J2s. J4 females were counted at 14 days post-inoculation (dpi) and adult females were counted at 30 dpi. Both the single and double mutants showed a statistically significant reduction in nematode infection with the exception of crn-1 at 14 dpi (FIG. 4a ). At 30 dpi nematode infection was reduced by approximately 25% in all receptor mutants tested. A similar reduction in nematode infection across all mutant lines supports the hypothesis that CLV2 and CRN are acting in the same signaling pathway. Using sol2-1, we observed a 40% reduction in nematode infection (FIG. 7a ). Since the establishment of a feeding site is required for nematode development and reproduction, the above observations motivated us to determine if there were any defects in syncytial size between the receptor mutants and wild-type.

The mutant alleles and the wild-type Ler were grown on vertical square plates and inoculated with infective J2s. At 14 dpi, syncytia that were transparent and fed upon by only one nematode were measured. The average area of wild-type (Ler) syncytia was 1402±147 μm2 (FIG. 4b ). In contrast, the syncytia of the receptor mutant alleles were reduced by approximately 40%. The average area of crn-1, clv2-1, and crn-1 clv2-1 was 797±89 μm2, 745±61 μm2, and 808±57 μm2, respectively (FIG. 4b ). The same reduction in syncytia size was seen in the sol2-1 mutant allele (FIG. 7b ).

Nematode CLE genes have been found to be upregulated in the dorsal esophageal gland cell at the onset of parasitism and remain on through the adult female life stage. CLE genes are turned off in adult males that are no longer feeding (Wang et al., 2005; Patel et al., 2008; Lu et al., 2009; Wang et al., 2010a). In SCN and BCN, immunolocalization studies have localized nematode CLEs along the dorsal gland extension and in the ampulla at the base of the nematode stylet indicating they are secreted into host plant roots via the stylet (Wang et al., 2005; Patel et al., 2008; Wang et al., 2010a). Consistent with these results an immunofluorescence study found that SCN CLEs are secreted directly into host plant root cytoplasm (Wang et al., 2010a). The variable domain of SCN CLEs is then able to redirect the proteins into the apoplast where they can act as plant CLE ligand mimics by interacting with extracellular membrane bound plant CLE receptors. However, thus far, host plant receptors that perceive nematode CLE signals have not been identified.

Many studies have used synthetic CLE peptides to help determine the roles that plant CLE peptides play in plant growth and development. Previous studies have shown that nematode CLE peptides cause root growth phenotypes similar to other plant CLEs (Lu et al., 2009; Wang et al., 2010a; Wang et al., 2010b). Other studies have also shown that these peptide screens can identify receptors that may be involved in certain CLE signaling pathways by utilizing receptor mutants (Fiers et al., 2005; Stahl et al., 2009; Meng et al., 2010).

To identify potential nematode CLE receptors we tested plant CLE receptors implicated in CLE signaling in the RAM for a role in nematode CLE perception. In the root, exogenous peptide assays and overexpression studies have shown that CLV2 is required for proper proximal meristem function (Stahl et al., 2009; Meng et al., 2010).

It has also been shown that a new member of the receptor kinase family, CRN, forms a heterodimer with CLV2 and is required for proper localization of the CLV2/CRN complex to the plasma membrane (Bleckmann et al., 2010; Zhu et al., 2010). In Arabidopsis, CRN has been found to be widely expressed in both shoot and root tissues suggesting dual roles in shoot and root development (Muller et al., 2008). CLV2 has been found to be expressed in shoot tissues (Jeong et al., 1999), but less is known about its expression in the root. In this paper we screened a null mutant allele of CLV2 and an amorphic mutant allele of CRN for resistance to the nematode CLE peptides. Both clv2-1 and crn-1 were resistant to HgCLEp, HsCLE1p, and HsCLE2p (FIGS. 1 and 5). Similar to synthetic peptide assays, overexpression of HgCLE, HsCLE1, and HsCLE2 in the clv2-1 and crn-1 mutant backgrounds abolished the wus-like phenotypes seen when the nematode CLEs are overexpressed in wild-type backgrounds (Wang et al., 2005; Wang et al., 2010a; Wang et al., 2010b). Taken together, the peptide assays and overexpression data indicate that CLV2 and CRN are required for nematode CLE perception.

In order to serve as a receptor complex for nematode CLE peptides, CLV2 and CRN would most likely need to be expressed in feeding cell initials as well as the developing feeding sites. With the use of promoter-reporter lines we confirmed that both CLV2 and CRN were expressed in nematode-induced feeding sites (FIGS. 2 and 3), consistent with a role in nematode CLE perception. It is also possible that nematode CLE receptors are expressed in the cells adjacent to the expanding syncytium. As the nematode CLEs are redirected to the host root apoplast, extracellular receptors of the adjacent cells that are primed for incorporation could trigger plant CLE signaling pathways needed to fully form the syncytium. In the future it will be interesting to more precisely localize the CLV2 and CRN proteins within syncytia using immunofluorescence techniques. This will aid in determining whether or not these nematode CLE receptors are expressed within the cell wall openings that occur during syncytium formation or if they are expressed on the outer plasma membrane of the syncytium and/or adjacent cells.

Previous reports have demonstrated that SCN and BCN CLEs are important for nematode parasitism by showing a reduction in nematode infection after knocking down CLE expression in the worm using RNAi approaches (Bakhetia et al., 2007; Patel et al., 2008). To directly test for a role of CLV2/CRN in nematode CLE perception we performed infection assays on the receptor mutants.

We showed that a reduction in nematode infection occurs on the receptor mutants (FIGS. 4a and 6). Concurrently, we also saw a reduction in syncytium size in the receptor mutants (FIGS. 4b and 7). The fact that we saw a similar reduction in both nematode infection and syncytia size in both the single and double mutants is consistent with genetic and biochemical data that CLV2 and CRN are acting in the same pathway (Muller et al., 2008; Bleckmann et al., 2010; Zhu et al., 2010). These data indicate that not only is nematode CLE perception by CLV2 and CRN important for successful nematode infection, but demonstrates that CLE signaling also plays a role feeding cell formation.

The involvement of CRN in nematode CLE signaling also opens up the interesting possibility that nematode CLE signaling may be directly or indirectly suppressing host plant defense responses. It has been reported that in root tips of sol2-1, another mutant allele of CRN, plant disease resistance-related and stress responsive genes were upregulated (Miwa et al., 2008). Therefore, when nematode CLEs are secreted they could activate the CLV2/CRN signaling pathway leading to a suppression of plant disease resistance-related and plant stress responsive genes. One might speculate that the main target for nematode CLEs is a signaling pathway which allows developmental programming of root cells for syncytium formation to occur and that suppression of plant defense responses is just an added benefit to the nematode. Alternatively, the nematode may require suppression of plant defense responses through plant CLE signaling in order for the syncytium to form properly. Further studies will need to be performed to investigate this possibility.

Several possibilities exist for why we only see a partial reduction in nematode numbers and syncytia size in the clv2-1 and crn-1 mutant backgrounds. First, besides CLEs, nematodes secrete many different effectors that likely play an important role in feeding cell formation (Wang et al., 2001; Gao et al., 2003).

For example, when BCN CLEs were targeted with RNAi a similar partial reduction in nematode infection was observed (Patel et al., 2008), either as a consequence of limited reductions in transcript levels or an indication that the other effectors still active in the nematode allow infection to proceed. A second possibility for the partial reduction in the receptor mutants is that there could be multiple nematode CLE receptors. So far, the nematode CLEs reported belong to gene families (Lu et al., 2009; Wang et al., 2010a; Wang et al., 2010b). In addition, PCN CLEs have multiple CLE motifs that may be simultaneously processed to release different CLE peptides (Lu et al., 2009). This leaves the possibility that nematode CLE peptides may activate multiple plant CLE signaling pathways concurrently to function in an antagonistic or synergistic fashion as reported for plant CLEs (Whitford et al., 2008). The current plant CLV3 signaling pathway in the shoot indicates that there are parallel signaling pathways. Genetic evidence indicates that CLV1 acts in a separate pathway from the CLV2/CRN pathway (Muller et al., 2008). In support of the genetic data, recent reports using luciferase complementation assays and FRET analysis have shown that CLV1 forms a homodimer and that CLV2 and CRN form a heterodimer without CLV3 stimulation (Bleckmann et al., 2010; Zhu et al., 2010).

These reports also found evidence for CLV1 interacting with the CLV2/CRN complex leading to the possibility that different signaling pathways could be activated depending on which receptor in the complex interacts with the CLE ligand (Bleckmann et al., 2010; Zhu et al., 2010). Thus it is possible that in the crn-1 clv2-1 double mutants, nematodes are still able to signal through other receptors in the roots. Unlike CLV2, which has a broad expression pattern in plants, CLV1 expression is thought to be restricted to the center of the SAM and its function is thought to be confined to stem cell specification in the shoot (Clark et al., 1997; Fletcher et al., 1999). Therefore, in order to utilize CLV1 as a receptor, nematodes would have to activate CLV1 expression in the roots. Recently, CLV1-related Barely Any Meristem (BAM) 1 and BAM2 have been shown to act redundantly in the SAM and are widely expressed throughout the plant, including root tissues (DeYoung et al., 2006; Deyoung and Clark, 2008). We have found that bam1 is also resistant to exogenous application of synthetic nematode CLE peptides (A. Replogle, S. Chen, X. Wang and M. G. Mitchum, unpublished data). Moreover, there are over 200 LRR-RLKs in Arabidopsis and only a few receptor-CLE ligand pairs have been identified (Shiu and Bleecker, 2001). Thus, further studies using a combination of mutants will need to be performed to investigate the possible involvement of other host plant receptors in nematode CLE signaling.

It is shown here that nematode CLE signaling through the CLV2/CRN receptor complex is important for proper syncytium formation and ultimately successful nematode infection. These findings open the door for identifying the downstream signaling components regulated by CLV2/CRN to uncover the role nematode CLE signaling plays in syncytium formation.

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Example 2

Screening of plant CLE receptor mutants for resistance to nematode CLE peptides, overexpression of the nematode CLEs in the receptor mutant background, and infection assays of plant receptor mutants, has identified several receptors involved in nematode CLE peptide signaling. Plant receptor mutants exhibiting resistance to exogenous treatment of nematode CLE peptides include CLAVATA2 (CLV2; At1g65380), CORYNE (CRN; At5g13290), BARELY ANY MERISTEM (BAM1; At5g65700), and ERECTA-LIKE2 (ERL2; (At5g07180) (FIGS. 8 and 9). Overexpression of nematode CLEs in the clv2 and crn mutant background abolished all phenotypes (Table 2) that are observed when nematode CLEs are overexpressed in wild type plants (Wang et al., 2005; 2010: Lu et al., 2009). Additionally, nematode infection is significantly reduced on several of the receptor mutants including clv1, clv2, and crn (FIG. 10). Expression of receptors in nematode feeding cells was confirmed by infection of transgenic plants containing promoter-reporter fusions (FIGS. 11-13) and upregulation of candidate soybean and potato receptor genes in H. glycines-induced syncytia and G. rostochiensis-infected potato roots were revealed by microarray analysis of laser-captured syncytia (Table 2, 5% FDR; Ithal et al., 2007) and qRT-PCR analysis (FIG. 14). Thus, the disruption or modulation of the host plant receptor proteins that perceive the nematode CLE peptides can be used to develop a novel management tactic to reduce cyst nematode parasitism in crop plants including, but not limited to potato or soybean. Nematode control can thus be obtained by inhibiting receptor proteins that interact with any nematode CLE peptide. Endogenous plant gene encoding a receptor for a nematode CLE peptide that can be inhibited to provide include functional or structural orthologs of the receptor proteins in any plant species, including but not limited to receptor genes from monocot or dicot plant, or receptor genes selected from the group consisting of a tobacco, cereal, sugar beet, cotton, fruit, fiber, oilseed, potato, rice, corn, soybean, vegetable, and wheat plant.

TABLE 2 T1 Shoot Phenotypes Background Construct wus (%) PNC (%) WT (%) Total (#) Col-0 HgCLE2^(ΔSP) 78 6 15 80 HsCLE1 30 7 63 156 HsCLE2 6 5 89 158 clv2-1 HgCLE2^(ΔSP) 0 0 100 96 HsCLE1 0 0 100 67 HsCLE2 0 0 100 28 crn-1 HgCLE2^(ΔSP) 0 0 100 85 HsCLE1 0 0 100 41 HsCLE2 0 0 100 37

TABLE 3 Putative Soybean Receptors Upregulated in Soybean Cyst Nematode Induced Syncytia (5% FDR, LCM W82) Sequences corresponding to the genes provided below can be obtained from the world wide web (internet) using the identifiers provided in Table 3 from the following internet locations: 1) “the world wide web at soybase.org” or the world wide web at soybase.org/gbrowse/cgi-bin/gbrowse/gmax1.01/ 2) “the world wide web at phytozome.net” or the world wide web at phytozome.net/cgi-bin/gbrowse/soybean/?name=Gm09 3) “the world wide web at plantgdb.org” or the world wide web at plantgdb.org/GmGDB/(Assembly version Glyrna1.170 (April 2009) 4) the world wide web at ncbi.nlm.nih.gov/sites/entrez Affymetrix Probset Qvalue FC dpl 2 Soybean Gene Best At Hit E-value PFAM Description(30) Putative Soybean Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) Upregulated Gma.1778.1.S1_at 0.000696 11.19428 Glyma08g16220.1 AT3G12610.1 1E−100 Leucine Rich Repeat GmaAffx.78459.1.S1_at 0.000947 15.6836 Glyma18g42700.1 AT4G08850.1 0 Leucine rich repeat N-terminal domain Gma.17727.3.A1_at 0.001475 1.696212 Glyma13g37580.1 AT4G03390.1 0 Leucine rich repeat N-terminal domain Gma.9956.1.S1_at 0.001606 5.057105 Glyma15g26790.1 AT5G06860.1 1E−100 Leucine rich repeat N-terminal domain GmaAffx.3809.1_S1_at 0.001904 1.789864 Glyma18g00610.1 AT3G23750.1 0 Leucine rich repeat N-terminal domain GmaAffx.50347.1.S1_at 0.002786 26.80867 Glyma16g06940.1 AT4G08850.1 0 Leucine rich repeat N-terminal domain GmaAffx.91749.1.S1_s_at 0.003961 12.73998 Glyma15g26790.1 AT5G06860.1 1E−100 Leucine rich repeat N-terminal domain GmaAffx.49400.1.S1_at 0.006842 2.043868 Glyma07g31970.1 AT1G28340.1 0 Leucine rich repeat N-terminal domain Gma.17727.1.A1_at 0.007366 2.014757 Glyma13g37580.1 AT4G03390.1 0 Leucine rich repeat N-terminal domain GmaAffx.47891.1.S1_s_at 0.007905 8.874029 Glyma19g32700.1 AT5G06860.1 1E−112 Leucine rich repeat N-terminal domain GmaAffx.68769.1.S1_at 0.008658 4.20926 Glyma20g25570.1 AT2G01210.1 0 Leucine rich repeat N-terminal domain GmaAffx.24529.1.S1_at 0.008905 32.2986 Glyma12g00960.1 AT4G08850.1 0 Leucine rich repeat N-terminal domain GmaAffx.5435.1.A1_at 0.009418 1.368383 Glyma09g13540.1 AT5G51350.1 0 Leucine rich repeat N-terminal domain GmaAffx.65770.1.S1_at 0.012282 1.967988 Glyma18g06670.1 AT3G56050.2 1E−115 Leucine Rich Repeat Gma.9483.1.A1_at 0.016684 2.107688 Glyma19g29370.1 AT3G03770.1 0 Leucine Rich Repeat Gma.16818.1.S1_s_at 0.017963 2.874609 Glyma11g04700.1 AT5G65700.1 0 Leucine rich repeat N-terminal domain (BAM1) GmaAffx.6107.2.A1_at 0.018015 14.17985 Glyma06g13970.1 AT3G47570.1 0 Leucine rich repeat N-terminal domain GmaAffx.51790.1.S1_at 0.023845 3.00325 Glyma17g18520.1 AT5G67200.1 0 Leucine rich repeat N-terminal domain Gma.405.1.A1_at 0.026584 16.80313 Glyma11g13970.1 AT3G20820.1 1E−125 Leucine rich repeat N-terminal domain GmaAffx.24583.1.S1_at 0.02776 1.951831 Glyma05g29150.1 AT4G18640.1 1E−169 Leucine rich repeat N-terminal domain Gma.16642.1.S1_at 0.034358 1.219904 Glyma12g03370.1 AT1G10850.1 0 Leucine rich repeat N-terminal domain Gma.4557.1.S1_at 0.040772 1.199444 Glyma08g01640.1 AT1G63430.1 0 Leucine Rich Repeat Gma.5232.1.A1_at 0.044053 1.576101 Glyma06g36230.1 AT5G53890.1 0 Leucine rich repeat N-terminal domain GmaAffx.18359.1.S1_at 0.047925 4.386757 Glyma03g32270.1 AT1G35710.1 0 Leucine rich repeat N-terminal domain Gma.2806.1.S1_at 0.047969 1.0518 Glyma05g29530.1 AT1G29750.2 0 Leucine Rich Repeat Gma.15907.1.A1_at 0.048874 1.454241 Glyma18g53970.1 AT2G33490.1 1E−164 Leucine rich repeat N-terminal domain Other Putative Soybean Receptor-Like Kinases Upregulated Gma.17579.1.S1_at 0.001029 2.926118 Glyma06g07170.1 AT4G32300.1 0 D-mannose binding lectin|Protein kinase Gma.10903.1.A1_at 0.001984 3.921658 Glyma06g06620.1 AT5G54590.2 1E−101 Protein kinase domain GmaAffx.15130.1.A1_at 0.002275 8.229938 Glyma20g27740.1 AT4G05200.1 0 DUF26|Protein kinase GmaAffx.46387.1.S1_at 0.002957 1.854171 Glyma18g12830.1 AT3G59110.1 0 Protein kinase domain Gma.11892.1.S1_at 0.003085 2.491472 Glyma18g47170.1 AT1G01540.2 0 Protein kinase domain Gma.13100.2.A1_at 0.003827 2.576388 Glyma16g03650.1 AT1G01540.2 0 Protein kinase domain GmaAffx.4463.1.A1_at 0.004197 50.44552 Glyma10g39910.1 AT4G38830.1 0 DUF26|Protein kinase GmaAffx.8689.1.S1_at 0.004457 6.746447 Glyma06g02000.1 AT1G20650.1 1E−134 Protein kinase domain Gma.5637.1.S1_at 0.005376 3.595321 Glyma10g28610.1 AT2G47180.1 1E−150 Glycosyl transferase family 8 GmaAffx.37615.1.S1_at 0.006409 3.634731 Glyma17g06430.1 AT2G17220.2 1E−117 Protein kinase domain Gma.5416.1.S1_at 0.00667 1.151061 Glyma09g40650.1 AT5G01020.1 0 Protein kinase domain GmaAffx.65711.1.S1_at 0.007597 3.149387 Glyma18g45000.1 AT3G08600.1 5E−87 Protein of unknown function (DUF1191) Gma.8159.1.S1_at 0.007785 2.089075 Glyma14g03290.1 AT3G59110.1 0 Protein kinase domain GmaAffx.83551.1.S1_at 0.009487 36.43414 Glyma06g41150.1 AT4G21380.1 0 D-mannose binding lectin|Protein kinase GmaAffx.22464.1.A1_at 0.010743 2.290479 Glyma12g16650.1 AT5G54590.2 1E−176 Protein tyrosine kinase GmaAffx.56567.2.A1_at 0.010919 8.11891 Glyma10g05990.1 AT1G16670.1 6E−90 Protein kinase domain GmaAffx.60687.1.S1_at 0.014387 18.28579 Glyma08g46630.1 AT1G11300.1 1E−140 D-mannose binding lectin|PAN-like domain GmaAffx.8293.1.S1_at 0.015011 3.375224 Glyma08g22770.1 AT3G15890.1 1E−125 Protein kinase domain GmaAffx.76163.1.S1_at 0.0178 1.444014 Glyma09g32390.1 AT3G24550.1 0 Protein tyrosine kinase GmaAffx.87698.1.S1_at 0.020806 2.437942 Glyma12g11840.1 AT4G03390.1 1E−172 Protein tyrosine kinase GmaAffx.91247.1.S1_at 0.024984 2.593136 Glyma15g09490.1 AT4G18950.1 0 Ankyrin repeat|Protein kinase domain GmaAffx.10590.1.S1_s_at 0.026872 4.633997 Glyma06g40480.1 AT4G27290.1 0 D-mannose binding lectin|Protein kinase Gma.4931.1.S1_s_at 0.030153 1.894182 Glyma18g44830.1 AT3G51550.1 0 Protein tyrosine kinase Gma.7158.1.A1_at 0.032121 2.64156 Glyma12g08210.1 AT2G28250.2 1E−165 Protein kinase domain Gma.14610.1.A1_at 0.039013 4.911538 Glyma20g37470.1 AT3G05140.1 1E−153 Protein kinase domain Gma.3334.1.S1_at 0.042026 2.355478 Glyma02g43710.1 AT2G33580.1 1E−179 LysM domain|Protein kinase domain GmaAffx.93403.1.S1_at 0.042465 3.481746 Glyma05g25370.1 AT5G06870.1 7E−77 Leucine rich repeat N-terminal domain Gma.8195.2.S1_a_at 0.042848 2.222809 Glyma18g16060.1 AT2G02800.2 1E−171 Protein kinase domain GmaAffx.4480.1.S1_at 0.042861 6.312541 Glyma18g06610.1 AT5G58520.1 0 Protein tyrosine kinase Gma.8195.2.S1_at 0.045596 11.62797 Glyma18g16060.1 AT2G02800.2 1E−171 Protein kinase domain GmaAffx.19954.2.S1_at 0.045643 4.577217 Glyma10g43060.1 AT4G38470.1 0 ACT domain|Protein tyrosine kinase REFERENCES Gao, B. L., Allen, R., Maler, T., Davis, E. L., Baum, T. J., and Hussey, R. S. (2003) The parasitome of the phytonematode Heterodera glycines. Mol. Plant-Microbe Interact, 16, 720-726. Ithal N, Recknor J. Nettleton D, Maier T, Baum TJ, Mitchum MG. (2007) Developmental transcript profiling of cyst nematode feeding cells in soybean roots. Mol. Plant-Microbe Interact, 20(5): 510-525. Lu, S. W., Chen, S., Wang, J., Yu, H., Chronis, D., Mitchum, M. G., and Wang, X. (2009) Structural and functional diversity of CLAVATA3/ESR (CLE)-like genes from the potato cyst nematode Globodera rostochiensis. Mol. Plant-Microbe Interact, 22, 1128-1142. Mitchum, M. G., Wang, X. and Davis, E. L. (2008) Diverse and conserved roles of CLE peptides. Curr Opin Plant Biol, 11, 75-81. Patel, N., Hamamouch, N., Chunying, L., Hussey, R., Mitchum, M., Baum, T., Wang, X., and Davis, E. L. (2008) Similarity and functional analyses of expressed parasitism genes in Heterodera schachtii and Heterodera glycines. J Nematol, 40, 299-310. Wang, J., Lee, C., Replogle, A, Joshi, S., Korkin, D., Hussey, R. S., Baum, T. J., Davis, E. L., Wang, X., and Mitchum, M. G. (2010) Dual roles for the variable domain in protein trafficking and host-specific recognition of Heterodera glycines CLE effector proteins. New Phytol, dol: 10.1111/j.1469-8137.2010.03300.x Wang, J., Replogle, A., Hussey, R. S., Baum, T. J., Wang, X., Davis, E. L., and Mitchum, M. G. (2010) Identification of potential host plant mimics of CLV3/ESR (CLE)-like peptides from the plant-parasitic nematode Heterodera schachtil, Mol. Plant Pathol. (submitted). Wang, X. H., Allen, R., Ding, X. F., Goeliner, M., Maier, T., de Boer, J. M., Baum, T. J., Hussey, R. S., Davis, E. L. (2001) Signal peptide-selection of cDNA cloned directly from the esophageal gland cells of the soybean cyst nematode Heterodera glycines. Mol Plant-Microbe Interact, 14, 536-544.

Example 3 Promoter Sequences Useful in the Practice of the Invention

BARELY ANY MERISTEM (BAM1; At5g65700) - promoter sequence; SEQ ID NO: 3 tctcattaagcacctacttcccacatctttcttaaagtttcttacataaagctcccttcacacgtgcttaccaaatc agattgtcaataattcttgctcaataatttttcgaaatttatttgaatttatctaataaaaatacattgtttgagta tgatattttgcttaagaaggttgattattctccctatcaaagtctaaaaagaagattacaaaacaattgtatggtta aattcatataaatttgtgactagtattttaatatttacatatatacaaatacttatagatgaaacgagaatgcagaa atgattatagatagatcagtgacagtgaactgtagcaaccggcaaagaaacctcgttagctggacacacgattacga tcatgcccccagtctcctctgtccagacggctgcattaataacaacgagctagagggtgttttcgtcttttcgatac ttatcccaaaaccgacaatctctggtttggactcgaaggctgatttggtcaattcatagcaaccgaacgagcagtcc attcaagtccaaagagctccttagtggtaaaagatgtaattacgtagatgttccatggtcaagaatgtattcagtca aaataaatatttgaccaaaactttcggttaatttcctaccaccagcaaaattataactttttctaataattatcaat cattttcaatctcttttaattttctttttcacttttttttattaattaaagtcaattcacactatacaaaaagaagg aagtctaaatatttttttactttcatgttgcttttctaacttttatattttgctcttctcaacagattttgctggtt tttgtattagaaatattattatgtttccagaaatgaattttttatatgtcgtctggattcgtatatatatattggaa agtgaaattaattcatttgatttttttctttgatatatcgaccaaatcaaataaatacgaccccattgtggcattgt taatgcaaaaaggcacaagtacaaaaaaaacataataattcactattttatttacagacacatgggcccaattcata cggcccaattaccataaacctctcttttaaagagtgggttccacagtggtaaactttttgactatccattggaatga ttgcatctggaccgttcatctacattaattattgggttttttcgctttaaagcatcaattaacttattacgtatagg attagattaccaataacgatctttttagcttttgtcgttttccgataaaaccatacgattaagaatatgacctcttg tatcttttgagggattttagttaatctttctacatttattttgttggatgctcatacaattatcctgtgtctctcaa aataaaacaaaaattactctatttattagtacattacacatgattatttagaaaatgtatattgtggtcatatgaaa tgagaaattaaaggaaatttgtcaatacttgagaacatcaccattcaaatgtttcaagaacaacatgactccaaaac aaaataaatgaacctttccctaataatagtatattctccatcgtacaaagttctaaataatacaatattcatttcgt caaagcatatgatgtgttggaatcagaattatctgcaaatgtttgaatttcaaatgttagtatcaggctatttttac tgttttatcaaatatcgtttcttctgcaatctatcacttgattgttttatcaaatcagcactagtattattgatttt gtaatttgtgtttgtctacctccaattactttttagtgttatgattagtaatgtaataaaatcacaaatctgacgtg gcacctatatacaattccaaaaacaagtggaacgaatataaaacaaattcacaccttcctcatcttcttcttcgtct tcacttaccttctctctacactcacaccatctcacaaccctaatctctcccacacaagagagatagagagaaaca CLAVATA2 (CLV2; At1g65380) - promoter sequence; SEQ ID NO: 4 CACATACATagacacaaagccctttccattgtcctcttcgtttccttttgggtaaacaaccaatctcctgatttt tacaaaaaaggcaacatttcttagttatatatgcttgtagtgaagaaagatgtgaaagtctgacaagagaacaag acgaaggaggagtctttctccaagtcttcaacattgcagaatctgatgcatatgaacccattttctctacaaaat gttgcaaccctagagagcaaaacaaaacatacccataatcagaaatgatctgacgaaaatcgagttacaatacac aagagaacattttttttagaattctcagatattaaaaatgacacagaaagctttatgctttttcctettaaaaga ctaaacaagttgaaatctagagaaagaactgaccaacctgagacaacgagagagacttgagagatttcttcggca cttactattagatctagggtttagataccatttatatagagaaagttttagagttgcacaaaacataaattaatg tgttagaatgggcctaaagctacaaagctggcctggttttgttttaaattgttggtttcatggacattttcgaca tcttcgaacatgttattttttgagactatgcaaacttgaagctctttactcgagttgaaatcgtatgacttatag tgaaattgtacatttggtttcgatttttcttttacactctttcttctttgagccggtaaatttggaatttttctt catagtggaatcatatgctgttttttttttttatagtaaacgttacaagaatgaatggtaactttatccaaaaaa aaagaatcatattattttgaaatgattttaagtaaattctaggttcaataacataagatttgagactaaatttaa aatttcttagtaaaatatatgatttttttataaatacctataaaattagtaattaacaatacggattacgtactg aatcaaaccctttgtattttgtttttcctagaaataagtgtagatttttggaattttgcattaattaatcacttc ttgggtctgaaaggctaaaacaaaaggaaccgaaagagaatgttctctctgtctttatcttccacttccacttcc aggtcgcgttgcttcactctccattgcaaagagaggtctctgcgatttctgcaactcacccctgaaaccttctta atttacttcaactgccgctatacctaaaaacttcatctttctcctctgagctATGATAAAG CORYNE (CRN; At5g13290) - promoter sequence; SEQ ID NO: 5 aaagatgcataggcttgcggacataaaaattccggagctatg tttcatcgttgctttcacggtctgaagagccaatcaacactaaagaaggacctctaatgg tctctagcaagtttagcccccaattaagtattgtattgatgtttttgtgatggatggata taggctgcatattgggaaattatagtgtattgtattgtgtcgtgttgtgtgtatgtggga ctatagcatcctgagtttgtcatgtccagacgttgtaacttgtaagcaattacttatggt tttgttcacttcgtattaacgtatttaatttgtggctcgattttggttttgaatctgtgt caaaactaagataatttacgtgttaaaccaggcccaagtttgaaagttaattgtcaattt tcagaccagagtacatattggtccacttattcccattacattcatagttttgagtctttt gataatagtgttaccatttcaattaggctaatcttttttcaacccaagatatttttataa aaaggaatgtggttcaaatcggaaaacaagacctaactttgaataaaagcactacagcat aaagcttttacctttaacaaaaaaaatataataattttttacaaggaaaaagaagagaaa gcaattattctcagacaaacaaaggaaccacttttgtaggtgtagtagtaatctcacacg ctaagacaaaagtgcacaaattctcgagactctcttctatccaacggtccatatctcact aaccgcatctaaataacggacaagatcttcttttggcttcagctctctttagtctttacc ttccctcaagctcggtactcgatgtcttgctttcggccactcatgaaagcaacgagagct tcccctttcatccgcctacgtggctatgggacccagtctaaccacgaccacctgacatcg tgggccccactgtaaggcgggaaccccatttttttttggctgtaagtaacggattctcgg tcatgcttttttgtgaggatagagagagagactgagagagagagagagtgtgtcacggtc tcgcagatactgtgtattgaaaagagagttctagagagagagtgtgttatgtgtgtgtgt gtgtgtgtgtgtgtgtgtgtgtgtgtttggttactgggattaattgagctgaaacagttt ggatagttttgtttgttctgtttcatctttcaaccacagatatagtaatattgtgaaaac ccctcattgaagtttgttctctgctctctctttttgggtttagcactgagttttggggtt tatttcgagacatacccatacaaagtttgatacttttgtgtccccccttatcaagaaaat tgtggggtttttttttttttttaataagcttcctttaaattttcaatttttattttggag gaaaagagtgagaatttcagataagaatctatgagccaatgatattctaattcatcttct tcgtgaagattttgagttgaattccattttcctttttgtcttggtggtttctcattggtt ttctcgagaatatttgtggttttgggagaagaggcttcactgtagcattgaaaaagtctt aaacttttctgtgtctttttatgtaagctttgaacagcttcacctttctgggttttctca gattgtgtctaatcttgaaaaaccttttattcgtagaagcagca

Promoters associated with any of the potato genes provided below in Example 4 are also provided herewith. In particular, use of the promoter associated with the StCLV1 gene provided below in any of the methods of this invention is provided.

Example 4 Sequences of Various Candidate Potato Nematode CLE Receptor Genes are Provided

Sequences correspond to potato genes analyzed in FIG. 14 and as described in the claims and Example 2. The ATG start codon and TGA stop codon are underlined.

>StCRN cDNA (From clone 4-3: (SEQ ID NO: 6) ATCGCATGGTTTCATGGAGCTCCTTGTTTTTTTGTTGGAATTTGATGATTTTCCAATTTGGTTATTATGTTGTTC ATTGTTGTTGTTGAGTCTATTTTGTGGTGGTGCGGAGGTGTGAGCTTTAAATTGGAGTTGGGGTGATTGTTGTTT TGTTCGCCGGAGAAGCCATCTCCAGTGAGGTTGGTTGGAGAAGGAGAGAGATGAGGAGAGCAATGAGTAATTTCA ACTATTAAAGATTCGTTTCAGAAAGAGAAAAAAAGAAGAAAATGGTCACATTGTCGTCCTTGTGTAACATTCAGA GGAGTGAACCCTAAACTTGCCGACCCACAGAGAAAAACAACCCTAGTTTCCATGGGGACCTGCTGTAACAGTAGC ACAGTTCTCAAGCTTTGTTTTTTGTGGCTACAACTAATCTGTGTGCAATGCCATGGAAGGATACTCAAGGATGAT ACCTCCTCATCTGATCAGTTTAAGAACAGATTTCAAAGGATTTTTCTGAGTATACTTTTTGGTATGTTTACAGGA TTGATTTGTGCACTTGTTTTTGCTTGGCTTGTTCGGAGTTTTGTTCGTTACATTAACAAAGCCCCAATTCTCAAA GGCCCTGTTGTATTCTCTCCTAAAATTCCATCCAAAACTCTGCAATCAGCTCTTGCTAATGATACCCAGTTGATA GGGTCAAGTAGTTCTGGAAAATACTACAGAACTGTTCTTGATAATGGGCTTACTGTTGCAGTTAAGAGAATGGAA CCTGGTTCTCCACAGTTACATACCAAGTCATTTAAGAGAAGAATACAACACGAACTTGAACTTATTGCTAGTTTG AGGCATAGGAATTTGATGAGTTTAAGGGCTTATGTTCGTGAATCGAATACGTTCTTTCTGGTTTACGATTATGTA AACACTGGCAGTCTTGAAGATGTAATGAACAAAGTTAGGGAAAATCAATTGCAACTTACCTGGGAAGTCAGGCTC CGAATTGCAGTTGGGATTGTTAAGGCTCTTCAGTATCTTCATTTCTCTTGTAACCCCACAGTTTTGCATCGGAAT TTGAAACCCACAAATGTAATGTTGGATGCTGAGTTTGAGCCTAGGTTGGCTGATTGTGGTTTGGCTAAAATCATT CCCACTTTAAATCTCCCTGCTGCATCAAACTATGGTCCTCCAGAATCATTCCAGAGTTGCAGCAGGTATACCGAT AAAAGTGATGTATTTAGCTTTGGGGTTATATTGGGTGTTCTATTAACTGGAAAGTACCCAACAGATCCCTTCTTT GGGGATACATCTACTGGAGGAAGTCTAGCACGTTGGCTTCAACGCTTGCAGGAAGCAGGCGATGCTCGAGAAGCA TTGGATAAGAGTATTCTAGGGGAAGAGGTTGAGGAAGATGAGATGTTAATGGCAGTAAAAATAGCAGCGGTATGC TTATCAGACATGCCTGCTGATCGACCTTCCAGTGATGAGCTCGTTTCCATGCTCACCCAATTAAATAGCTTCTGA TTAATTACTTTGGTCGAGAGGGAAAGCAGTCAAGGATTCAAATAATCACAAGATCTTTAAGGTTGTTCTTTTGGC TTTCTAAGGTGATAGTTTGCTGTGTGCTTTTGGTAGTTGAGCAATGCCTTTTGGTTATCGCAATGAGCACGAGTG TAGTTGGC >StBam1 (SEQ ID NO: 7) TTCTCACTCTCACTGAGTGAATCTGCAAACCAAACAGTTGGTGGGCATTAGATTAAGGAAGGAAAAATGCGTCTT CTTTTTCTTCTTCTTCTTGTTATGCATTTTACTGACTTTTCCGCCGGTAAACAACCTCGGTTACCGGAATATCAG GCTTTGCTTGCCCTGAAAACTGCCATTACCGATGACCCACAGTTAACACTTGCCTCATGGAACATCTCCACCAGT CACTGTACGTGGAATGGTGTCACGTGCGACACGCATCGTCACGTGACCTCTCTTGATATTTCTGGGTTTAATCTT ACCGGTACTCTTCCGCCGGAAGTTGGGAATCTTCGTTTCTTACAAAATCTGTCTGTTGCTGTTAACCAGTTTACT GGACCCATTCCTGTTGAAATCTCCTTTATTCCAAATCTCGGTTACCTTAATCTTTCTAATAACATATTCGGGATG GAATTCCCTCCGCAGTTAACCCGTCTGCGTAACCTCCAAGTCCTTGACCTTTACAACAACAATATGACCGGTGAA CTTCCCCTTGAGGTGTATCAGATGACTAACCTTCGACATCTACACCTCGGCGGGAACTTTTTCGGTGGCCGCATT CCTCCGGAGTATGGAAGGTTCCCGTCTCTAGAGTACCTCGCAGTTTCAGGCAATGCACTGGTAGGAGAGATACCA CCGGAGATTGGAAACATCACTACACTTCAGCAGTTGTATGTAGGATACTACAATACCTTCACCGGTGGGATTCCC CCGGCAATAGGGAACTTATCGCAGCTCCTCCGGTTTGATGCTGCTAACTGTGGACTTTCGGGGGAGATTCCACCG GAGATTGGGAAGCTTCAGAACCTTGACACTCTCTTCCTGCAAGTGAATTCTCTGTCTGGGTCATTAACTCCGGAG ATAGGTTATCTGAAGAGCTTGAAATCTTTGGATCTGTCGAATAACATGTTCTCTGGCGAGATACCGCCAACATTT GCGGAGCTTAAGAATATCACTCTTGTTAATCTTTTTCGGAATAAGCTTTATGGGTCAATACCAGAGTTCATAGAG GACTTGCCGGAGCTAGAGGTGTTGCAACTTTGGGAAAATAACTTTACGGGAAGCATTCCACAGGGGTTAGGCACA AAGAGCAAGCTCAAAAATGTTGATCTCAGTTCCAATAAATTGACTGGAAATTTACCCCCAAACATGTGTTCCGGT AACAATCTGCAGACAATTATCACTCTAGGGAACTTCTTGTTTGGCCCAATTCCTGAATCTTTGGGTAGGTGTGAA TCACTTAATCGGATTAGGATGGGAGAGAATTATCTGAATGGGTCAATTCCAAAGGGGCTCTTAAGCTTGCCACGT CTGTCACAAATTGAACTTCAGAATAATATTCTCACTGGTACATTTCCTGATATTTCTTCCAAATCTAATAGTCTT GGGCAGATTATCCTTTCAAATAATCGCCTAACTGGACCTTTGCCGCCAAGCATTGGAAACTTTGCTGTAGCCCAA AAATTGCTTCTTGATGGGAACAAATTTTCGGGACGAATTCCAGCAGAAATAGGAAAGCTTCAACAGCTATCCAAA ATTGATTTCAGTCACAACAACTTTTCTGGACCCATGGCTCCGGAGATTAGCCAGTGCAAGTTGCTGACTTATGTT GATCTCAGCAGGAACCAACTTTCGGGTGAGATTCCTTCTGAGATCACAGGTATGAGGATACTCAACTACTTGAAC TTATCGAGAAACCACTTAGTTGGGAGTATTCCTTCCCCTATTTCTAGTATGCAGAGTTTAACTTCTGTTGATTTC TCATATAACAACTTTTCTGGTTTAGTTCCTGGAACCGGGCAATTTAGTTATTTCAACTACACCTCATTTCTGGGC AATCCAGATCTTTGCGGACCCTATTTGGGCCCTTGCAAAGAGGGTGTTGTTGATGGGGTTAGTCAACCTCATCAA CGAGGAGCCTTATCGCCTTCGATGAAGCTTTTACTTGTTATTGGTTTGCTTGTCTGTTCTATTGTGTTTGCTGTT GCTGCAATTATAAAGGCCCGATCTTTAAAGAAGGCAAGTGAAGCTCGTGCCTGGAAGCTCACTGCTTTTCAGCGC CTAGATTTTACTTGTGATGATATTTTGGACAGCTTGAAGGAGGATAACATTATTGGAAAAGGAGGTGCTGGTATA GTCTACAAGGGGGTAATGCCGAGCGGGGAACATGTAGCAGTTAAGAGGTTGCCAGCTATGAGCAGGGGTTCCTCT CATGATCATGGGTTCAATGCAGAGATACAGACTCTTGGGAGGATCCGACACAGGCACATTGTTAGATTATTAGGA TTTTGCTCGAATCATGAGACAAATCTTTTGGTTTATGAGTACATGCCTAATGGAAGTCTTGGGGAAATGCTTCAT GGCAAGAAAGGCGGTCATCTACATTGGGATACCAGGTATAAGATAGCCGTGGAGTCTGCAAAGGGTCTTTGCTAT CTCCATCACGATTGCTCTCCTTTGATCCTCCATCGTGATGTGAAATCAAACAACATTCTGCTAGACTCCAGCTTT GAAGCTCATGTTGCTGATTTTGGACTTGCTAAATTCTTGCAAGATTCAGGGACATCAGAATGCATGTCTGCTATT GCTGGTTCTTATGGGTACATTGCTCCAGAATATGCTTACACGCTTAAGGTTGATGAGAAAAGTGATGTATATAGC TTCGGTGTGGTGCTATTAGAACTGGTAAGTGGCAAAAAGCCAGTTGGAGAATTTGGTGATGGTGTTGACATAGTC CAATGGGTTAGGAAAATGACTGATGGGAAAAAGGATGGAGTTCTCAAGATCCTTGACCCAAGACTCTCAACGGTT CCCCTTAATGAGGTGATGCATGTCTTCTATGTCGCATTGTTGTGTGTTGAAGAGCAGGCTGTGGAACGCCCCACC ATGCGA > StBam2 (from clone 6-4;) (SEQ ID NO: 8) CCACCATTGAAGAAACATGCGTTTTCTTCTCCTCTTCTTCCTTTCCCTTATTCTCCATTTCCATCTCCTCCACTT CACCACCGCAAAACCACCTTACGTGCCAGAATACCGGGCATTACTCTCCCTGAAAACTGCCATTACCGATGACCC ACAATCTGCTCTTCTTTCATGGAATATCTCAACAAGTCATTGTACATGGAGAGGTGTCACGTGCGACCGGTATCG TCACGTGACTTCTCTCGACATCTCTGGTTTTAATCTCACCGGTACTCTCACGCCGGAAGTTGGTCATCTCCGTTT TTTGCTCAATCTTTCTGTAGCTGTTAACCAGTTCTCTGGACCCATTCCTATAGAGCTCTCGTTTATACCAAATCT GAGTTACCTTAACCTCTCTAACAACATTTTCAATTTGAGTTTCCCTCCCCAGCTTACCCATCTCCGGTACTTGAA AGTTCTCGATATTTATAATAACAATATGACCGGTGACCTTCCGGTTGGGGTTTACAATTTGACTAATCTTCGACA TCTTCATTTGGGTGGCAATTTTTTTAGTGGCAGTATTCCACCGGAGTATGGTAGATTCCCATTCCTAGAATACCT TGCAGTTTCTGGAAATGCGCTCGTCGGTATGATACCACCGGAGATCGGAAATATTACCACACTTCGTGAGCTTTA CATTGGATACTACAACACGTTTTCCGGTGGGTTACCGGCGGAAATAGGGAACTTGTCGGAGCTCATTCGGTTAGA TGCTGCAAACTGTGGACTTTCCGGTGGGATTCCGCCGGAGATAGGGAAGCTTCAGAAATTAGATACACTGTTCTT GCAAGTGAATGGTCTTTCTGGGTCTGTTACACCGGAATTGGGGAATTTAAAAAGCTTGAAATCTTTAGATCTATC AAACAATATGCTCTCCGGTGAAATACCGTTCACATTCACAGAGCTGAAGAATCTAACTCTGCTAAATCTTTTCCG TAACAAGCTTTACGGGTCGATACCGGAGTTCATAGAAAATTTGCCGAAACTGGAAGTATTGCAGCTTTGGGAAAA CAACTTTACCGGAAGTATTCCACAAGGTTTAGGCAAAAACAGTAAGTTAACAAACGTTGACATCAGTACCGACAA ATTAACCGGAAATTTGCCCCCAAACATGTGTTCCGGCAACAAGTTACAGACGTTGATCACTCTTGGAAACTTCTT GTTTGGCCCAATTCCAGAATCTTTAGGTGAGTGTCAATCACTTAATAGGATTAGAATGGGAGAAAATTTTCTAAA TGGGTCTATTCCAAAAGGGCTATTCAGTTTGCCCAAGCTTTCACAAGTAGAACTTCAAGATAATCTTCTCACTGG TACATTTCCAGTGACTGGTTCTGTTTCATCAAGTCTTGGACAGATTTGTCTGTCGAATAATCGTTTCACGGGGCC TTTGCCATCGAGCATTGGAAATTTGACTGGTGTTCAAAAGTTGCTTCTTGATGGGAACAAGTTTTCTGGTCAAAT TCCAGCTGAATTAGGGAAATTGCAGCAGCTGTCGAAAATGGATTTTAGTGGTAACAGTTTTTCAGGCCTGATTCC ACCGGAGATAAGCCAGTGCAAGGCTTTAACTTATGTTGATCTTAGTAGGAATAAGCTATCTGGTGAAGTTCCTAC TGAGATCACTGGTATGAGGATACTGAATTACTTGAATGTATCGCGGAATCAGTTAGTTGGGAGTATTCCTGCACC TATTGCAGCAATGCAGAGTTTAACCTCGGTTGATTTTTCGTATAACAACTTATCTGGATTGGTTCCGGGTACTGG TCAGTTCAGTTACTTCAATTACACATCATTTATTGGTAATCCAGATCTTTGCGGACCCTATTTGGGTCCTTGCAA AGAAGGTATTGTTGATGGGGTTAGTCGACCTCATGAGAGAGGTGCATTTTCGCCTTCTATGAAGCTTTTACTTGT TATCGGGTTGCTTGTTTGCTCGATTGTGTTTGCTATCGCTGCAATTATTAAGGCTAGATCGTTAAAGAAGGCGAG TCAGGCTCGTGCCTGGAAGCTTACTGCTTTCCAACGCCTGGATTTCACTTGTGATGATGTATTGGAATGTTTGAA AGAGGATAACATTATTGGTAAAGGAGGTGCTGGAATAGTATACAAGGGGGTAATGCCAAATGGTGAACTTGTTGC TGTTAAAAGGTTGCCGGTTATGAGCCGTGGTTCTTCCCATGATCACGGGTTTAATGCCGAGATACAGACACTTGG GAGTATTCGACATAGACATATTGTTAGATTATTAGGATTTTGCTCAAATCATGAAACAAATCTTTTGGTTTATGA GTACATGCCTAATGGGAGCCTTGGTGAAATGCTTCATGGAAAGAAAGGAGGTCACTTGCATTGGGATACCAGGCA TAAGATAGCATTGGAGGCTGCAAAGGGACTTTGTTATCTTCATCACGATTGCTCGCCTTTGATCCTCCATCGTGA TGTAAAATCAAACAACATTCTTCTGGATTCCAGCTTCGAAGCTCACGTTGCTGATTTTGGGCTTGCCAAGTTTTT GCAAGACTCGGGAACATCAGAATGCATGTCTGCAATTGCTGGTTCTTATGGCTACATTGCACCAGAATATGCATA CACACTCAAGGTAGATGAGAAGAGTGATGTATACAGCTTTGGTGTGGTTCTGTTAGAATTGGTGAGCGGGAAAAA GCCAGTTGGGGAATTTGGTGATGGCGTTGACATAGTCCAATGGGTAAGGAGGATGACCGATGGGAAAAAAGAAGG AGTTCTAAAGATCCTTGATCCAAGACTCTCAACAGTTCCCCTTCATGAGGTGATGCATGTGTTCTATGTTGCAAT GCTGTGTGTCGAAGAGCAAGCTGTTGAACGCCCCAAAATGCGTGAGGTTGTGCAAATGCTAACTGAGCTTCCCAA GCCATCTGGTCCAAAAACAGAAGATTCAACAATCACCGAGTCGCCCCCATCATCAGGTCCTGCATTAGAGTCTCC CACTTCGACTCCCGGAGACACGAAAGACCAGTACCACCATCAGCCATCACCTCAATCTCCTCCACCTGACCTACT CAGCATATGACCTACAATGTTCCCTTCTAATAGAGGATG >StER (From 8-16; (SEQ ID NO: 9) GTCGGTAAGTCCAAGAACTGGTTTTTCAATTCAAAGGAGCTGAGTTAGTGTAAACACTTTTGGTTTTGAGTTTTG ACAGAGACTTGAGTCTCAGAGAAACTACCATGGCATCATTTTTACTTCAAAGATGTAATCTTTTCTTTGAGGTTC TTCTTCTTTTGGGGTTCTTGATTTTCTTCAGCTTTGGTTCTGTGGTGTCTGATGATGGTTCTGCATTGTTGGAGA TTAAGAAGTCAATTAGGGACATGGAGAATGTGTTGTATGACTGGACTGATTCTCCTTCATCTGATTACTGTGCCT GGAGAGGTGTTACCTGTGATAATGTCACCTTCAATGTTGTTCAACTTAATCTTTCGAGTTTAAATCTTGATGGGG AGTTGTCTCCTGCAATTGGACAGCTCAAAGGCCTTATATCTATTGATGTTAGGGGAAATCGCCTTTCTGGCCAGA TACCAGATGAGATTGGTGACTGTTCAGCACTGAAAAACTTGGACCTATCCTTCAATGAGCTTTATGGTGATATTC CGTTTTCCATATCAAAACTTAAGCAACTGGAATATCTGATTATAAAGAACAATCAGTTGATTGGACCAATTCCAT CGACATTGTCACAGATCCCCAACTTGAAGGTCTTGGACCTGGCTCAAAATAGGTTAAGTGGAGAAATTCCTAGGC TGATATACTGGAATGGAGTCCTGCAGTATTTGGGACTGCGTGGCAACAACTTGGGTGGATCACTTTCTCCTGATA TGTGTCAGCTCACCGGCCTGTGGTACTTTGATGTTCGGAACAATAGTTTGACTGGTTCCATTCCTCAAAATATTG GCAACTGTACTGCTTTCCAGGTTCTAGATTTGTCTTATAATGACTTGACTGGAGAGATTCCTTTCAACATTGGTT TCCTGCAAGTAGCGACCTTGTCTTTGCAAGGTAATCGCCTTTCAGGGCAGATCCCTTCTGTCATTGGATTGATGC AAGCTCTTGCAGTTTTGGACTTGAGCTGCAATATGTTGAGTGGAACAATTCCTTCAATTCTTGGGAATTTGACTT ACACAGAAAAATTGTATCTACATGGGAACAAGCTATCTGGTTCCATTCCTCCAGAGCTGGGAAATATGACAAAGC TTCACTACTTAGAATTGAATGATAACCAACTTACTGGACGCATACCACCAGAACTTGGAAAGCTGACGGAGTTGT TTGACTTAAATGCTGCAAACAACCACCTTGATGGGCCCATTCCTTCCAATCTTAGCTCATGTACCAATTTGAATA GTCTCAACGTTCATGGAAACAAATTGAATGGTACGATTCCACCTGCTTTTCAAAAGCTGGAAAGTATGACCTATC TTAATCTCTCCTCCAACAACCTCAAAGGCCCAATTCCAATTGAGCTTTCTCGTATTGGGAATGTAGATACACTGG ACTTGTCAAACAACAGGATCAGTGGTCCTATACCTTTGTCCCTCGGTGATTTGGAACATCTTCTTAAACTGAACT TGAGCAAGAACGAAATAAATGGAAACTTGCCAGCTAAATTTGGCAATTTAAGGAGCATCATGGAGATTGATCTGT CAAGCAATCACCTCTCTGGTCCCTTGCCTCAGGAACTTGGTCAGCTTCCAAATCTGTACTTGCTGAAACTGGAAA ACAACAATTTATCAGGCGATGTGATGTCCTTAGCCAGTTGTCTCAGTCTAAATGTCCTAAATGTCTCGTACAATA ATCTGGGAGGGAATATTCCAACAGGCAATAATTTCTCTAGATTTTCACCAGACAGCTTCATAGGAAATCCAGATC TGTGTGGGTATTGGCTCACTTCTCCTTGTCATGCATCTCATCCAGCAGAGCGAGTTTCAATTTCTAAAGCTGCTA TACTTGGTATTGCTCTGGGTGGCTCGGTGATTCTTCTGATGATACTAGTAGCAGCATGCCGGCCACAGAATCCTG CACCTTTCATGGAAGGATCTATTGATAAACCAGTTTATTACTCATCTCCAAAGCTTGTGATCCTTCATATGAACA TGGCACTTCATGTTTACGAGGACATTATGAGGATGACTGAGAACTTGAGTGAGAAGTATATAATTGGTTGTGGAG CATCAAGTACGGTATATAAATGTGTTTTGAAAAATTGCAAGCCTGTAGCTATCAAGAAATTGTACTCTCACAACC CGCAATACTTGAAGGAATTTGAGACTGAACTTGAGACAGTTGGGAGTATTAAGCATCGTAATCTTGTCTGCCTCC AAGGATATTCTCTTTCTCCATCTGGCCATCTTCTTTTCTATGACTACATGGAAAATGGTAGCCTTTGGGATTTGC TTCATGGTCCTACAACAAAGAAGAAAAAGCTTGATTGGGTTACTCGCCTTCGAATTGCATTGGGATCAGCTCAAG GGCTTGCATATCTTCACCATGATTGTAGCCCTCGAATTATCCACCGTGATGTTAAATCATCAAATATCTTGTTGG ACAAAGACTTTGAGGCTCATCTGACTGATTTTGGCATTGCCAAAAGCTTATGCATATCAAAGACCTATACGTCCA CATACATTATGGGAACCATTGGTTACATTGATCCAGAGTATGCTCGCACTTCTCGCTTGACAGAGAAGTCTGATG TTTACAGCTATGGAATTGTTCTATTGGAATTGCTCACTGGAAGGAAAGCTGTAGATAATGAGTCTAATCTACACC ATATGATTCTAACTAAGGTAGCAAACAATGCTGTAATGGAAACAGTGGATCCTGAGATCACAGGCACATGCAAAG ATCTTGCAGATGTGAAGAAGGTTTTTCAGCTTGCCCTTCTATGTTCCAAAAGACAGCCTGCTGAGAGACCAACAA TGCATGAAGTGGCAAGAGTACTTGAAAGCCTAATACCCGTCACTGAAATGAAACAGCCAAATCCAACGCTCTCAC TTGCATTACTTCCATCTGCTAAGGTACCTTGTTACATGGATGAATATGTCAACCTCAAGACACCCCATCTAGTGA ATTGTTCATCCATGAGCATTTCAGATGCTCAACTTTTCCTGAAGTTTGGAGAGGTCATATCCCAGAATAGTGGCT GAAAATAACATGAGTAGATTTCTTGGGATTGTGTAAAAAAATGTAGTGCCATTATAATATTATTATTGTAGGTAG TTGTTGTAAGATGATGCATGCAATAGTGGTCCAGTCTACTTTTTCCACTACATAGGTCTAGTGTGTGTAAAAATA TTTCACTTTTTACCATGATGAAATTGGAAGAGGTAGCACTTGGTAGAGTATTGTAATATTGGTTTTTGGGACTGA TGCTGAGTATGGACTATACTGTCTGTAGGATTTTTGGCACACACTTTGAGGTGGCCTTAGCA >StCLV1 (From clv1 clone 11-1 041710; (SEQ ID NO: 10) AGACTAAACTAACAGTGTAATAATGTCACTCCCCAAAAAAATATCCCTTTTCCTCCAAATTTTCATTTTTTTTGT TTTCTCCATTAATGCAAACTCTGATCTTGAAACCCTTTTGAAGCTCAAAGAATCCATGGTTGCTCCTGGAACTTC TGCACTTCTTGATTGGAACAACAACACAAATTACCCTTTTTCCCATTGTTCTTTTTCTGGTGTTACATGTAACAA TAACCCTCATGTTATATCTATAAACATCACTAATGTTCCTCTATTTGGTACTATTCCACCTGAAATTGGTCTTTT ACAAAATCTTGAAAATCTTATTATTTTTGGTGATAATATTACTGGTACACTCCCTTTAGAAATGTCACAACTTTC TTCTATTAAACATGTTAATCTTTCTTACAACAACTTTTCTGGTCCTTTTCCTAGAGAAATCTTGTTGGGGTTAAT AAAGCTTGAATCTTTTGACATTTATAACAACAATTTCACTGGTGAACTTCCTACTGAGTTTGTAAAGTTGAAAAA GTTGGAAACTTTACATCTTGGTGGAAACTATTTTCATGGTGAAATACCAGAAGTTTATTCTCATATTGTAAGTTT AAAGTGGTTGGGTTTAGAGGGAAATTCACTAACTGGGAAAATACCAAAGAGTTTGGTTTTGTTACCAAATCTTGA AGAACTTAGATTGGGCTATTATAATAGTTATGAAGGGGGTATTCCATCTGAGTTTGGTAATATTAGTACACTTAA ACTTCTTGATCTTGGAAATTGTAATCTTGATGGTGAAGTTCCTCCAAGTCTTGGAAATTTGAAGAAGTTGCATAC TTTGTTTCTACAAGTGAACAGACTTACAGGTCGCATACCTTCTGAACTATCTGGTTTAGAGAGTTTGATGTCGTT TGATTTGTCTTTTAATCAACTGACCGGAGAAATACCAGAGAGTTTTGTGAAGTTGCAGAATTTGACATTGATTAA CTTGTTTAGAAACAACTTGCATGGTCCAATTCCCCCTTTTATTGGTGACCTTCCAAATCTTGAAGTGTTGCAGAT TTGGGGAAACAATTTTACTCTTGAATTGCCCGAAAATCTTGGGCGTAACGGGAGGTTTTTGTTTCTTGATATTTC TATTAATCATTTTACTGGAAGGATACCACCTGATTTGTGTAGAGGAGGGAAGTTAAAGACACTGATTCTAATGGA AAATTACTTCTTTGGTCCAATTCCTGAACAACTTGGTGAGTGCAAATCGCTTGCTCGAATTCGCGTTAGGAAGAA TTACTTAAATGGTACTATTCCAGCTGGTTTTTTCAAGTTACCTGCATTGGATATGCTTGAACTTGACAACAACTA TTTCACTGGTGAGCTGCCAACGGAGATAAACGCGAATAATCTCACTAAACTTGTACTTTCCAACAACTGGATCAC GGGGAACATTCCTCCATCATTAGGGAACTTGAAGAATCTAGTCACTCTATCACTTGATATGAACAGGTTATCTGG TGAAATTCCTCAAGAAATTGCGAGTTTGAATAAACTCGTGACCATCAACTTGAGTGGCAACAATTTAACAGGTGA AATCCCAAGTTCAATTGCGCTTTGTTCAGAGCTAACATTGGTTGACTTGAGCAGAAACCAACTGGTTGGTGAAGT GCCAAAAGAAATCACCAAGTTAAATAGCTTGAACGCTCTGAACTTGTCAAGAAACCAACTGAGTGGCGCCATTCC TGGAGAAGTCGGAGTGATGAATGGCTTGACAGTTTTAGATCTTTCTTACAATGATCTTTCTGGAAGGAGACCGAC CAACGGACAACTAAAGTTCTTCAATGACACTTATTTTGTAGGAAATCCAAAACTCTGTTCACCTCATGCTACTTT TTGCCCGTCAGCCTCCAATTCACCACAAAACGCGCTCAAAATCCATGCTGGGAAGTTCACAACTATCCAATTGGT GATTACAATAATCATCTTAGTCACTGTTGCATTGCTGTTGGCAGTTACCGTGTTGTTCATCAAGAAGGAAAAGTT CAAGAATTCGAAACTTTGGAAGTTAACAGCATTCCAGAAACTTGATTTCAGAGCTGAGGATGTTTTGGAGTGTTT AAAAGAGGAGAACATAATTGGGAAAGGTGGAGCTGGCGTTGTGTACCGAGGGTCTATGTCAAATGGCATCGACGT TGCAATTAAGAAACTTGTAGGCCGAGGAACTGGACACCATGATCATGGATTCTCAGCTGAAATCCAAACACTAGG AAGGATCAGGCACAGAAACATCGTACGATTACTAGGATATGTCTCAAACAAAGACACAAACTTGTTGTTGTACGA ATACGTGTCGAATGGGAGCTTAGGTGAAATGTTACATGGTGCCAAAGGAGCACATTTGAAATGGGAGACGAGGTA CCGTATTGCTGTGGAAGCTGCAAAGGGATTGTGTTATTTGCACCATGATTGTTCGCCTTCGATTATTCATAGAGA TGTCAAGTCCAATAATATTCCGCTGGATTCCGATTACGAGGCTCATGTTGCTGATTTTGGCCTAGCCAAATTCTT GCAGGATGCTGGTGCATCAGAGTGCATGTCCTCTATTGCTGGCTCATATGGTTACATTGCTCCAGAGTATGCATA CACATTGAAAGTTGACCAAAAGAGTGATGTATACAGTTTTGGAGTTGTACTGTTGGAACTTATCACAGGTCACAA GCCAGTTGGTGAATTCGGGGACGGTGTAGATATAGTCAGATGGGTAAATAAAACAATGTCCGAATTATCTCAGCC GTCTGATGCAGCCTCAGTTTTAGCAGTCGTTGACTCGAGGCTACATAGTTACCCTCTTGCAAGTGTTGTAAATTT GTTCAAGATTGCTATAATGTGTGTTGAAGAAGAGAGTTGTGCTAGGCCTACTATGAGGGAAGTTGTTCACATGCT TACAAATCTTCCTCAGTCTACTACTACTACTACTACTACTCTCCTTGCCCTTTGAAATTGCACCGATATCAAGTG TCTGGTTGAAAACTCGTGGAGTTTGAGGCCGGGAACACGAGTCTCATGAGTCTATTTGGGTACGGGGAACAA >StCLV2 (From clv2-7; (SEQ ID NO: 11) ATGGCAGAATCAGTTCTTGAACCTTGTACAACCTCTTATTCCTTCAAAGTTTCAATCTTTATCCTATTCTTCTTG ATTTTCCCTTTCTTGAACCCATTTTCATCTGCATTTCCTCTTTCTTTTGATACTAATGCAACTGAGGCTGTCAAT CTTGAAACAGAAGAGGACATGGGTTTGCTTTTGTTCTTCAAGTTACAGTTTCGAGAAACCCCTTTACCAAGCTGG GATGTCAATGTTCCTCTATCAAACTGGACTGGTGTTACCCGGTCTAACCAGACCGGACGGGTCACTGGACTTAAC CTCACAAGGTTTAACTTGTCAGGACAGGTTCATCCTTGTTTGTGTAATCTTACTTTTCTTGAAACCCTTGTGTTG TCTCATAATAGCTTTAACAATTCAATACCTTCTTGTTTATGGAAGTTGTGGAGCCTTAAGACCTTAGATCTTAGC TATAATATGCTTACTCTTCTTATTCCTAGTACATTTGCAACAACTATGAGTAAGTTAATTGAGCTTGACCTTAGT CATAACATGTTGAGTGATGAAATCCCAATGTGGATAGGGAATGTCTCAATGTCACTTGAAAAACTTAACTTAGGG TTTAATAGTTTTCATGGGGATATACCTAAGAGCTTGTTGAATTTGATGTCTTTGAAGTATCTTGACTTGTCTCAC AATAGTTTGATGGGAAATGTGGGTGATTTTAACCAAGAATTGGTCTCACTTAATCTTGAGTCTAATTTATTATCG GGTACTTTGCCTTGTTTATATTCGTCAAGGGAATCACTTACACTTCTTAATTTAGCAAACAATTCGATTCTTGGA GGTATACCAACGTGTATCTCGAGTCTTGGGGGTTTGACACAGCTCAACTTGTCACGTAATGAATTACGATATGGT ATCTCGCCTAGACTGGTTTTTTCAGAGAGGTTATGTTTGTTGGACTTGAGTTATAATGAGCTATCAGGGAAGATT CCAAGTAGGATTGTTGAGGCATCGGACAAGTCTGGACTTCTACTTCTTGACCTGTCTCACAATCAGTTCTCTGGT AATATTCCTGTAACGATAACAGAATTGAAGAGCTTGCAAGCATTGTTTCTGTCTTATAATCTTCTTGTGGGCGAA ATACCAGAAAGGATTGGTAATTTGACCTATCTACAGGTGATTGATCTCTCACATAACTTCCTCACCGGCTCGATT CCTTTGAACATCGTAGGATGTTTCCAACTACTGGTGCTGATACTAAACAGTAATAATCTTTCTGGGGAAATTCAG CCAGTGCTTGATGCGTTGGATAGTCTTAAGATATTTGATATAGGAAACAACAAGATTTCTGGTGAGATCCCACTG ACATTGGCAGGCTGCAAGTCGTTGGAAGTTGTTGACTTGAGCTCTAACAATCTCTCAGGGTCTCTAAATGGTGCA ATAACCAAATGGTCGAACCTCAAATTCCTCTCCCTTGCTCGGAACAAGTTCAGTGGATCTCTGCCAAGTTGGTTG TTTACATTTCAGGCTATTCATACTCTGGATTTTTCTGGAAACAAGTTCTCGGGATATATACCAGATGGTAACTTT AACACTAGTCCAAATTTCTACAACGGCGACATTAGGAAAACCATTCCTGCAGTACCATCAATTTCAGCTCGAAGC CTGGATATCAAACTTTCACTCATTGCTGATGAAACTAGTTTGAGCTTCAACTATAACCTGACAACCACAATTGGA ATTGATCTGTCTGACAATTTGCTTCATGGTGAAATTCCAGAGGGTCTGTTCGGATTACATGGTTTGGAGTACCTT AATTTGTCATACAATTTTCTTAATGGTCCAGTTCCAGGGAGTTTAGGGAAGTTGCAGAAGCTAAAAGCACTTGAT TTATCACATAATTCTTTATCTGGCCACATCCCTGAAAACATTACTGTCCTCAGAAATTTGACAGTTTTAAATCTG TCTTATAATTGTTTCTCTGGTGTTATTCCGACAAAGCGAGGTTATTGGAAATTTCCTGGAGCATTTGCTGGGAAT CCAGACTTATGTATGGAATCATCTGGTAATGTCTGTCAAAGAACTTTGCCTGTAGAGCCAGGGAAGAAATTTGAA GAGGAAATGGAAGAGGGACCATTATCAGTTTGGATTTTCTGTATAAGTGCTTTAGTTAGCTTCTATGTTGGCATT GTTGTTTTATTTTGTTCATCTCGAACAAGAAGCTGTATTCTGCAAACAAAAAGTTTAGCAGGTTGA >StACR4 (From ACR4-3;) SEQ ID NO: 12 ATGTCTTCAATTGCTATTTCATATGGTGAATATGGTTCTGTTTTTTGTGGGTTGAAGTCAGATGGATCTCATTTG GTCAGCTGCTATGGCTCTACTTCTTCTATAATATATTCAACTCCAGCTCATTTCCCTTTTATTGGTCTTACTGCT GGAAATGGCTTTGTATGTGGACTTTTGATGGATTCTTACCAGCCTTATTGTTGGGGGAAAAGTAATTTTGTACAA ATGGGAGTGCCTCAGCCTATGATCAAAGGGTCTCAATACTTGGAAATATCTGCAGGTGAAAATCATTTGTGTGGA CTAAGGCAACCTTTAATGGGGAAGCATAGGAACACTTCACTTGTTGATTGCTGGGGTTATAACATGACCACAAAT AATGAGTTTGAAGGTCAGATCCACTCTATTTCAGCTGGTTCTGAGTTTAATTGTGCTTTGTTTTCTGTCAATAAA AGTGTTTTATGTTGGGGGGATGAAACTAGTAGCCAGGTTATTACCCTAGCACCAAAAGATTTGAGATTTATTAAG ATTGCAGCTGGGGGATATCATGTTTGTGGGATCCTAGAAGGGGTGAATTCTCAAGTGTATTGCTGGGGAAGGAGC ATGAACCTTGAAGAAGAATTCTCTGTTGCTCAACTCAATGTTGAATTGGCAGCCCCTAGTGATCCAATTATATCT GTTGTTGGTGGTAAGTTTCATGCTTGTGGGATTAGGAGCTATGACCGTCATGTCGTTTGCTGGGGTTACAGAGTT GAGAAAAGCACACCACCTCCTAGTGGAGTTAGGCTTTATGAGATAGCAGCTGGTGACTACTTCACTTGTGGTATC CTTGCGGAAATTTCACTTTTGCCTGTTTGTTGGGGGTTTGGTTTTCCCTCATCGCTACCACTCGCTGTTTCTCCT GGAGTCTGCAAGCCTAGACCCTGTGCATCTGGCTTCTATGAGTTTAACAACGGAAGTGCAACTTGCAAGTCTCCT GATTCTCGCATTTGCCTTCCCTGCACCAATGGCTGCCCTGCTGAAATGTATCAACAGGTTCAATGCACTTCATCT ACGGACAGTCAGTGCACGTATAATTGTTCAAGTTGTACCTCTGTTGACTGCCTAAACAGCTGTTCTACTGCTATT TCTGGGAAGAAGAACGCTAAATTTTGGTCACTCCAGTTACCAGTAATTGTTGCTGAGGTTGCATTTGCAGTATTC TTGGTGAGTGTTGTATCTCTAACTTCGATCGTATATGTTCGCTACAAATTAAGGAACTGTAGATGTTCAGGGAAA GGTCCTAGTCCTAGGAAGAATGGTACTTTCCCAAAGGAAATTGCTAAAGATAGGGCTGATTTGGATGATCTTAAA ATAAGGAGAGCTCAGATGTTTACTTATGAAGATCTTGAGAGAGCAACTGAGGGATTCAAAGAAGAATCACAAGTT GGAAAGGGTAGCTTTTCGTGTGTTTTCAAGGGCGTTTTGAAGGACGGTACTGTGGTTGCTGTCAAGAGGGCTATA ATGTCATCTGACATGAAGAAGAATTCAAAGGAGTTCCACAATGAGCTAGACTTGCTGTCCAGGTTGAATCATGCT CATTTGCTCAATTTGCTAGGTTATTGTGAAGAAGGTGGAGAGAGACTTCTAGTTTATGAGTACATGGCTAATGAC TCGTTGCATGAACATCTACATGGGAAAAAGAAGGAGCAATTGGATTGGATAAGAAGGGTAACCATTGCAGTCCAA GCTGCTCGGGGAATCGAATATTTGCATGGTTATGCATGTCCACCTGTGATTCACAGAGACATCAAGTCCTCAAAC ATCCTTATAGATGAAGAACACAATGCTCGAGTAGCTGATTTTGGGCTTTCCTTGCTTGGACCTGCTAATAGCAGT TCCCCATTAGCTGAGTTACCAGCAGGGACACTTGGGTACCTTGATCCCGAGTACTACAGACTACATTATCTTACA ACCAAATCTGATGTCTATAGCTTTGGTGTTTTGCTTTTGGAAATTCTCAGTGGTCGGAAAGCTATTGACATGCAA TACGATGAAGGGAACATAGTGGAATGGGCAGTCCCATTAATCAAAGCTGGTGAAATAGAGGCAATACTGGATCCA GTTTTGAAATCACCTTCTGATGCTGAAGCTCTTAGAAGAATCGCTAATATAGCCAGCAAATGCGTGAGGATGAGA GGGAAAGAGAGGCCGTCAATGGATAAAGTAACAACAGCTTTGGAGAGAGCACTTGCTCAATTGATGGGTAGTCCA AGCAATGACCAGCCTATCTTGCCAACAGAGGTTGTTCTAGGAAGCAGCAGAATGCACAAGAAGTCCTCATCAAAT CGATCAACATCAGAAACAACAGATGTTGCAGAAACTGAGGATCAGTGGTATGTCGAATTCAGAGCTCCTTCGTGG ATTACATTCCCAAGTGTAGCATCATCTCAGAGAAGAAAGTCTTCAGTATCGGACGCAGATGTTGAAGCAAAGAAT TTAGAAAGTAGGAACTGTGGAAATGGAACTGATGGATTGAGAAGTTTGGAAGAAGAAATTGGACCAGCTTCTCCT CATGAACATTTGTTCTTGAAACACAACTTCTAA StERL2 (SEQ ID NO: 13) ATGGAGGTGAGCGTGAAGATGAAATTCCCCTCACAAGCACTACTGTTGGCTCTATTGCTT GTTTTACCGATCGTTTTAGCTCTCACCGAAGAAGGCAAAGCATTAATGTCGATCAAGGCA TCGTTTAGCAACGTGGCAAACGTGTTGCTAGATTGGGATGATGTCCACGACGAGGATTTT TGCTCATGGCGAGGCGTGTTGTGTGGAAATTTCTCCATTTCCGTCGTTGCCCTTGATTTG TCTGATAACTTGCTCTATGGAGATATACCTTTCTCAATTTCTAAGCTCAAGCAGCTAGAG TTATTGAACCTGAAAAACAACCAGTTGTCTGGCCCAATCCCATCCACATTAACTCAAATC CCTAATCTAAAGACGCTTGGCTTAAGAGGCAACATGTTGACAGGAACATTGTCCCCTGAT ATGTGCCAGTTGACTGGTTTGTGTGATGTGCGGGGCAATAACCTCAGTGGAATAGTTCCA GATAATATTGGGAATTGTACAAGTTTTGAGATACTGGATATCTCATACAATCAGATAACT GGAGAAATTCCCTACAATATTGGATTTTTACAAGTGGCTACCTTGTCTTTGCAAGGAAAT AGGCTAACTGGGAAGATCCCAGAAGTGATTGGTCTAATGCAAGCTCTTGCTGTTCTGGAC TTGAGTGAAAATGAGTTGGTGGGACCAATTCCTCCAATCTTTGGCAATTTATCCTACACT GGGAAACTGTACCTGCACGGCAACAAACTTACAGGGCCAATACCACCGGAGCTAGGAAAT ATGTCTAAACTTAGTTACTTGCAATTAAATGACAATCAGCTAATGGGGCGAATTCCCTCC GAACTTGGCAAACTGGACCAGTTATTTGAATTGAATCTTGCAAATAACAAGTTGGAGGGA CCAATTCCTGAAAATATCAGCTCCTGCTCGGCATTGAATCAACTTAATGTTCATGGCAAC AACTTAAACGGGTCCATTCCTTCAGGGTTTAAGAATCTTGAGAGCCTGACATATCTGGAT CTCTCTGGCAATGAATTTTCTGGGTCTATCCCTGGTTCTATTGGAGATTTGGAGCATCTC CTCACACTGAATCTGAGCAGCAATCATCTTGATGGACAAATTCCTGTAGAATTTGGCAAT CTGAAAAGTATACAGACCATTGATATGTCATGCAACAAGATTTCTGGTGCCATCCCAAAA GAGCTGGGACAGCTGCAGACCATGATAACTCTGAATATATCCTACAACAATTTTAGTGGT GTTGTTCCTCTTTCACGGAATTTCTCGCGGTTTGCACCTGACAGCTTTTTGGGGAACCCA TTTCTTTGTGGCAACTGGAAAGGCTCAATATGTGACCCCTATGCACCAAGGTCTAACGCC TTGTTCTCTAGAACAGCTGTTGTTTGCACAGCATTGGGTTTCATAGCACTCTTATCCATG GTTATAGTGGCAGTGTACAAGTCCAACCAACCACACCAGTTTCTGAAGGGGCCTAAGACC AATCAAGGTTCCCCCAAACTTGTGGTTCTTCACATGGATATGGCCATCCATACATATGAT GACATTATGAGGATTACTGAGAACTTCAATGAGAAATTCATCATAGGATATGGTGCTTCC AGCACTGTATATAAATGTGTTTTGAAAGATTCCCGACCGATTGCCGTTAAGCGACTTTAC ACTACACATCCGCACAGCTTGCGAGAGTTTGAGACTGAACTGGAGACCATTGGAAGCATC AGGCATAGAAACCTTGTTAGCTTGCATGGTTACTCCCTTTCCCCTCATGGGAATCTCCTT TGTTACGACTACTTGGAGAATGGTTCACTCTGGGATCTACTTCATGGGCCTTCCAAAAAG GTGAAGCTTGACTGGGAAACACGTCTGAGGATTGCTGTTGGTGCTGCTCAGGGTCTTGCT TATCTTCACCACGATTGCAACCCAAGAATCATCCACAGAGATGTGAAATCTTCAAACATT CTTGTTGATGAAAATTTTGAGGCTCATCTTTCTGATTTTGGGGTTGCAAAATGCATCCCT TCTGCAAAAACTCATGCATCAACTTTGGTGTTGGGCACCATAGGTTACATTGACCCTGAG TATGCCAGGACTTCCAGGTTAACTGAAAAATCAGACGTCTACAGCTTTGGCATTGTTCTC CTAGAGCTTTTGACAGGAAAGAAACCGGTTGATAATGACTTGAACCTGCATCAGCTGATA ATGTCAAAGGCGGATGATAACACCGTGATGGATGCTGTTGATCCTGAGGTATCTGTTACA TGTATGGACTTAACACATGTGAGGAAAACTTTTCAGCTTGCGTTGCTGTGCACAAAAAGA TTTCCATGTGAGAGGCCAACGATGCATGAGGTTGCTAGGGTACTTGTTTCCTTGCTTCCT CCCCCGCCAACCAAACCTTGTTTAGACCCACCTCCCAAATCCATTGATTATACAAAGTTT GTGATTGGGAAAGGACTACCGCAAGTTCAGCAGGGTGATGATTCCTCCGAAGCACAGTGG CTTTTTCTTAGATATTTAGCTGCTGCACTGGTTCAATGGAACGAGTTTGAAGATGGTGAA GAATTGCATCTATGTTGA

Example 5 Inhibition of Plant Responses to Nematode CLE Peptides and Inhibition of Nematode Infections by Inhibition of a Plant CLV2, CRN1, and/or BAM1 Gene

Mutant Arabidopsis plants i) homozygous for the recessive bam1-3 mutation; ii) homozygous for the recessive clv2-6 mutation; iii) homozygous for the recessive crn-1 mutation; iv) homozygous for both the recessive clv2-6 and bam1-3 mutations; and v) homozygous for both the recessive crn-1 and bam1-3 mutations were exposed to the cyst nematode Heterodera schachtii and assayed for a response as described in Example 1. More specifically, sterilized receptor mutants were plated in 12-well Falcon tissue culture plates (BD Biosciences) containing modified Knop's medium with 0.8% Daishin agar in a randomized block design. Plants were grown at 24° C. with a 12 hour photoperiod. Fourteen days after germination, seedlings were inoculated with 200 surface-sterilized BCN (Beet Cyst Nematodes; i.e. Heterodera schachtii) J2. J4 females were counted at 14 days post-inoculation (dpi) and adult females were counted at 30 dpi. The average values were calculated and significant differences were determined by using Student's t test (P<0.05). To measure syncytia size, receptor mutants were germinated on modified Knop's medium in vertical square plates and inoculated at 10 days after germination with 10 surface-sterilized BCN J2. At 14 dpi (days post infection) and 30 dpi, syncytia that were transparent and only fed upon by only one nematode were visualized with a Nikon Eclipse TS 100 inverted microscope. Area of syncytia was measured using Adobe Photoshop CS5 and significant differences were determined by using Student's t test (P<0.05). In these experiments, the bam1-3 mutant exhibited a 25% reduction in nematode infection that was similar to reductions obtained with clv2-6 mutants. The clv2-6, bam1-3 double mutant and the crn-1, bam1-3 double mutant showed a 35% and 50% reduction in nematode infection respectively (FIG. 15).

Example 6 Use of a pCLV1 Promoter to Drive Expression of Heterologous Genes in Nematode Infected Roots

The promoter for the Arabidopsis CLV1 gene was operably linked to a beta-glucuronidase gene (GUS) and introduced into transgenic Arabidopsis plants. The transgenic plants were then infected with BCN and expression of the GUS observed. It was determined that the pCLV1 promoter can provide for expression in the root vasculature uninfected plants as well as upregulation of expression at sites of BCN infection in plant roots (FIG. 16). The sequence of the pCLV1 promoter is provided in Table 4.

TABLE 4 Arabidopsis thaliana Clv1 gaagacccaaggcccaacgacctactggtcca promoter and 5′UTR ggttgactatgaacaaaagaactagatttttttttcc (SEQ ID NO: 14) cctacattttaaagaaaatacttgatgaagatgtg gtgccttttcataagatctaaaaagtttcaaatcttta cgatggaacaaaaagtgaaaggtgaagtaagg gtcatttgggattgagaagtttcttcgtccaaaatca ttgcatgagttgaatagatttgggattaaagctgcc aatacaagaggattcggtaatgactgaagcaaa agcccagcagggccattaggcaaacaccagttt ccaagacggatttgtgtaagaccacttatgacac aagtttgtcttcactatcatcatcttcttcttctacttcta ctactacttttgtagccttgtcgtttttatcattaacatg attgacaagactatgacctatatatcttattattatca ttgctctctctatttgtttatattgattattactttttgagat ttttcaatggttttatctctaactaaacattataattagt gaaacaagcttagtagaagtaaaagtattattcta tgctaaagtacattgattagtagagtgtgtaattgtg tatacagataatctataaacaattggtgcatctgtat ataaaactttatgatttatttattgtatttaataagtata tgaattgggtacctaactttctaaacagttccaaatt tattcttaaatcaaattgcatatgatttttaaatattttg agacgattttattataacgcaaacaacagagtaa aagaagcatatgttgcaaattgtactatggcaagt tcaaatcgaaacatttttgtgaaaatcaaacatgtg aaccaagcttctacagtttaattccctttcgtataatt taatttcaacaaatttattgatatccatctagaaattg gtccaaagttctttcacccttgagtcatttagtgata aagatgacatgatttttggtgataaattttccatcgtt gctatatgtcgttatattattctcctatatgtatattata ctatttacatcagaaaataatccaaagtttagagat tcttttttacaataataaaatttcccacttactaaaaa gagctccttttctgctgaagagaacctaaaccttta ttcccaaagttcattgagttagagcattttcagcga atcacataagagatgctctcttcttcatcactaattg acatctcattgttttaaaggttgcacttgtacctgttg atctgattctcaatccacttaagttaaaccaaatag acacgagaaaaaagcacatttatttgttgctaagt atgcatatttttcagcgtttacttcttaatctaatgtata tcataagataatatctaaaagagaatgcacaaa agattattaatatgagaaattcgctgccatttagga aggacctttataccaatataccgcaataataatag aacattggtccccaagtgtatgtcaaccccaagtg tatagatttctttaaagattaaaatccctttttgttgcta aagcacctgatatatttttctatcaaactaaaaaaa ttgttagcgggatgaagatatattcgccaagaacc atagtgcttgtataacggcagaccattaattcaca actattattattttattgttagattgttgatagaatcgat tttgattgtggcagaatcgatcttgtaaaaactgcttt aaggtgcttacttataattaagaaagattcacttat gtaagttaagcatattaatcatatcattcggcctaat tcattaggaatattttgctattcgttttgccatcattaa caacaaaattgacacgttttcagccaaaagtatta acaactaaacctaaaacttcaaacattaaatagtt tttagtatctttagtttcaaactagtgatttgtcctaata tcaacactacgaacgaatttatatacattgaactttt ttctgaatcaccgattacaaaacgaatataatttgg tatcggcagttgctattaatttgatcggtttggactttg gactaatcacgatcaaatcttaaatggaccgaag tgaataaatccctaatgttttcaagagagtcacac gaacgaaacaaaggtaaaatatgaacatagag cgtggggaccttgaagcagaaggtctgtatggtg acagaccggtgagtggagtgtatgaatgaacga gaagtgagaagacaaaatacaagaaagagcg ttgacttggaagttaaagccaaaaaaaccacaa ggggcaaatttgtctctttaggaaaaggacacag acagactttctatacgggccaattagaaaaatag gccctacttctaattaaagcccatttacttctctcctt gtcttcttattcctcttttctccccatcacgtgacgac gatgctataaacgccgtcggattatataactggtg ccgttgacaagacggcgacagaagaaagaaa gaagaaaccacaggctctagggaacgtaacgtt atgtcctgtctatagcatttataacggtcagatcaa cgccgtttagataaagatctgtcaatgttaaagaa gagatgcatctctacaccgttaaatttaaaacgcc gtgaacctcttatctattgatttttgtttgatgaagcca aaacaaatcgtgtcagaagacttatcagagaag aagaaaacgacgacgttcccgtttctccatgtcta ataagtgtagtagtggcggctactaaaaactctaa agtttgactccagtaaaactgcctttctagtgtaatt ccagtgattttagagtttgaatagtgtgtgaccaaa tttgaaagtacaatctcagcaatattattgatcactc gttataaaagaatcgaatgtaaaaatagccaatg agagactgagacgtatgtgtttgaccataagtcgt atagtttgtatctatctacctgcaagatcagcagat ggttctctgatcaattgtaccttaattatcttttattttcg taaaatttctctattcacaaatgataaatctacttaa gacagtaaccataacaagatttacaagataatttg aaaaatgaacacataaaagtattttggcgcattat ttttaataataacaatatttatgtaaagtcacataaa agtatatattcgctcacaaagtcttacggtatttaga acagtagtaccacatcgattctcttcatcttcttcttc ataatatgccattgttcatgtctctgtgtcctatcgca taacactcacgctatcttattattttctctcgctctttct cactgagaggacactaaaaaa

Example 7 Nematode-Inducible Expression of the Potato CRN and CLV2 Promoters

The promoter for the Solanum tuberosum CRN and CLV2 genes was operably linked to a beta-glucuronidase gene (GUS) and introduced into transgenic Arabidopsis plants. The transgenic plants were then infected with BCN and expression of the GUS observed. It was determined that the StCRN and StCLV2 promoters can provide for expression in the root vasculature uninfected plants as well as upregulation of expression at sites of BCN infection in plant roots (FIGS. 17 and 18, respectively). The sequence of these and other nematode inducible potato promoters that can be used in the methods of this invention are provided in Table 5.

TABLE 5 Promoter Sequence Solanum tuberosum CTCCGAGCGTGAAATGAAATTAATTCCTTTAGGAGAACATAAATGTCTGCA >StACR4 (clone 3) GAACTATTCTTGAAACCTGGCGCAGAGGATAAAATAAATATTCAATCTATC promoter sequence; (SEQ TAATAATAGTTGTTCACTCGCGCATTTCTTATGAAACTATAAATAGAATGA ID NO: 15) TAATTTACTATATCACCTTTTGAATATACTCTCTCTGTCCCTAATTACTTG TCCATTTTGATAAATCAAGAAAGAACAATTTTTTTTTTATCTATTATACCC TCAATAAATTACTTTGAAACTGTAGAGCTTCTTGAAAATCTCAAGTTTTTA ATTTATCCACTTCATAATTAATAGGGGTAAAATGGTAAACTACTATGCCAA TAATTGTTTTCTTAATATGTGTGTCAATTCAAAAGTGGACAAATAATTAGG GACATAGAAAGTAAGAGATACAATATCTTGAAAAATGTAATAGGGAAATAA CTATAATTAATGATGAGTAAATTATGAACTAAGTGTAAAATTATTTATTGA TGTCATAAAGTAGACAAATACTCTCTCTGTCCAATAATAGTTGTCCACTAT TGACCTGACACACCCCTTAAAAAATAATAAATATTGTAATACTACTTTATT ATCCTTTGACTTTATTAAATTTAATGTTTTGAAAAATGTTTTAGATGATAA ATAATACCCTCTATCCCTAATTACTTGTCCACATTTTCTTTTTTAATTGTC CCTAATTACTTGTTCATTTTAATAAATTAAGAAAGGACAATTTTTTTTTAC CTATTATACCCTCAATTAATTATTTAAAAAAAAGTAGAACTTCTTGAAACT ATTAAGTTTTTTAATTCATCCACTTCATAATTAATATGGGCAAAATGGTAA ACTCATTATGTCAATTATTGATTTCTTAATAGGTGTGTCAATTCAAAAGTG AACAAATAATTAGGAACAAAGAGAGTATTTAATAGCAAGAGTAAAACAAAC ACAAAAGGTAAATTATATCTCTTAATTTTCTAGATTGGACAAATATTGATG GACAACTATTTTTAGTATAGTGGATAACTATTGTTAGACAAATAAAGTATT GTTGAATATCCCAAAATAATATAATGGACAACTATAATTAGGCGGAGGGAG AATTATTGTTGGACGGAGGAAGTAGAAACAAAATTTTTAAAGCTAGCAATT TTAGGATGATTAGGGGGGATTATGATAATGATTGTACTAAGTAGGTACAAT TATAATGGAAATTTTAGTTAATTATGGTGTACTCTGTAAGAAGAGAGAAAA TTTGAATAAAATTAAGTAGTAGTTATTTGTAGAAAGTAAGGGAGGACATGT GTGCAGGTATCCAGGCATTGAAATATCAATTTTGCAATAAATTTTTCATTA AATGCTTTCACCTACACTGCTCTTATTTTGAGAAGATGTAGTTTTGAAGCA TTTAATGCTCACTTTTCTCTCTCTATTTCTCCTATGCTGTCTTTCACCACT TCATTCTTGAGGGCACCGATAACTTTGACACAAAAGGGGCTAAAAAAAATG TCATTATGTTCTCTTTTTTGTGTTTCTTGAACTGAAATATGCAGCTTCTTG GCTACAAATTTTGTTTAATTGTATTGATAACGAGGGTATTATAATTATTTG GGAGGAAGGAAAGTTGTGAATTTTGATCTCATCTACCCACCCATGGTATGT TTGAACTATTTTTTTTCTCGTGTGTTTCATAAATTAAGTCAGCTACTATGG AGAAGGAGGAGTGGTATTTTGGTTCTATCAAAAAGGATAAAGGTGAAAGAA AGCACTGACTTTCTGTTTGTGTACTTTGTTTAATTTTTAATTTGTGTAATG GACGTGTTTAATAAGTGGTGTGTGGTGATGTGGAAATGTAGATACTTTGTA AGAGCTTTTATGCTTCTTGTAGTATTTTCAAAAGTATCAGGGTTTGGATCA ATG Solanum tuberosum CGATGAATAATGCTCCCTATCAATATTTTTTTTATACTGAGAATCCAAAAA >StCLV1 (clone CAATTATAATGACCATGCTGAATTTCAGAAAATGGTGGGACAGTTATGAAA 5)promoter sequence; AAGATGTTGAGATTGGTTGGATCGGAAGAAGAGAACAAAAAGAGTGGACTT (SEQ ID NO: 16) TTTACGCAATGCCAAAATTACAGAGTGTCTCTTACCCAAGGACACATACAG ACTTTGCCAATGGGCCCCAGAACCACCCATTAACCCCCCCCCCCACAAAAT ATGGGCCTTCCTACCATACCAAAGAAAAAAAAGAAAAAAAATTACGAAATA ATTATAAGATCGATAATGTTATATGATAATGAATATTGGAGCGTAAAACTC TAATAATTCATAATGGGAGGTTTGAAAGCAAAAATGAGTCTAATAATATAG AAATACACACAAGATAGATGCGCAGAGATTCGACTGTTAAAATAATCATGT GGTGAAATTATATACTAGATAAAATTAAAAATGACTAAATACATCAGTATA TCAATCGTTGCATTGATCAATAGATACACTAATTATCATAGCTATAAAATT ATAATAAGTAAAATACTGTAACAAAATAGCTCCTTCAAATCATATAAAAAT CTACACTAACACAATAAATAGATTTAAAAAAAAATATAGCCTAAACAACAA ATACCACACTCTAAATATGAGTTACGACCATTTTTTTTTTTATGGTTTTGT AAGGAATTAGGGTGCAAATCATTAAAAACGAAGATAAGATGTAAGTAACCA AAACGTGCATGCATTAGGATGCAAATCACAAACTACTCAAATTTACTATTA GAAGTGCTCATTTTAATAAATTTAGAGGACCAAAGTGACAAAGAGTTATAC TTAATAAACTAGTTTGAAATAACCCAAAGATAAAGATATTATTTTTGTTAT TTTCTAGTATAAATCTTAGCTGACAGACTCAGAAGCGTCAATCATCAAAAG AAGTTCACAAAAAGCCGTCAGTAATTTACTCTGTTTCTCATCTAACCATTG CTTCTAAAGTCTGCTGCTACAATCATTTTACTTGCATCTATACATATCACC ATGACTTTTTTTACCAATAAATATGAGATTTAACCGTAAGTTATTGAGTTC GATTGAATCTTCACGTAACATAGTAATTAAATATGAAATTATACCATGATT TAAAGCTAATTAAATATGAGATGGAATTATCGAAAATTATGATGAAGTAGT CAATACTTTTTCATCTTAACAAGAGATTTTGAATTTAAATTTGAATTTTGG ATGAAGTTACTTTTGATAAATAATGTTTTAACCTTAAACTAGAAAAGGTTG ATTCGTTCATTAAAATATATTCTTTCAATCTCAATTTATGTAACACTGTTT GACCTAATATAGATTTTAAGTAAGATAGAAAGGAAATTTTTATAAATTTAT GATCTAACTCCTCATTTTGGTGATTATAAATCATTTGATTAGTAAAGAGTT TTTGAAGTTACTCTTTTAAAATATTATAAAAATGATAATTTTTAATAAAAT AAAAGAAAATTATATATTTTGTTGGGTCTTTTAGTATCCAATATCCATATT TAAACTCGATTAGTTCTAAATTAGCGCTGAAAAGTGTTACAGTAGTTGTAC AAAATTCTCTAATAAAAGTGATTCCGTATCGTATTTAAATTTGAAAGCTTT GATTATGAATGATTAAGAATGGAGGAACAAAATTTGTTACCTTATTATTAT TTGGTAGAGATGAAGTATTTACCACTCCCTGTGGTATCTTCACTTTGTTTC CTNACACACATATATTCAAAGCCAAAAAGTTAATTTTGATTCTCCTTCCAC TTTGGCCAAATGCAACAGTACTAAATACTCAACACTTCAAATACCCTTGAA CCTATCCCAAAATTTGTACAAACCAGACTAAACTAACAGTGTAATA Solanum tuberosum CTCACTCGCCTCTCTCATCCCTCTCACCTTTTTCCTCCCTTTCCCATTCTC >StCLV2 (clone 7) ACTCGCCAGATATACAAATACATATGTATACTAGTTACATACAGAATGATA promoter sequence; (SEQ TACATACACAATTCAACAAATATACAAATTCAATTTACCTCTCTTCACTCT ID NO: 17) ATGTCCTCTCTCCTCCCTCTCCCAATCGCTCTCGTCTCTTTCCTCCCTTTA AAATATAGCTACAAATCGTAATTATCAAATTATAGCTATGAAGCCTAATTA AGTTATTTTTAATGGTTATTTGTGAAATTTCCTCTTTTTTAAAATAGTTTT TAGAAAATCAAACTTCAGTAACTTTTAAGTTAAAAAATAAAAAGTAAGAGT ACCTACTTTTAACTTTTTAAAAATCGTTTTTAAAATATTGAAATATTCTTG GCGACTAAAAACTACTTTTAACCTAAGCGAAACACCCTCTGAATCTTAGTA GAGTAAGTTCTCGAGTCATATCATGATTAATTTATTTTCACTCGTGTACTT TAGCTTTTCATTTTTCCTTAATTTTGTTTTACACTACTATAAAATAGTGGG ATGCATCTATATCTTATCGTTTTTTTATGTTACATTAATTCATCACTTTTA AAATAATAAAAGTATTTAGATATATAGTTTTTGCCAAAGTTTTATGATATT ATAAAACAAATTTGAAAATCAATCGAATCGAACTGACACTTAAATAATCGT GATAATATTTAAATATTATAAAATAGAATAACTATAAAATTAATATTATGT AAATTTAATAAAATAATCGATTGAAGCGTACCATTGAAAACTCGAAAGTGA AAGGAAGAAGAGCATAATTGTTGATATGGGTTCACACGCTCACTTACATAC ATATAATAAAGGCTCTCTTTAAAGAGAATTTGAAAAAGAAAAGAAAAGTGA AGTTGTCTACTTTACTTTAGTTTTACACTTCTCCAGGCACGCCAAACACCT TTTGCCTCTCTTTTTTTTTTTTTTTTTCATTGGGACTGTTTTTTTTTTTTA GTTTGTTTTTCTTTTCTTTCATCAAAGAGGTATTTTTCGTTTCTATAATAT TGGGAGTAGCAAAAATGCTACTAGTATATGAAATGGCAATTAGTACTTTTA TTTATCATCAAATGATATATGGTGCAGTGTATACAATATTCAAATTCCGAA TATGAAAAAATTCATAATAGAAAGTACTTTTCCATAAGAGATCATACAATG AGAAAATATTCAAATTAATCAAACTCCAATACAGATACTATCAAAAATCAA ATGAATGAGAAAATAATAAAAAAATATTACCCCCATTTGATCACTCAACTT TTCTCTCCATTATTTAATACAAAAATATCAGGTGATTTTTCATATTTGTTC TAACTTTAGTATATAGAGTTATCTAGTACTCCCTTCAATTACTTTTGATAC TAATGCAACTAGGCTTGTCAATAAAATATTTCATTAGCTATTACTGATGAG AGATAACAAATATTTCATAAAATTAGTTGAAGTGCGCAAAAGACCAACCTC AAACACACAATCATAAAAAAAAAAGTGAGGAAATATAGAGTGTGTGCCTCT CAATAAAATAAGTACTAAAAAAGAAAACAAGAAACAAGAAAGAATGTTGGT TCTTTAGTGGTGACTCTCAATGAAGTACCTACTTTCAGCTTACTCTCTCTA TACTCACTACTACTGCTACTCAGTACTGATTCCTTTCACACATACTGTGCC TGTAAACCCTGTCCAGGGACCCCCATTTCCCCTTTCCCCTTTCCCCTTTCT CCTTCCTTCCTTAGCTATCTCTCACACAAACACTAATCTTTTTTCACCTCT ACCTTACCTCC >StCRN promoter TCCGTTTGAGGGATTTCTGTAATTATAAACTTTTAAGGGATAGATTGTAAT sequence; (SEQ ID NO: 18 TTTGCCTTCAAAATATGTGATTTCTGTAATTTGCCTTATTATATAAACAAT GTGTATTATCCGCATAATTACCACTTATAGTAATTGAATAGGTTTTACCAT CTATAACATAACTTTTTTAACAATTTGTCTCCCTCTCCCATTCACTCCCCC CCCCCCCCCCCCTCTCTCCTTCTTCTCTTTTCTTTCTGCCCGTCTCTCTCT ATAAATTTCAATTATCCTAATTTAAGACTTGATTTTGGATCGAGTATTATT TCCAAACAATTGAGAACATCTTTGAAATTTATATCTTAATGTTTAAGGATT GTTGATGAAGACTAATATTTATATAACTAATATATTTTTATTACAATAATA AATCTAACAATGTTTAAAAGAGAAAAAATAATAAATAATTATCAGTATATT ATACATATTTATGTTTCAATGCACATGGTGAATATAATTAAAATATTTTTA AAACAAATGTATTATAAGTATTATGTGTGAATCTCAAACATTTCAGTACTA TTTAAATTAGTTTACATTGTTAGAAATGTATTATATTTGTTGGAATAACAA CAATCAAATTCATAACAATGTATAATATTGAATTTGAATGGTATTACAAGT GTCTTCTATATTTAATACAATTGTAATACATAAACTAATTTGAATAACATT TGAATATTTTAAATACAAGTACAATGCATTTTTAACACCATTAATAAGATA GAAAATAACAATTGTAAAACATTATGAATCCAATATATTATACTTATTAGA GGCATATTTCAAAACACGTGGTGAATATATAACTAAAACATCTTTAATAAA AATGTATTATAAGAATTATATGTGAACTTCAAACATTCTACTACCATTTAA ATTAGTTTACATTGTTAAAACTATATAGAGGTGGCAAATAGTTGGATTTGG ATGGGTTTAAAATGATTTAAATAAAAATGGGTAATTATCCAATCCGTCCAT ATTCTATATGGGTAAATATGGCTTGGATAATTAATGGACAGATTGGATATG AGTTACCCATATTTCATCCACATTGATTGAAGAAATAAAAAATGAATTTAT ATTTTTTAAGTTTCTAAAGTAATTTTTTATTCTACTCACTCCCATCCCTAC CTCCAATTCACCCACCCCTAATTTTAGTTTGTTTTATTTTTTCTAACCCCC GCCCAGTTTTTATCCCCCTCCACCTCACCCGTCTTCATCCCCTCTACCCCC ACCTGCACCCACCCACCCCCACTTTTTTTTAAAAAAAAATTCTACGCCCCC CTCCCTCAAGAATTTCCAATTTTTTTTTTGTTCTTCCATTAAAAAAATGAG TTTCTTTTTAAAAATAAAAATTTACCCCCTCCGCTCCACTCCTATTTTTTT TTTTTTTTGGTTTTTTTAAAAAAAGTAACATTTTCAGAAAAGAAAGTTACC CCTGTTATAAACTAAAGTATAACAACTTACTCTTGCTTCTTTCTTTGTTAC AAGAGGGGTATATATAGTTGTATACACTTGTGCCCAAAGTGTGATACACGG ATAACTTCTTGCCATGTATACACTTTGGACACCAAGTATATCAAATGGCTA ATTAGTATACACCACATAGCATTTTGTGTGTGTATTAATCTTACAACACTT AACATATTAGTGTGGACATTCAATTTACAACAACCCTGAATTGTATTACAA CTATCATTTATATTTCATACAACTTTTCAAAGTTGTAGCTCTTCTTTTCCG ATGATTCACAATCACCGGATTATCAGTAGCTCAAATCAATCCCATTATTAC AAATCACCACACAGTCCACCCACAGTCACCAAACTCTCTTTTCCCCATATT TTTGGTCCAAACACCATGACCAAATTTGAATGCCGAAGAGAGTTTTTCAAT TGGATCTAAACATCGATTTTCATGAAGCTCATCGGAGCAACGAACACCATC AAAATTATGTTCAGATCTAACAACACCACTGATTTATGTTCTACTCTTCTA CTTAAACAATGAACATAGAAACTACAATCTTCTTTGGTTCTTATAATTACA AATTAAAAAACAATAACGTAAAAGAAAAAAGATGCATAGAGATTGGGCATC GCATGGTTTCATGGAGCTCCATGTTTTTTTGTTGGAATTTGATGATTTTCC AATTTGGTTATTATGTTGTTCATTGTTGTTGTTGAGTCTATTTTGTGGTGG TGCGGAGGTGAGAGCTTTAAATTGGAGTTGGGGTGATTGTTGTTTTGTTCG CCGGAGAAGCCATCTCCAGTGAGGTTGGTTGGAGAAGGAGAGAGATGAGGA GAGCAATGAGTAATTTCAACTATTAAAGGTAAATTGAATTAATATCTCATA CGATCACTCGACTTTAAATAGTTTATTTAGAAAGTCACTTAACTTTGAATT GTTCACTAAAAAAATCACTCAACCTTATTTTATAACTCAAAAGTCACTCAA CTATTGATGTTTTACTTAAAAAGTCACCTAAGTATTGATATATTGCTTAGA AAGTCACTCAATCAATTTAAATAATTTTCCATTAAATTTTATTGTAAACTA TTTTTTAAAGAAATAATAAGATTTCTATTTTAATTATCTTATTAATCCGCT CCAATTATTTAATTATAATTTTCTGAAAAAACGTATAGCAATTGACCCAAA AAAAAAACTTTTCCGTTCTAGTAGTTGTTTGATTGGAATTAAATATGTTTA AAAATTATCAAAAAAAAATAGGATGTTGGAATTGATAAGAAGTAATAAAAA AACGCACAGTAGCAATCTTTTACTATTTTAAAAAAAAAAATAGTTAAAAAC AAAAACTACATTTCAACGAAATTCAATAAAATAATTTAAATAATCATCTTA TTATTTTTTAAAAACTAGTTTAATTATTTTTTGTATTTTTACAAATGAAAA AATTATTTAAATTGATTGAGTGACTTTCTAAGTGAAACACTAATAACTGAG TGAATTTTGAGTTATAAAAAAATTGAGTGACTTTCTAAGTGAACAACTCAA AATTGAGTGATTTTTTAAGTGAACTATTCAAAATTGAATTACCATATGATA TATTAACTCAAGGTAAATTAGGCTATGGACTATAATAGAAAAAAACCCAAA AAGGATAATAATTAATCTAAAAGAATTCATATATATATAAAACTATTTTGT TTAATGATAAATTTTTGACCCATTGGGTCTTTAAAAAAAAAAGAGAATACT CCATCTTGTTATTTTGTAGGTATAAAAAAAAAGTAGTTCTATCTTTAATAG GTTCATTTCTTTAGTGGAGGAAAAAAGTGGATTTATTCACTAATCTTGTTT TGTGAGAGGCAAAGTTGTTACATATTTGGAATTTGAACTTTGTAATGATTC TATTCTTGTTCATTGTGAAGTTGTATATATTCCTCACTGTTCACTTTTATC TTATTTTATTATTTATATAATTTTAAAATTAGCTTTTTCAGCAAAAGATTT TTGTTCTTGAAGATTCGTTTCAGAAAGAGAAAAAAAGAAGAAAATGGTCAC ATTGTCGTCCTTGTGTAACATTCAGAGGAGTGAACCCTAAACTTGCCGACC CACAGAGAAAAACAACCCTAGTTTCC >StBAM1 (clone 6) GAAGGGCATAATTGCTACTTGGACAACACAGTATAATTAATAGGACAACGA promoter sequence; (SEQ AAACTTCGTTTCATAAACTCATTCTCTAGCTTAAGTATAATTAATATGCCC ID NO: 19 CTAAACTATTTGAAAAGGTCTAGATATACCCTCCGTTTAAAAGTTTGGCTC ACTCATGCCCTCGCCGTTCAACTTTTTGTCTAAATATGCCCTTATGGGCAT TAGTTGGCCTGCTGGACATATCTAGCTCATTTTCCATTTCTTTAAATGCCA CATGGAATTGTCATGTCATTTTGACTTTACCACATGACATTTATATGAAAA TGGAAAGGGATCAATTATGCCCGTAAAAAATTCGAACCCATAAACACCTAA TCCGACCCATAAATCAACCCCCCCTCCTTTTAGATAAACTACCCGACCCAT TTTCAATAATTTTGTTTAAATTTTTATTTTTTTCGGTAAATCCAGGAAATT AGTAATTGATTAATAAAAAATAGAAAAAATATGGGGAAAAAAAATTAACGC CAAAAATTCACAAATAAATATTGTAACCTTAAATTCAACAATTTTTTTATT TTTTTCCGGTAAATCCCGAAAATGAGTAATTGATTAAAAAAATATATGAAA AATATAAAATTAACGCCAAAAAATCACAAAAAAATCCATTTTTCATATAAA TGTCATGTGGTAAAGTCAAAATGACATGGCAATTCCATGTGACATTTAAAG AAATGAAAAATGAGTTGGATATGTCCAGCAGACCAACTAACTCCCATAAGG GCATATTTAGACCAAAAGTTGGACGACGAGGACATGAGTGAGCCAAACTTT AAACGGAGGATATATCTTAGACCTTTTCAAATAGTTTAGGAACATAATTGA CCCTTTACCCATTGCACAAAATATCATTCATTTTGAAAGTAAAAGCAAATC AAAATGACATGGAATTGGAATAGCACTTAAATGATACTCCCTCCTATCCAT TTTAGTTGTCACTGTTTACTAAAAATAACTTGTCAAAAATATTGTCATAGA AAACTATGAATACATACACATTATGTTATGATTGTTTAGATTGGCAGATCA GTCTTGTTTTTATATACATTTCTTTATGTTCAACTTGAGCTAAAGGTATCA GAAACGATATTTTTATTTTTTCAATGTAGGAGTAAATAAGAGTTTATTTTC TTTGTCTCATATTAATCATTTTTATTTTTACACGCATATTAACAAATCATA CGAAGATAATTTTACTAATTCACTTCTTAAAAACTTATTGAAATTTTAAAA ATAAATGTGAACACTTTAATTTTTTTTTTGCAAGGGTAACAATATAAGAAA ATTTTAATTAATGTTTTCTTGATTTAGTAAAATGGACAACTAATATAAGAC AATTATTTTTAGTAAAATGATCAACTAATATGAGACGGAGAAAGTAATATA TAAAATGTCATTCTTATTAATAATTTCTTAAGGAATGTGTAAAATAAAAAC ACGATAACTAATCTCTCCTCTATTGTGGCTTTCTTTGTGCCATACTCTACT GTCCAAAAAATATTACTACTCATCAAAAGAAGAAAGGGCTTTCCTTAAGAA TGACATCTTATCAACTACAAAACTAACCTAAAGATGAAAAAACTACAGACG TTAGTGGAGAATGTTTTAACACCCTAAATTAAAGGAGATAAAGATAAGTGA AGTGCTTTTTGTGACAAACGAATTGAATGGAATTTTATGCCTCCCTCCCAA ATACTCTTTTTAGCTAATGAAATCTCTTTAACTAGTAAGGACAACTATTCA ACACGAGAAAAAGCAAGACCAATAGTTGTTTTTTTCTACTCTACTTTTTAT CCGTGAAAAGATTGTGTAAATGTTAGCAACTTTATTATTTTTAAGGAACAA AAAAGTTGGTTCCCCACGTTACAAAAAGAGTTGGGGCCTCCTCTACTTATC TCACAATTCAAATTTATTCTTTATAATATAATAATCAATCCCCTCCTATTA TATATATTTATTTACTCAAAACAAAAGAATATACACCAAACGGATTACCCA CCCCCTCCTCACTTTTGCCTTTCTCACTCTCACTGAGTGAAACCGCAAACC AAACAGTTGGTGGGCATTAGATTAAGGAAGGAAAA >StBAM2 (clone 2) GCGTCAAAGTATGAAGCAGACAACACATGAACACACAATAATGATCGACTC promoter sequence; (SEQ CCACTTAAAAATATTATTATTTTTTTGTTAAAAGGGAACGAAAGCATTATT ID NO: 20 TTTATTCGTTCACTATTTTAAAATTAATTCTTATTTGTACTTATCACTTTT TAATATATTAAAAGAACTTTACTTTTAACATCAATTAAAATGATATTATGA TAAAACATTCCTAATCAAATGTTATTTCTTAAATATGTACAAAGTTTAAAG TGGATCAGTAAAAATGTTAATGAAGGTAGTAACTTTTATTTGTTGTTTATT TACTTTGTTGATGTGTTTGTAATTTATAATCTTAAAGAATAATTATTAGAA TAAAATGAAGAAAAAATAATTAATTCTATTTTAAATTAACAAATAATTTAT AGTAATTATTTTTAAAAATGACGATAAATAATTTAAAACGGAGGAAGTATT AACTGTATTAATAATTAATATTAATACCACTAATGATAATGAAAGTGTTAG TATCCTACATGAAAAGGACATGATTGACTACTTTCGTATAATTTGACAATG AATTGAATGGAATATTATTTTTTTCTACATATTTGTTTTTGTTGTTAATAA TGTCTTAAATTATTAAACAGTTATATAATGCTGAAAAGAGAAAAACAAAAA GTATTGAATTCTCCTCTTTCTTCTCTTCCACAAAAATTGNAAAAAAAAAAA GCAGCTCTTTTATTAATATATATATTTTTTCTTTATTTCAAGTATAAAGTT TATTTAATGAAAAAAAATACTTTTAAAATTTATTATTTTAAATATATCATA ATATTTATGTTACTATTAAAATATTTATTATGAAAATTAAATTAATTTCAA ATACATAAATGTATCATTCTTTTCAAATATCTTTTGACTATGGAAAGAAAT TGTAAAGTAAACGATGACTTTTTTATTTTTTTGGTACTTAATTGATTTTTG AGGAACAAAATAATTGTCCCAAAGTATAAAAATAAAAAAAGTTGGGACCTT TTCTCTAGTCTCCATATGAAAAAGACAATTCAGTACTCAGTAGATTCAAAA TATCCTTTAAAAGCTAGAGCTCTTTAATATACAATAAGAAACAAAATAATC ACAAGACGATAATTATTTCAATTTTAAATGTAAAATTTTAAAAAATATACA AGTTCTTTTTAAGGTTTCACTCATAGAGCTGTAAACATATTTTTAAGTCCA CATACAACTTCTAACTTCTAAATATTCANTTTCAATCTAACTTCAAACACT ACANTTTTTCAATAATAATCAATTTATGTCCGACGCTTATTTTGTTGATAA TTAGGATAGAATATTACTAGTAGATAGTTGAGTGTTATCACATTTTACGTG AATGTGAANNAGAGAGTGAGCTGACCTTCTTCTATCCTCTTGTTTTTTTAA GTAGTATTATTTAGTTATCACGTAGTTTCTTACCTTCCACGTATATTGTTA CCTATTGTTGTATTTATTTATTATCTTGCCATTTTGTTGTTTCTTTTCAAA TAATTTTACACGACGTGTGATAAGTGTTTTCCTTTTGAGTCAATGGCCTTT CAAAAACAATCGTTTTTACTTTATAATCGTGAGATTACATTCAATGTGTTA TCATTACATTGGATATGTTTAACATTACATAAGGATGAAGAACGAATCAAT CTATTCAAATATTAAATATTCATTAAAACAATACAATACGATATAACCATC CAAACCAAACAGAGTGTCAATTTTTTTTAAAATTATTTTAGTTTCTAATGT ATATATTCAAAAATTTCATATAAATACACATTTATAATATATCTGTTCGAT AAAGACACGTGAACATTTCTTCTTCTTCTCCACCATTTCTGCTCTGCTCAC TCTTTCCCCTCCACCATTGAAGAAAC >StER(clone 2) promoter CCGAACATCTTTAGGGCATCTCCAACCGAATCCTCTATTTTACTCTTCAAA sequence; (SEQ ID TATAGAGTTTTCTATTTTTTTCAGACAACCAACTCCAACTCAATTCTCTAT NO: 21) TTTACTCTCTAAAAATGAATTTTTTTTTCTCTCCTCGATATTATATTATTA TTTCTATTTTATTCTTATTTTCTTATTTCATGATATAAATCCTTTATTTAT TTTTTTCCAAATAATTACTTTATATAATTTTTAATGTGATATGAAATTATA TTTTATTCTAAAATTTTAAATAACATAAATTGCAGGAAAATATAATATAAT ACATAAATTAGGGGACAAATTCAAATAAAAGTGATATACAATTACATAAAT ACTCAATTTTTAAAATTATTACGTTGCTCCCATAAATGCTCTATTAATGCA TTACGGAGTTCAAAATGAACATTTTTGTCCTTAATTTTTTTATGTCTAGCT AAAAATTGTTCAAACCGAAGATTTTCACTAGCTAAAAATTATTCAAATCGG GGATTTTCATCTACCATCATTTCTATAGTTGGAGTTGGAGCCTCTACGGCA TCTTGAATTGGTGCATTGAGATCACATTCATTCTCAATTTTCATGTTGTGC AGTATAATACATGTAGTCATTATATCATGTAGCACCACTTCTTTTCTCCAA AAATGTGACGGTCCTGCAATAATTGCAAAACGTGATTGCAAAAAGTTCGAG GACAAGGCTCTACTTTGCAATCATCGGAGTCCAAGACGAAACTAAAATTTT AACGAAAAATTTAGAAACTATTAGTGATCCAAATGTTCGTGGTTACCTGCA ACGAGAACAACAACGAATACTTGAAAAAAGAAATCGACAATCACAACCGCA ATCACAACCATAATCGCAACAATTCTCAGAATCATATCCTAATTTTTTTCC GAATAGTGCTAAATTTGAAAACGACCTACCGAATTTCTAAATTATTGTTGT GATCAATTAATTATTATGTCATGTATTGTATTTTATCTTGTATTTAAATTA TTATGTTATGTATTATATTGTATTGTTATCTTGTATTTAAATTATTATGTT ATGTATTATATTGTATTGTTATCTTGTATTTAAATTACCATATCATGTATT GTATTTTTAAATTAATTTTTTTTGCGTATCCTTTATAATGAAAATTAATAA TAAAATAATTTTATTATTCACGAAAATTAGAAAAAAAGTTAAAATACTATT AATTTGAAATTAAAATAGTATATATTAAATAATTTTTTTAAAAAATATTAT ATTACATTTAAAAAAGAATTATGAATATTAGATATTTAATTAATGGAATTA TATGTAAAATAATATGTTAATTAGAAAGTAATAGAAATAATAATAAAATAA TGAAAAAGTAGAAATAAAGAGCGTGAATAGTAGAATTTGGAGAACTATTCA ACTCTCAAAATTTGAAAAATAGAGGGTGATTTGGAGGTGGGTTGGAGTGCC CATTCTCTATTTTACTCTCCAAATATAGAGAATGAAGAGTAAAATAGAGGT GGATTGGAGATGATCTTAGTGACATTTTTGATTCCGCCAATGCTCAGTTGG CGTAGTCGCTGTCAAACTTGAGAAAGGATTACCCCTTTAGGCTTGCACAGA CAGTGACTTATGATGAAATGAAGCCAGAGAAGGCACTCTGTTATCACACTT AAATGAAAATACATGTGTATGGACTAGCAATAAAAGGGGCACTAGTAATTT TAGTAATTGAAAAGCAAGTGTATAGAGAGAGATAATGAGAGAGAAAGAGTA AGTACACTACTACTGCTACTATCCCATATAGCTGTAATGTTGCAGGTCTGA TTTTTGCAGTTGCAGACCCCCTTCTTGGCACAAGCTCTTTTAACTTTTATC TTCTCAAATAATTCTCTCTCTCTCTCTCTCTCTTTTTTCTCTTTTTACATT GTGAGGAAAGCTGAACACCCCATTGTATGTATTAGTGTGAGGCCTATCTGC CACAAGGATGTGATGGAACACTATGCTTCCTCTGCTAAAACCCCCACAACC CCAAAACTCTTTTTCACTTCACATTTAATCACAATTCCTCAGTGAAATTAT TCTGTTGCTCTCTCTAATTTCAATTTCAATGTCGGTAAGTCCAAGACCTGG TTTTTCAATTCAAAGGAGCTGAGTTAGTGCAAACACTTGAGGTTTTGAGTT TTGACAGAGACTTGAGTCTCAGAGAAACTACC >StERL2 (clone 1) CCTGGGAGAAAATGAAAGCATGATCTCTTTCTTGTAAATTGTTTCTACCAT promoter sequence; (SEQ ATTTTTTTTGGCACGATAAATAAATTTATATAAAATTGTATGAGTGACACT ID NO: 22 AGATGACAAGTCACATAACATATATATTCAAATTGATTTGTATTATTTATA GAACGAAAGTCTACTGTTTAACCTTATATAAGTTACAATTTAGTTATGTAT ATAAGTTAAAATTAAATTAAAAGACATTTCGAAATAATATGATTATACCAT TTCGAAATTAATTAGAGAGAGAAATAAGATCTCGCAAAATTAAGTGTCTTC TTGAAATTAAGAACCATTTTTAGGAGATAATTATGTATTTTTTCATTTTTA ATTTGACACGTATGCATATCCACTATTTTGTTTTATTCCAAAGTGACCCCT ACTTCTTTTGGTAATTTCTTTGAGTATTTTAAACTCTAGTCCCCCTTTCTC AAGCAAAAAGGCTCACTCGCGCACGCGCGAAGAGACATTGTGACGCGCTGG ATGGAAAATCCAGAAGCGTAACTGTCAAAAAATAGAACAACTTTGGGAAAC GGGGTGACGGCCGCTGCCACCACTTTTTTCATTTCCAAACACTCATTAACT AACGTCGTTTCACCGCCGTTTACTGCTTAATGAGTATGAATTACACTCTAA TAGTCTATTTTTACTTATTTTTAATGTGTTTATCAAATTATATTTTTAAAT ATAATACTTTAAAAATATTATCATCAATAATAAGAGTAAATTAAAAAATAA ATGACAAATTGTTTCTTAAATTGTTAAATTAAACAATTAAAACTGAATATT TACAAAATACCTCTTAACTTGCTAAATTAAACAATTGAAACTATATTTATA TTAATAAATTGAACTGACAAAAATAAATAAAGGAACTATATATTTTCTCAA TTATATCTTTTTACTAAAATATTATTTTTCTAATACTAGTTAAACTTTTAA AAAACATCTAATAAAGAAAAAGAATTTGTTCAATTATACTTTAGAAGCTTT TATTATTATTATTATTATTAGTAGTAGTAGTAGTAGTAATAAATTAGATTA AATTAAAGAGAGAAGTATTCAAAACTCCCAAAACTATTGTATTAGTTTTAT TTCAGAACTATTGACAATCTTAATTTTTTTTTTTTTAATTTGACTAGGTGA ACTTAAATATACTTCATTTTTTGCAAAACAAGTGAAGTACACTCTTAAATT TTCATCAAGTTTAGAAATGTTTTCAACAATTTACTAGACTCTTTATTAAGA ACTTCATGTTCTTTCAAGAGTTTATGAGCACTTGCTATGTCATGTTACAGA TCAAGAATATCTACAGAGTGTATCTAAATTTAGTACTAGTAAAGTAGAAAA TGTATTACTTATCTCTCAAACAATAGGTATTCATTATACTATTTTGAGATG TCCAACAATTTTTTTTCACTTTATGAAATCAATGAATAATTTAACACTTAG TTCCTAATTCCCAGTAAGCATTAATTATAGTTATTTACTTATTATATTTTT CAACACATTATATTGAAAAAGTGATATAGTAAATCTATCTTTTTATTTTAT TATTTCTTAAAATTTGTACAAACTTAATAATAGACAAATATTGTTGAATAG GAATAATAATTTACATTAAATCCAATATATTTTTCAATAGTTGTCACTAAA TGAAAATACTTCATCTGTTTCAATTTATGTGATAGTTTTCATTTTTCAAAA GTCAGACAATTATATATTTATAAATTAAGTAAAAAATATTATAAGTCACAC TAATTAACAATTCGAAATATTCGGTACGGAGGAACTAACACTTATGTTTTT AGACCATATTAGTCTTTTCTCTCTATTTATTATATAATATTGAGAGGAGAG TGCAACCACCATGGCAACTTTCTCTGTCTTCATAAAACGCAGCTGACATTA AAAACACAGACACACACTTCGCATTTCATATCCCTCTCACTACACGCCAAA TGCCTGCTCTTCCTATTTCTCTTCTTCTTCTTTTTCTTCTTCTCTCTCATT CACATAACACACATTCTTGTACTAACTCTGCATCATAAACTCTACCCCACT TTCTTCTTCTTCTCCGGTCATATTGCTCTGAAACTCCACTTATTGCTCTCT CCCGGCATTTATTTTTAGTTTCTCAGAAATA

Example 8 Inhibition of Nematode Infection in Transgenic Potato Plants Expressing miRNA Directed Against the StCLV2 Gene

Transgenic potato plants that expressed an artificial miRNA (amiRNA) directed against the endogenous potato StCLV2 gene (SEQ ID NO:11) were generated and assayed for both expression of StCLV2 and for resistance to G. rostochiensis infection. Two independent transgenic potato lines tested exhibited both reductions in expression of the endogenous StCLV2 gene and reductions in the numbers of G. rostochiensis (FIGS. 19 A and B).

Example 9 Soybean Nematode CLE Receptor Genes

Sequences of various candidate soybean nematode CLE receptor genes are provided in Table 5. Inhibition of the expression of such genes is anticipated to be useful in the control of nematode infections in transgenic plants. It is further anticipated that promoter sequences associated with these soybean genes will be useful in providing nematode inducible expression of operably linked sequences. Start and stop codons are underlined in the genomic and cDNA sequences provided. The soybean PNCLEPRG promoters and 5′UT of Table 5 (SEQ ID NO: 23, 26, 29, 32, 35, 38, 41, 44, 47, and 50) thus comprise the nucleic acid sequences located 5′ to the start codon of those genomic sequences.

TABLE 5 Soybean Genomic DNA sequence, cDNA sequences, and protein sequences Sequence Description DNA OR PROTEIN SEQUENCE Glyma09g29840 CAGTTCGAATCCAGGTTGCATGGAGATACAGGAAGAAACGTAAAAATTGTGTTGATACCTCAAAA gDNA and about TTAGATCAATCATTTAACTCATAGGTTGTATAATCACCTGAATTGCTTGTAATTACCATGCACAA 2.8 kb of promoter TTCCTTTAAAAATTAAACAACAAGCAAATGTTACTGTTGGAGAGCAATTCAAATTTCAAAATAAA and 5′UT Sequence TGGAACTTGTGAAAATTCAAGGAGATATTTTTAGGAATTTGTTATGTTAATTTCAAATCTTTAGA (SEQ ID ATTTTATCTAGATTTAAATATTTTATTAATTTGTTTAACTTATTTTAGGGATTTGTTTCCTTTTT NO: 23); Soybean TAAAAGATTAGAATATGATAATATTTAAATTTTGTATTGTTATTTAGCTTTATATATAGAGCCAA BAM1-like gene: GAAATACAAATTTTATAATGTGTTCCATCTAAGATTTCTTGAACGTGTGATAATTTTGTTGTGTA ATG start codon and GAAAATTTTTTCCAACGGTTAACATTTTATTAGTAGTGCTTTGCTTATAATGCAAAGAGCCTTCT TGA stop codon CCTTTATTTTATGTCTACAATAAGTAATGAATTTATAAGGAATGAAAATAACTCTTAACTCTCAA underlined GAGAGGAAAGAACTTTGGTAAACAAGATTTCATATGTTACAGCCAGACTTACACAGAATATTTCA TTTCACACAGCTCAGATGATTTTTAGAGAAAATGTACCCGATATATATTCTTCTTTTAAAGGCAG AGTTGAAATCTAAATTATATGAGCAAAATATACAACCTATACAGTATAGACAGAATCAGAAATAA AGTTCATATTTCTTAGATTACGGTATGAGAGTCACTGAGTCAATAACTTTTTACTACGAGAATAA AGAAATGGAATGATTGAATGAGCAAAATATACCCCTGAATTCCATTTTCCTAGAAAGAGAAATAG CATGCGATTGAATAAGAGAATGGCACCATCAAGATTGTGAATGAGAAAGAAGAAATGGAGGAAAC TTGTGAATGGAAAGAGAGTGAGAATGGGAGAGAGCATAGTGTTGGACAATGACATTGTGACTGTA AGGAAATTAATGAGTAACTAGAGAACGGAACGGAACTAACAAGCTTCTTGTTGTGTTTGTGATTT AAGTGTTTGATGGAGTTTTAAGGATTCAATACAATGAAAGCTACGTGACAGTTAAATATATGATA GATTCATCCTTTGAGTTCCAAGCAGTATACGTGAACGGAATCAACGTTGATCTTTAGGAAGATCA TTCTCTCCGCTCGGAAGATCTTTTATCGTTTAATCGAATCATTTTTTAAAATTTTCAGTTTTCAT TATCATGGTAAGTTTATTGATTTTTTATAATAATTTTTTTTGAAGTCATATAAAATATAATATTT TATTGATTAGAAATGTAAAATAATTTACAATAAGGGAAAATATTTATTGAACATTTTTATAATAT TAGAAATAGATTAAACTAATACAGTATTTCGGTATTGTATTGCATATATGTTTATCTATAACTAT TATTTTTAAATTATCTTTTAATATATATAACGATTTTTTTTTTATAAACTTTCAAAATGTAGATG TTACTATTTTTTCCTAAAACAATATTATCACTATTTTTTCATTTTTTTTCTTTTGAAAAAAAAAG AAAATAAAGATAAATATATGAAGTGTCTTTCTTTCAACTGGTCTTATGTAAGAACAAATTACACT CTATGCTCAGGACTTATTATACTTATACTTCCTACGTTAAAATGTATTTTTTTTATCTCTTCTAA AGTAAATTATCATCGTTTAACTTTTGAGAAAAATGTCAAAAAAAAATCCATACACTTAACTCTCA CAATCTGATTCTTCTCCATCTTTATTGGCCTCTTCTTTGTCATCCACCCTCCCGGTCAGCTAATT TTTTTGTTATAATATTATTAATATGAAATATTCATCAACTTTATCGATAAATAATTTTTATTAAA ATACTTAATTAAATATTTTTATGATGATATTTTTTTCTTTTAATTATATTTTTATTTTTCTTCAC AAGATTAAAATTTAATATCTTTCTTAACGAGATTAAATAAATATTTCATCAACATATTTTATTTT TATATATATATATTTTTTAACTCATCATATCACTTATCATATCTATATTTATTTTTATGTATCTT AATACATCATTTTAGATGGGCAAATTAAATATATTTATCCAAAAGTAATGTCATGAGAATGAGAA GAAAAGTTACATCACGCCTCCTTCTGGCCTTCTCCTAAATTATCGAGATTAATACCTTGTGCCTG TAAATTTGGTAACCAGAAAAAAGAAAAATCATGTGAGGTAGAGGATTTTTTCGAATGTGTAAAAA TAGATTTCTTGAGTGCCTAAGGTGTTTGCATTCAGCAATGGCACAACACGTGTCAAGTCCCAATC TTACAAGAACCTTCCTTCCTACCGAAAGTCCCGTCACGACACGTGAGCAGTCACATCCGTCACGT GTCACCTTTTCATCGACCATGGGAAGATCTTTCGGCACCGCACTTTCTGGTATCTTCACGCGCAA TCCCCATCCCACCGTCCATTCTCTCACACGCTCGAGCCATCGTAGCCGTCGCCCCCTCACCCGTC CCCAACTCCGCCACGCATCCAAATGACACGTGGCGCTAAAGTAACGGTCAAATCCACAATATTAC TTATTGTAACCTTATCCTCTCCTCACCCCTCACCCCCCCCCTTCCCCCTATAAATCCCCCTTTCC CTCCCTCCAATTTCAACCTCACTCTGCATTCGCTAAACCCAAAACACTATTTTATTATCTTCTTC GTCTGTTCTTTGCATTGAAGAAAATTTCTTTGAATTGAAGAAAACTTGAAATCGAATTGTGAAAC AGAAAATAAACCAAAGGAAATTTTTACTGATTGAATTGTAGAGATTGGAAAAATGGCGTTGAGTA TGACTCAACAGATCGGGACCCTAGCTGGTGCGACGGTGCCGGATTCCTCGGCCGGAGAATCGACC GCGGCGGTGAGTGCTGCCGCGGTGTGGAAGTCACCGACGGCGAGTCTGAAGTGCAAGGTAATGAG GACGGATGGCTGCGCGGAGGGGCTTTCGCCGCCGCTGAGTCCGTGCAGGTCGCCGGTGCTGCGGG CGGATCTGTCGGCGGCGTGTCAGGCATTCACGGCGGAGGTGGCGGAGGAGGAGTACGTTGCCGGA GGGAAGGAGGAGAAGGGGAAGGGGAAGGAGGGAGTGCCGGTGTTTGTGATGATGCCTTTGGACAG CGTGACGGCGGGGAACGCGGTGAACCGGAAAAAGGCGATGAACGCGGCGATGGCTGCGCTGAAGA GCGCGGGGGTGGAGGGGGTGATGATGGACGTGTGGTGGGGTTTGGTGGAGAGAGAGAAGCCTGGG GAGTATAATTGGGGAGGGTACGTGGAACTCATGGAAATGGCGAAGAAGCATGGCCTCAAGGTGCA GGCTGTTATGTCATTTCACCAATGTGGCGGTAACGTCGGAGACTCTTGCACGTGAGTCTTATGCA ATCCCTTCTTCTTCCTTCTTTTTTTCTTTTTATTTGTCATTTGTGATTTTTATTTTTACTGGCGA AATCTTATTAGATTCTAGATTAATTGGTTTTAACAATTAGAATTGTTACTAGTATTTTTTTTTAA GTTTAATTTCTGCGAATTGGTTTTGAAATCTGAAAACTAATTGAGTGACACCATGAAAAGATTTT ACGTTTTTGATACATTCTTGTTGGTTTTTTTTAACGTTAAGTTTTTGCTTTTAATTCAATTTACC ATGAAATTCACATCTTTATCTTTATTGGTAAATATGTGGTGTTATTATTATATGGTGTTTTCGTT GATTATGATTGAAAATGAGAGGCGTGCCCAGCACGGTGCAGCTCGTTTGTGAAAAATAAAATAAA CGTTTTAAAAGGGGTTTTGTGATGGGAAATGAAGCCATGCCATGTGATGTTGGACTTGTATCACT TTGATTCGAAGTATAGTATTTTTCTTTTCTATTGAATATTCAACTACGAACCTGGAATAATTGAA TCTTGAGAATTGTGTATATGATATTGATAATTATTTAGCCATTTCTCTTTAACTGAAATTTTAAT GTTTCATTTTTATTAGTACTTGAAGATTCTGAATTTAATTAAATTTTAATCCTTTTTTTACAGAA ATTAATTTTTAATCTTTGTACTATACAGAATGAGTTAACATTCTTTTATAATTAGGGATAATGAC AATTTTAATTTAGTATTTTAAACATGATGATTATATTTATTTTTATCATAATAACAACAATTTTC CTGAAAAAAAAATAAAAATAATTTCATAAATCTTTATATTATGATTTAAAGAGGCGTAATGAGCA CGGTGATGCTAGTCTTATTTTCTTTCATTTTTTGTGGTCCTTATGTAAAAAGTAAATACAAAATA CATGAGAAAAGAGTGTGCTTTCGTGATGGGAAGTGCCAAAGTGGGACCACGTGAGGATGGACTTC TAGTTCTACTGATTCACGTCGGCATCGCCACATACAGTAGACTAACTTTTAAGGACACCTTAAAT TTAGTGGACCCGATATCTTAATTTATTTTTCGGTCCATTTTTTGAAAAAGTATTCCTCAAATTCT CTCCATTTTTCTTAAAACATGTTATTCGAAACAAATAATCCAGGCATAGTTTCTGTTTATATATT TTATGTAAATTATTTTTGACAGTTATAAGATTATCTAATGGTTTCGAATTCGAATCATGGACATG TGGTAATGTTGATACTAAACAGTTGGAGGAGAGTTTAGCATCCATAATGATTCTATTCGGTTTCG AGTAGAATTATCTCTTATTAGAGATACATCTGATCTACTAAAAAATATAAATAGTTAGTGTAATT TTAGATATTACTGCCATTAATTTTGCTATAAGTTAGCACTGTGTTGGAATACCAGTTGTCTTATT GGTGGGCTTATCAGATAGTTTGTCCTGTGTTCAGTATTCCTTTGCCCAAATGGGTTGTGGAGGAG ATTGATAATGACCACGATCTTGCATATACTGATCAATGGGGAAGAAGAAACTATGAATATATATC ACTTGGATGTGATACTTTGCCGGTGCTCAAGGGACGATCCCCAGTTCAATGTTATGCTGATTTCA TGCGTGCTTTCAGAGACACTTTCAAGCACCTCCTTGGTGATACCATTGTGGTAAATATCATTCTC AGTGCACTTTTACATCATGCTGTGATTTGTTGTGCTATTTAAATATAACTTCTCATCTGAACTTC TTTTACTGGCAATATTTCAGGAAATCCAAGTTGGGATGGGACCAGCAGGTGAGTTGCGTTACCCT TCGTACCCAGAGCAAAATGGGACATGGAAATTCCCAGGAATTGGTGCTTTCCAATGCTATGACAA GGTATATATATTTATGTTTTTTTTTTCCTTCTCCTTGTTGTAGTCCTTTATATATAATTGTCTTA GGATTTGTTTGGATAAATAAATTTCTTCATGAACAAAGAGGAGAAAACAAGGTAAAATGTGTTCT AAACCTCTAATACTTAATTATGCTATGGTGCAGTATATGTTGAGTAGCTTAAAAGCTGCTGCTGA AGCTCACGGTAAGCCTGAATGGGGAAGCACAGGCCCTACTGATGCTGGCCACTATAACAACTGGC CAGAAGACACTCAATTTTTCCGCAAAGAAGGTGGTGGATGGGATGGTCCATATGGTGAGTTTTTC CTCACTTGGTACTCTCAGATGCTGTTGGAACATGGTGACAGGATTCTCTCATCAGCCACGTCGAT CTTTGACAACACTGGAGTTAAGATCTCAGTGAAGGTTGCCGGCATTCACTGGCACTATGGTACAA GGTCTCACGCCCCAGAACTCACTGCAGGGTATTACAACACCCGATTCCGTGATGGCTACCTCCCC ATTGCTCAAATGCTGGCGCGCCACGGTGCCATCTTTAACTTCACCTGTATCGAGATGCGCGATCA CGAGCAGCCACAAGAGGCCCTTTGTGCACCTGAGAAGCTGGTGAAGCAAGTGGCTCTGGCAACGC AGAAGGCACAGGTTCCACTTGCCGGCGAAAACGCGCTGCCACGGTACGACGAGTATGCACATGAG CAGATCATAAGGGCATCACAATTGGATGTTGATGGTGAGTCTGGTGATAGAGAGATGTGTGCCTT CACATACCTGAGGATGAATCCGCATTTGTTTGAACCAAATAACTGGAGGAAGTTTGTGGGGTTTG TGAAGAAGATGAAAGAAGGGAAGAGTGCACACAAGTGTTGGGAAGAGGTGGAGAGGGAAGCTGAG CATTTTGTGCATGTTACACAGCCTCTTGTGCAAGAGGCTGCAGTGCTGATGCACTGAGAATTGTT GAACATCCTTGTGGTAATAGGGCTTAGGAATAAGTCACAAGGAGGCTGTGTGAAAGTTTTAGTGA ACCAACAGCCCAGGTTTGTGGCTTTGAAGATGTAAAATTTTGTATTATATTGTTTTGTATTGTAT GCACCTAAAACTTCTATTTGTGACCCTTTTACATTGTGTACGTAATCATAGACTTTGGGGTACTG TTTCCTTAAAAGTTACTCTACTTTGTACAAGTAGTTACTTAATCTGGTTTAAAAAAATGTCATCC CTTAATCTG Glyma09g29840 ATGGCGTTGAGTATGACTCAACAGATCGGGACCCTAGCTGGTGCGACGGTGCCGGATTCCTCGGC cDNA SEQ ID CGGAGAATCGACCGCGGCGGTGAGTGCTGCCGCGGTGTGGAAGTCACCGACGGCGAGTCTGAAGT NO: 24); Soybean GCAAGGTAATGAGGACGGATGGCTGCGCGGAGGGGCTTTCGCCGCCGCTGAGTCCGTGCAGGTCG BAM1-1ike CCGGTGCTGCGGGCGGATCTGTCGGCGGCGTGTCAGGCATTCACGGCGGAGGTGGCGGAGGAGGA gene; ATG GTACGTTGCCGGAGGGAAGGAGGAGAAGGGGAAGGGGAAGGAGGGAGTGCCGGTGTTTGTGATGA start codon TGCCTTTGGACAGCGTGACGGCGGGGAACGCGGTGAACCGGAAAAAGGCGATGAACGCGGCGATG and TGA stop GCTGCGCTGAAGAGCGCGGGGGTGGAGGGGGTGATGATGGACGTGTGGTGGGGTTTGGTGGAGAG codon AGAGAAGCCTGGGGAGTATAATTGGGGAGGGTACGTGGAACTCATGGAAATGGCGAAGAAGCATG underlined GCCTCAAGGTGCAGGCTGTTATGTCATTTCACCAATGTGGCGGTAACGTCGGAGACTCTTGCACT ATTCCTTTGCCCAAATGGGTTGTGGAGGAGATTGATAATGACCACGATCTTGCATATACTGATCA ATGGGGAAGAAGAAACTATGAATATATATCACTTGGATGTGATACTTTGCCGGTGCTCAAGGGAC GATCCCCAGTTCAATGTTATGCTGATTTCATGCGTGCTTTCAGAGACACTTTCAAGCACCTCCTT GGTGATACCATTGTGGAAATCCAAGTTGGGATGGGACCAGCAGGTGAGTTGCGTTACCCTTCGTA CCCAGAGCAAAATGGGACATGGAAATTCCCAGGAATTGGTGCTTTCCAATGCTATGACAAGTATA TGTTGAGTAGCTTAAAAGCTGCTGCTGAAGCTCACGGTAAGCCTGAATGGGGAAGCACAGGCCCT ACTGATGCTGGCCACTATAACAACTGGCCAGAAGACACTCAATTTTTCCGCAAAGAAGGTGGTGG ATGGGATGGTCCATATGGTGAGTTTTTCCTCACTTGGTACTCTCAGATGCTGTTGGAACATGGTG ACAGGATTCTCTCATCAGCCACGTCGATCTTTGACAACACTGGAGTTAAGATCTCAGTGAAGGTT GCCGGCATTCACTGGCACTATGGTACAAGGTCTCACGCCCCAGAACTCACTGCAGGGTATTACAA CACCCGATTCCGTGATGGCTACCTCCCCATTGCTCAAATGCTGGCGCGCCACGGTGCCATCTTTA ACTTCACCTGTATCGAGATGCGCGATCACGAGCAGCCACAAGAGGCCCTTTGTGCACCTGAGAAG CTGGTGAAGCAAGTGGCTCTGGCAACGCAGAAGGCACAGGTTCCACTTGCCGGCGAAAACGCGCT GCCACGGTACGACGAGTATGCACATGAGCAGATCATAAGGGCATCACAATTGGATGTTGATGGTG AGTCTGGTGATAGAGAGATGTGTGCCTTCACATACCTGAGGATGAATCCGCATTTGTTTGAACCA AATAACTGGAGGAAGTTTGTGGGGTTTGTGAAGAAGATGAAAGAAGGGAAGAGTGCACACAAGTG TTGGGAAGAGGTGGAGAGGGAAGCTGAGCATTTTGTGCATGTTACACAGCCTCTTGTGCAAGAGG CTGCAGTGCTGATGCACTGA Glyma09g29840p MALSMTQQIGTLAGATVPDSSAGESTAAVSAAAVWKSPTASLKCKVMRTDGCAEGLSPPLSPCRS protein SEQ PVLRADLSAACQAFTAEVAEEEYVAGGKEEKGKGKEGVPVFVMMPLDSVTAGNAVNRKKAMNAAM ID AALKSAGVEGVMMDVWWGLVEREKPGEYNWGGYVELMEMAKKHGLKVQAVMSFHQCGGNVGDSCT NO: 25); Soybean IPLPKWVVEEIDNDHDLAYTDQWGRRNYEYISLGCDTLPVLKGRSPVQCYADFMRAFRDTFKHLL BAM1-like GDTIVEIQVGMGPAGELRYPSYPEQNGTWKFPGIGAFQCYDKYMLSSLKAAAEAHGKPEWGSTGP gene; TDAGHYNNWPEDTQFFRKEGGGWDGPYGEFFLTWYSQMLLEHGDRILSSATSIFDNTGVKISVKV AGIHWHYGTRSHAPELTAGYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQEALCAPEK LVKQVALATQKAQVPLAGENALPRYDEYAHEQIIRASQLDVDGESGDREMCAFTYLRMNPHLFEP NNWRKFVGFVKKMKEGKSAHKCWEEVEREAEHFVHVTQPLVQEAAVLMH Glyma16g34360 TATTAGCTAAACTTTGTCATAGGTTGTACGATTATAAAATATCTTTGATAGTTTCACTTATTTCC gDNA + about ATGTACAAATGTTCCTTCTAAAAGGCATGTATTAAGCGTCAAGAACTTAATTAAAAAATTGAGAA 2.7 kb promoter TTGGATAACTCGCCAGAAGCAGCCATGAATTTTAACATGAATCAGATGAGCAAGTTCCATTTCTT and 5′ UT ACTTCCCCTACATAATTGGTCCAACAAAATACATAAGAACAATAAACATAGAACTATTGTTGAGG sequence(SEQ AATCAGGAAGACAAACAATGACCATCTAATATCCTTTTAGAGTAGTAGTTGAAGATGCCAATGGC ID AGTTGACAACTAGAAGAACATGTTGAAAAGCAAACGAATAGTTCTTAATTGAGAACAAGCATCAA NO: 26) Soybean AGCACCCTCACATGATTTTTAGAGAAAATGTACCCGATATTTATTCTTCTTTTAAAGGAAGGGTT BAM1-like AAAATTTAAATTATATGAGCAAAATATAACTGTTGTTTTTTTAATAAGAGTAGGCAGAAATATTA gene; ATG AACAATAAAAGGGAGCATAAAGAAAAAAAAAATTGAGATTGCAAAGGTTTATTTTAAAAGCAGAG start codon AAAAGATAGTAACTGCTAACAAAAAGATAACATCACTCACTAACAAATCATGCCTAGAGAATAGG and TGA stop ATCAAAACTGTTTTATCCTATCAGTCAAATGACTTTTATTTTTCCTAAAAAAATAGCATAAAAGT codon CTTATCTACTGTAGTTTCAACAGTCAAATCTTAACAATAACCTTAAATTTAAGGTGGATGATGAC underlined ATTCATCCTTTGAGCTCGCAGTATAATATACCTCAACACAAGTTATTATAGACTCATTCTATGCC TTCGGAGTTCGCACTCCTAATAATTATACGCTAACGGATTCATTTATCCATCATATTTTTAAATT TCAATTTTCTAATGAAAAAATACTATAACTACTCACTTTTTATTTACACTGTGATTTAATAATAA ATTAAAAAAATATTTTTTAGATCATCATCCAATTATAATTTTTTAATGTATAATAAATTTGTTGA CTTTCATGATACTTATTTTAAAAAAATTATTAATATTGAATTCTGATTAGATGATATTAAACTCA AATAAATTATCATTTATGTTTAATTTATTGATTTTTATAATAATTATATTTAAAATTACATAAAA TATAAATTTTTATTGATTAAAAAGTGTAAAAGCTTTTTACAGAAATGGTGGATATCTATTAAACT CTTTTATAATAGAATCAAACTAATATTTTAGTACGTGAATTGAATAGAGTAAATGTTTATCTTAT AAAACTATCCTTTATAATAATAATAATAAGGCATGCCCGATATTATTATTACTATTATTGAAGGA ATATATAAGCATACGCATTTAAAAAAAATACCAAATATACTAGTTTAATTTGTAATCACAATTTT TAATCTCTAATCATCTTCAATCTAGGAATAAGTCTCTAGCTATCATATTTAAACTGAGTTTAAAA TATTTCACATATTTTGTTAATGTCAAATGACAATGTTTATTTGTTATGAAGTAATCAAAACCACG AAACAACAAAACCAAATCTAGCTCTATATTAATCACAAAATAAGTATTATATTAAAAATATCTCA AAATAAATATTATATTAATTTTTCAATGTAATATTAATTTTTCTATATTAACATCTTTGATAAGT ATCACTTTAAATTTCAATGTAATACTAAAAGTTAGATTTATAAAATTATTATTCTCTTTTATTTG TTTATTAACTTTTGTAAATAATTTATGTCAACATTTTTTAAACAAAAAAGAGTAGCTATTATATT ATACTATTTTTAAAACATCTACTTTAAAAAAGTATATCATCTATTTATTACTGGTTTAATCATGA TTGAATCACAATTGAATCATTAAAATTTAAATAAGTATCATCACTTTTTTTGTCCTACCTATTAT AGTCTGCAACTCATATTAAGTTGAATAGCTAATTTTGGGATGTGAAAAAATAGATTTCATGACCA TTGGCCGATGACATGACACTTGCCGTGTTCCCAATCTCACAAGATCCTTCTCCTCCCATATTTTC TCTTGGCTCCTACATCGACACGTGACCACACATCTCTCACGTGTCACCTTTCCATGGACCATCAC CTTCACGCGCAATCCCCATCCCACCGTCCATTCTCCCAAATGACACGTGGCGCAAAACTAACGGT CATACCCAAAATATTAATATTACTTATTGTAACCTTATCCTCACCACCCCCTTCCCCCTATAAAT ATCCTTCCCCCTCACTGCATTCGCTAAACCCAATAAATTGTTATTTTCTGTTCTTTGCATTTGAA TCAAAGCAAATTTTGATTGATTGATTAGAAAATGGCGTTGAATATGACTCACCAGATCGGGACCC TGGCTGCTGCGACGGTGCCGGTGCCGAATTCGTCTGCCGGAGAATCAACCGCGGCGATGAGTGCC GCCACTCTGTGGAAGCCGCCGGCGGTGAGTCTGAAGTGCAAGGTCACGAGGACGGAGGGCGGCGC TGAGGGGCTGTCGCCGCCGCTGAGCCCGTGCAGGTCGCCGGTGCTACGGGCGGATCTGTCGGCAG CGTGTCAGGCGTTCACGGCGGAGGTGGCGGCGGAGGAGTACATTGCCGGAGGGAAGGAGAAAGGA GAGGGGAAGGAGGGAGTGCCGCTGTTTGTGATGATGCCGTTGGACAGCGTAAAGACGGGAAACGC GGTGAACCGGAAGAAGGCGATGAACGCGGCGATGGCGGCGCTGAAGAGTGCGGGGGTGGAGGGGG TAATGATGGACGTGTGGTGGGGTTTGGTGGAGAGAGAGAAGCCTGGGGAGTATAATTGGGGAGGG TACGTTGAACTCATGGAGATGGCGAAGAAGCATGGCCTGAAGGTGCAGGCCGTTATGTCATTTCA CCAATGTGGCGGTAACGTCGGAGACTCTTGCACGTGAGTATTATTATGCAATCTCTCTCATTCTT TTTTGTCATTGCTGATTGAATGTTATTAGATTCTGGATCAATTGGTTTTAACAATTAGAATTGTT ACTATTAGATTCTGGAGTACTTTAAAGGTTTCTTTTAGGTTTAATTTCTGTGAATTCGTATTGAA ATCTGAAAATCAATTGAGTGACACCATGAAATTTTTTTACGTTTTGGAAACATTCTTATTTAAAA AAATTTTAACGTCGTGTTTTTGCTTTTAATTATATTTGTAGTTTTTTAAAATAAGCAATTATATT TTATTAGTATTAAAATTGCTGGACACGTGAAACAAAACGGCTGGATACATTCTTATTAAAAAAAT TTAACGTCAAGTTTAGATACCTAAATATTGTTATACGATATATATCTATAATGTTTGGATAATGA AATTGGTCGGACAAGCAATTTGGATGAAAATTCATGCAGTGTGAAAATGTTAATTTTTTGTGAAA GTAATTCGTTTAATTTATATTTTAATTTTTATAGTTTAAAATTAATATTTTTAGTTCTTATAATT TACATTTTAAATATTAACATATATTTTAATTAATTTCATATATTTATCTTTATAGGAAAATATGT GGTTATTAATTATATGGAGTTTTCGATGATTATGATTGAAAATGGGAGGCGTGCCCAGCACGATG CAGCCTGTTTGTGAAAAATAAAATAAACGGATAAAAGGGGTTTTGTGATGGGAAATGAAGCCAAT ACTGCCATGTGAATGATGTGATATTGGACTTGTATCACTTTGCTTCTAAGTGTAGTATTAGTTTT CTCTATTGAATGAACTAGGAACCTGGAATAATTGAATCTTGAGAATTGTGTATATTCATAATTAT TTAGCCATTTCCCTTTTACTGAAATTTTAGTGTTTCATTTTTATTACTACTATTTTGATCGAAGA TTATGAAGTTAATTAAATTTTAATCCTTGTGCTATTACGAATGAGCTGGCATTCTCTTAAAATTA GGGATAACAACAATATTAATTTAGTATTTTTAAGCATGATTATTATGCTTATTAAAAAAACATAA TTATTATATCTATTTTAACATAATAACAATGATTAAAAATAATTTCATAAATGTTTATATTTTGA TATGATTTAAAGAGGCGTAATGAGCACGGTGCAGAGTCTTATTTTCTTTCATCTTTCGTGGTCCT TGTGTGTAGTAAATACAAAATACGTGAGAAAAGAGTGTGCTTTCGTGATGGAAAGTGCCAAAGTG GGACCACGTGAGGTAGCACTTGTAGTTCTACTGATTCACGTCGGTATCGCCACAAACAGTAGACT AACTTTTTAAGGATCTACTACCTTTAATCAAGTGGACCCGAGATCTTAATTTGTTTTTCAGTCTA TTTTTTGAAAATGTATTTGTAAAATATTTTCATTTGTTTAAAATGTTATTTGAAACAAATAATCC AGATATATTTTCTGTTTATATATTTCATGTAAATTATTTCAACGGCTATCAATTATAGTAAACTA GTTTTCATTTATCAGTGATCGCATAAATCAACTATTGATTTCGAATTTGAGTCTTGGACATGCGG TAGTTAAATAGTTGGAGGAGGGTTTAAAATTCACAGTGATTCTATCTGGTTCCAGTAAGAGATAA TCCAGTAGAATTATCTCTTACAGGAGATAGCTGTGGTTTATTAAAAAAAAAAAAACTAGTTCATA TTTTTATGATTTTAGATATTATTGCCATCAGTTTTGCTGTAAGTTAGCATAGTGTTGGAATACCA GTTGTCTTATTGGTTGGCTTATCAGATTGTTTGTCTTGTGTGCAGTATTCCTTTACCCAAATGGG TTGTGGAGGAGATTGATAATGACCCCGATCTTGCATATACTGATCAATGGGGAAGAAGAAACTAT GAATATATATCACTTGGATGTGATACTTCGCCAGTGCTCAAGGGCCGAACCCCAGTTCAATGTTA TGCTGATTTCATGCGTGCTTTCAGAGACACTTTCAAGCACCTCCTTGGTGACACCATTGTGGTAA ATATCTTTCTCAGTGCACTTTTACATCATGGTGTGATTTTTGTTGCTATATAACTTCTCATCTAA ACTCCTTTTACTGGCATATTTCAGGAAATTCAAGTTGGGATGGGACCGGCAGGTGAGCTGCGTTA CCCTTCTTACCCAGAGCAAAATGGGACATGGAATTTCCCAGGAATTGGTGGTTTCCAATGCTATG ACAAGGTATATATATTTACGTTTTTTTTTCCTTCTCCTTCTTGTACTCTTTTATATATAATTGTT TTAGGATTTGTTTGGATAAATTTCTTGATGAACGAAGAGGAGAAAATTAGGTAAAATGTGTTCTA ATACTTAAATTATGCTACGGTGCAGTATATGTTGAGTAGCTTAAAAGCTGCTGCTGAAGCTGAGG GTAAGCCTGAATGGGGAAGCACAGGCCCTACTGATGCTGGACACTATAACAACTGGCCAGAAGAC ACTCAATTTTTCCGCAAAGAAGGTGGAGGCTGGGATGGTCCATATGGTGAGTTTTTCCTCACCTG GTACTCTCAGATGCTGTTGGACCACGGTGACAGGATTCTCTCATCAGCCACGTCAATCTTTGACA ACACTGGAGTGAAGATCTCAGTGAAGGTTGCTGGCATTCACTGGCACTATGGCTCAAGGTCTCAC GCCCCAGAACTCACAGCAGGGTATTACAACACCCGGTTCCGTGATGGCTACATCCCCATTGCTCA AATGTTGGCACGCCACGGTGCCATCTTCAACTTCACCTGTATTGAGATGCGCGATCACGAGCAGC CACAAGATGCCCTTTGTGCACCCGAGAAGCTTGTGAAGCAAGTGGCTCTGGCAACGCAGAAGGCA CAGGTTCCACTTGCTGGTGAAAATGCGCTGCCACGGTACGATGAGTATGCTCATGAGCAGATCAT AAGGGCATCACAGTTGGATGTTGATGGTGACTCTGGTGGAAGAGAGATGTGTGCATTCACTTACC TGAGAATGAACCCGCATTTGTTTGAACCAAATAACTGGAGGAAGTTTGTGGGGTTTGTGAAGAAA ATGAAAGAAGGGAAGAGTGCACACAAGTGTTGGGAAGAGGTGGAGAGGGAAGCTGAGCATTTTGT GCATGTTACACAGCCTCTTGTGCAAGAAGCTGCAGTGCTGATGCACTGAGAATTGTTGAACAATC TTGTGCTGATAGATGGCTTAGAAAAGGTCACAAGTAGGCTGTGTGAAAGTTTTAGTGAACCAGCA GCCCAGGTTTGTGGCTTTGAAGATGTAAAATTTTGTATTATATTGTTGTTTTATATTCTATGCAC CTAAAACTTCTATTTGTTACCCTTTTATATTGTGTACGTAATCATTGACTTTGGGGTACTATTTT CTTAAAAGTTACTCTACTTTGTACAAGTAGTTACTTATTTCTGCATCATGAAACTGTTACATGGC GTAACAGCAACAAGAGATGCTATTTTCTTCTATAGGGAAAAATGAATTTAAAATCAATGATTTTC GTTGTGTTT Glyma16g34360 ATGATGCCGTTGGACAGCGTAAAGACGGGAAACGCGGTGAACCGGAAGAAGGCGATGAACGCGGC cDNA (SEQ ID GATGGCGGCGCTGAAGAGTGCGGGGGTGGAGGGGGTAATGATGGACGTGTGGTGGGGTTTGGTGG NO: 27) Soybean AGAGAGAGAAGCCTGGGGAGTATAATTGGGGAGGGTACGTTGAACTCATGGAGATGGCGAAGAAG BAM1-like CATGGCCTGAAGGTGCAGGCCGTTATGTCATTTCACCAATGTGGCGGTAACGTCGGAGACTCTTG gene; ATG CACTATTCCTTTACCCAAATGGGTTGTGGAGGAGATTGATAATGACCCCGATCTTGCATATACTG start codon ATCAATGGGGAAGAAGAAACTATGAATATATATCACTTGGATGTGATACTTCGCCAGTGCTCAAG and TGA stop GGCCGAACCCCAGTTCAATGTTATGCTGATTTCATGCGTGCTTTCAGAGACACTTTCAAGCACCT codon CCTTGGTGACACCATTGTGGAAATTCAAGTTGGGATGGGACCGGCAGGTGAGCTGCGTTACCCTT underlined CTTACCCAGAGCAAAATGGGACATGGAATTTCCCAGGAATTGGTGGTTTCCAATGCTATGACAAG TATATGTTGAGTAGCTTAAAAGCTGCTGCTGAAGCTGAGGGTAAGCCTGAATGGGGAAGCACAGG CCCTACTGATGCTGGACACTATAACAACTGGCCAGAAGACACTCAATTTTTCCGCAAAGAAGGTG GAGGCTGGGATGGTCCATATGGTGAGTTTTTCCTCACCTGGTACTCTCAGATGCTGTTGGACCAC GGTGACAGGATTCTCTCATCAGCCACGTCAATCTTTGACAACACTGGAGTGAAGATCTCAGTGAA GGTTGCTGGCATTCACTGGCACTATGGCTCAAGGTCTCACGCCCCAGAACTCACAGCAGGGTATT ACAACACCCGGTTCCGTGATGGCTACATCCCCATTGCTCAAATGTTGGCACGCCACGGTGCCATC TTCAACTTCACCTGTATTGAGATGCGCGATCACGAGCAGCCACAAGATGCCCTTTGTGCACCCGA GAAGCTTGTGAAGCAAGTGGCTCTGGCAACGCAGAAGGCACAGGTTCCACTTGCTGGTGAAAATG CGCTGCCACGGTACGATGAGTATGCTCATGAGCAGATCATAAGGGCATCACAGTTGGATGTTGAT GGTGACTCTGGTGGAAGAGAGATGTGTGCATTCACTTACCTGAGAATGAACCCGCATTTGTTTGA ACCAAATAACTGGAGGAAGTTTGTGGGGTTTGTGAAGAAAATGAAAGAAGGGAAGAGTGCACACA AGTGTTGGGAAGAGGTGGAGAGGGAAGCTGAGCATTTTGTGCATGTTACACAGCCTCTTGTGCAA GAAGCTGCAGTGCTGATGCACTGA Glyma16g34360 MMPLDSVKTGNAVNRKKAMNAAMAALKSAGVEGVMMDVWWGLVEREKPGEYNWGGYVELMEMAKK protein(SEQ ID HGLKVQAVMSFHQCGGNVGDSCTIPLPKWVVEEIDNDPDLAYTDQWGRRNYEYISLGCDTSPVLK NO: 28) Soybean GRTPVQCYADFMRAFRDTFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWNFPGIGGFQCYDK BAM1-like gene YMLSSLKAAAEAEGKPEWGSTGPTDAGHYNNWPEDTQFFRKEGGGWDGPYGEFFLTWYSQMLLDH GDRILSSATSIFDNTGVKISVKVAGIHWHYGSRSHAPELTAGYYNTRFRDGYIPIAQMLARHGAI FNFTCIEMRDHEQPQDALCAPEKLVKQVALATQKAQVPLAGENALPRYDEYAHEQIIRASQLDVD GDSGGREMCAFTYLRMNPHLFEPNNWRKFVGFVKKMKEGKSAHKCWEEVEREAEHFVHVTQPLVQ EAAVLMH Glyma01g40590 TTGAGAACTTAACCTACTAAAATTATTCTTTGATGTAATGTTAATGATTTTTTTATTTATAATTA gDNA + about TTCTAATTTAAATATGCATCTACTAGTATATTCTAATTTTACTCCCCAACATAAAAAAGTCTAAT 5 kb upstream TTATCTATTTTCTCTCTCAAATCCCTTTACAAAACTAAAATAGTAAATTGCATTAAAAATATAGA promoter and TGTATAACATGCTAAAGAAAAATTAATGTTTCCCCATGTTACCCCTAAAACTTATCATGCAAATG 5′UT sequence GATGATCAAGTCATAAGAAATGTAATATTCATAAATAGATAAGAAGATAAATTACATCAAAAGTA (start and GTTGACGGTCAAATTTTCAACAAAAAAGGTTTAGCCTCTTATTGTCATGGAGATTTTATAATTGC stop codons AAGAGTAAAATATTTAGTAAAGGGGAGAAAATAAAAAAGGGAATAAAGGAAATGAATGACTCTCA underlined; ATATTTATTTCTCCTTCTTCTAGTCTTTGCCTTCTATAATGAAGTGTATTCTCTCTTAAAAATTT SEQ ID NO: 29); TCCTTTGTTTTTTCTTATTCTCTCCTTTTCTTTTATAGATGCATATTAGTGGGCTTCTTGCATTA Soybean BAM2- AGTCTAAGTCTGTCTTTATTTTTCTTAATTAGTCATATTTTTCTTAATTAGTTCGCTTTCCTTAA like gene TTATTCCTCTCTTCTTGAATTATCCTACTTTTTTTTTACTCACTAAGCATAATAAATTCATCATT TTTAATATTTGTTGCACAAAAAATAAAATAATGTTAATTTAACAATTATTTGCTTAAAAAAAATT AGAAGAAAAAAATTACAAATTCTTATATATTTTAACCCTCAAAATATACTTATAATTAGTTGTTA TTGATTTTAAAGTTAACCTATTTTTTCAAGATATCCATGGTAGGTATTTTCAAATTACACACTTC ACATGTAAACTTTGAGGTTGCAAGGGTGAAAACAGGTAAAAAGAATAACAGCTAGCAAAGACATT TAAAATAATTCTAGCAATATAAGTCCAATCTAAAGCGGATACGTCCAGCAATATTCATCCCTCAC CAACTCCAACTTCACTCTCAATAAACTGGAAAATTATAACCAAACATGCTGAATCGTGAAGGCAT CCCTACAATTCCTTCCTAGCCAACCAGCCCAACAATTTTCTTAGCTTTTAGAAATATTATCGTGT GCAATGTGATACACTGCAGTAAGCATCAACAAGAATAGTAACCTGACCTTTCATGCCATATATGA TCGAAGTGGTCAAGAATGGCAAGTAGAAGTGAGGTTCATGCTCTTTAATGATTAATCTAATGGGA TAAAAAGGACAAAGACAAACAGAACTCTTAATAGAAAAGAAAAAAACTAAGTGGGTCAACAATGC ATATTTTGGATTCAAAACCACCACTGTCCAATCGACAACATTGTTCTACAAAACCGGAATGATTG TGATTCATCCGGAGGGTATTTGCTCATTCATGTTCCTTATTGTCGATATGGGCATGCCTAACTAG CTAAGTACAATTTCCTTAATTTCTATTTTGGCACTTACAATCGTAATTAAAACTGAAATCAGGTT TATATATATATATATATATATATATATATATATATATATATATATATATATATATAAATTAGCAT GCATTATATATTTAAGGGGTACGGGTAACGTGTGTACAATATACTCCTTACAAAAGGTTTATATC TCTGCTCGGCTTCTTATCCCAAAATTAGCAAGCATTAAATGAAGGGTAACGTGTGTTTTGTTCTT ATTAAAAAAAAAACATAGTACAATTTTTTAAGTGGAAACATGGAAATATTTTTCACTCTTTTTAA TGATTTTTTTATAACATAAAATTAAAATATTAATTCTAAAGTAGCCAATAATTATAAATTTTTCA CTAACTATGTATTGTAATGAAAAAAAATATTTTTATATTTTACTTTTGGAAAATTTTAAATTTAT TTGTTAGCAAATGATCTGTTCATGATATATTTTTATTAATTTTAAATATTATAATTTAAAACATA TATATTTAAATTTAATTTTCTGATACAACATTGGAGGATTATATATATAACTGCTCAGATAGACC CCTCCAATAGTCCAATTGTAATAAGAGTTTGAGAACATAAGAAAAAAAATCTTGTAATTACTAAT CTATATTGTGGCTTTCCTCATACAATTGATCCATGGAGAGAAGGAGTAATTCACAATAATAATAG TAATTATTAGTATTATAATAAATGTTAATGTTGGTGACTGCTTGGTCATTTTCTCTTCCAGAAAA ACAGATAAGCTGTGACCTGTTAGTAAGGCCATGGTGGGAGGGACCACTGCATGGCATCTTTCTCA GTGCTACTAGTGCTTCACTTATTACATGATTTTGAAGTTGTCAGTGAGCGGGTAGAAGATGGAGG CCATGGTCCACACTTTGTTGCCGCATTGCAAGAAAATGGTAAAAATGATATTGAATCTGCAACCC CCCAATGTAAGGGCCTCTTGTAATAATGGAAGCAGCACAGGGGCGAAGTCACACATTGATAATAG GGTTTATCGAAAACACCACATCACACCATACCACTTCACTTACCACGCCCCCTCTCTTTTCGTGT CAACAATCTTTGACCACCTTTATCCAACCTAACAAAATCATTACTGTTTATTAATTTTATACTCT TGTTTTACTAGTAATTTTCTATATTGATTTCGTTCATTTGTTATGCAGGTGTGAAAATGAACACG ATCAATAAAAGAAAGGAAGAAAAATCTAGCCTTTAGTGATGATATCGGACTTCTTTTTTGTTTTT TCAAAAGGAGGGCTTGCAATTCGACAATAACTAAGCAAAATTAACAAAAATTAAAGAAACAATAA TCCATTTTCTGTCATAATTTCGTGCTTTGATAAATTTAATACTGCAATATTATTGTAGAACCCGT GATTATGAAGTATAAGAACATAAACTTCATGTGATAAATTTTCACTGCAAATAGAATGTCTATAT GTTTTTCATTTAAGACACACTATTACAAAAAACAATCTTTGAACGACGATTCATTGACACATTTA ATAATTGTTTTTAACCGTTATTGAAGTGAATGTAATGAGAAATATTATATTTTTTACGATAATTT CTTAATCATCTTAGAAGATCTCATCTTTTAAGATAACTTTTATGTTAAAACCGTTGTAGAAGACC CACCATCCTAAAAGAACACTAACTAGAAAAAGAATGGTGGAGAGGGTGAAATAGCTACGGGTTCC TTGGCTTAGTGTACAGTTTGGCGGGACCTTACTCCTTGGGAAGGCTAGACAGTAGAAGGATACTC CGAGATCACTTCAAAGAGAATACGACACCCATGATTATCAAAAGGTTAGACAAGTTGATGCGCAA TTTCCTTGTTTTCAGTCATAATTTTGGACTAATTAAACTCCACACAGAACGACAAGCATGCTTAT TTTCTAGGCTTTTGCTTTGCTGAATACTAGAAGATAAATCTCATAGCTTTAGCCCATTGCCAAAC GCTGGATTTTACTCTCTTCCTCACAAGATGGTAACAAGTTAGATAATCTAAGATTTGTGACCTTA TTCGTCTTATGTTTGGGTTAATATTCATGTTGTACCGAGTATCATGTGCTCTAAAACATGCAGTT TTGGCTTGGCAATGAATTAGAAGTATTCCATCAAAGTAATTCATACCATACCCCATTTTTAAAGC TCAAAATGAGCAAGATAAAAACTTTAAACGTATCTTAGGGCATTCATTATTATCAAAAGCCTTTA TATTCATTAGAACTCTTTGCATGTATAGACCATTTTCTCTTTTTTAAATAAAAAACATATTAACA TATGTATCTCAGGGAATTTATTAAACAATTAAAAATGAAAATATTTATATAAAAATATTATCGAC ATAATATTATTATTATTATTATTATTATTATTATTATTATTATTATATATCGTGAGTTTTAATTA AAAAAAATTCATTGATACTCTTTAAAGTAGAAACGCTTGTTAGTAAAAATGATATTTTTGAATTT AAAAGGTTATACATTTTTATATTATTGTTAAAATTTAAAACTTAATAATGAAAATTAAAAATATT TATTTTTATCCTCAAATGACTAGACACTACAACAAAATAAATAATAAATAAGACAAGGAAAACTA ACAAAAGAACTAACCGTTGTCCTTGACCTTCCTTGGAAAATAAGGCAATAGCATAGGACCTACTT CAAAAAAGACATTCGACTACAAAAACATGCAAAATGGACAAAGATGAACAGAAAAACTAAGAAGA CGTTGCATTTATTTTTTCAATTTCACGTATTTTCATTGAAAATTATATTTTAACATTATTCATTT ATTTGTTAACAGGCCTATTTTAAAATTCGAAACCTCGGTATTTTATTAAACTCATTAAAATATCT ACACCATTTTTTATTAAAAATATAATAATAATAATTTTAATATAGTTTCTTAATAATAAAATCTC TAATAACTGCGAAAAAAGTATTTTTCTAAAAATACCATAATTAAATACGTACAACAACGAAGTAT TAAACATATAAAACTAAAGAACCACGACACATTTATGTCTTTCCTATCACAATCATAAGTAATGC TTGATTTGTGAGCACACTCTCCATAACCAACAACACACACATAACATTCTTTTATTAAAATCATT TTAAATTATGTCACATAATAACTACTGTAACAACACACATTAGCATGAAACTGGTATTAGTAGCA CATACAATAAATAAATATTGATTATTATCTGATGTAATTATGTAAGTATTATGAGTGGTTGATTA AAAAAACAAAATAGAGTTGGTAAGGGGGTGGATCCACATCCACCGCTTCTGCACCAAACTCAGCA TAGCAGTGGGTCAATGATTGATTGGTAATTGTAATTCTATTCAAAAAGTGAAAAGAGTTGAATGA GAATTCGTATATTCAGAAAATCCCCCCTCCTTTAAGATAAGAGAATAGGCCTCACTCTTTCTTTC TCTTCCATTCCCAAAATGCGTGTCCTCTTTCTTTTTCTGTTTTTCCAGTTTCTCCATTTTCATTT CCCCAAAACCCTTTCAGCCCCAATCTCAGAGTACCGTGCCCTTCTCTCTCTCCGTTCAGCCATTA CCGACGCCACCCCACCTCTTCTCACTTCGTGGAACTCCTCCACCCCTTACTGTTCCTGGCTCGGC GTCACCTGCGACAACCGCCGCCACGTCACCTCCCTAGACCTCACCGGCCTCGACCTCTCCGGCCC CCTCTCCGCCGACGTCGCCCACCTCCCATTCCTCTCCAACCTCTCCCTCGCCTCGAATAAGTTCT CCGGCCCCATTCCTCCCTCACTCTCCGCTCTCTCCGGCCTCCGCTTCCTCAACCTCTCCAACAAT GTCTTCAACGAAACCTTCCCCTCGGAGCTCTCGCGCCTCCAGAACCTCGAGGTCCTCGACCTCTA CAACAACAACATGACCGGCGTGCTTCCCCTCGCCGTCGCGCAGATGCAGAATCTTCGTCATTTGC ATCTCGGCGGCAACTTCTTCTCCGGCCAGATCCCGCCGGAGTATGGACGCTGGCAGCGCCTCCAG TACCTCGCCGTCTCCGGCAACGAGCTCGAGGGGACTATCCCTCCGGAGATCGGAAACTTGTCCAG CCTCCGGGAGCTCTACATCGGCTACTACAACACCTACACCGGGGGCATTCCGCCGGAGATCGGAA ATTTGTCGGAGCTGGTGAGGCTCGACGCCGCCTACTGTGGGTTGTCCGGCGAGATTCCGGCGGCG CTGGGAAAGCTTCAGAAGCTGGACACGCTGTTCCTTCAGGTGAATGCATTGTCAGGGTCTTTGAC TCCCGAGCTGGGGAACCTGAAGAGCCTGAAATCCATGGATTTGTCTAACAACATGCTCTCCGGTG AGATTCCGGCGAGATTCGGCGAGCTGAAGAATATTACTCTTCTGAATCTGTTCAGGAACAAGCTT CACGGAGCTATACCAGAGTTTATAGGGGAGCTTCCAGCGTTGGAAGTTGTGCAACTGTGGGAGAA TAACTTCACAGGTAGCATTCCAGAGGGTTTGGGCAAAAACGGGAGACTCAACCTTGTTGATCTTT CTTCTAACAAGTTAACTGGGACTTTGCCTACTTATCTCTGTTCTGGGAATACTCTTCAGACTCTG ATAACTCTTGGGAATTTTCTTTTTGGTCCAATTCCTGAGTCGCTTGGTAGTTGTGAATCCCTTAC ACGGATTAGAATGGGAGAGAACTTTTTGAATGGTTCCATTCCGAGAGGGCTTTTTGGACTTCCCA AACTAACACAGGTTGAGCTTCAGGATAATTATCTCTCTGGAGAGTTTCCTGAGGTGGGTTCTGTT GCTGTTAATCTTGGTCAGATTACTCTCTCTAACAACCAGCTTTCTGGGGTTCTACCTCCCTCCAT TGGTAACTTCTCCAGCGTGCAGAAGCTCCTTCTTGATGGCAACATGTTCACGGGTCGGATACCTC CCCAGATTGGGAGGTTGCAACAGCTTTCTAAGATTGATTTTAGTGGCAACAAGTTCTCGGGTCCT ATTGTGCCTGAGATCAGTCAGTGTAAGCTGTTAACTTTCCTTGACCTTAGCCGCAATGAGCTATC TGGAGACATCCCAAATGAGATAACTGGCATGAGGATATTGAATTACTTGAATCTTTCTAGGAATC ATTTAGTGGGTGGCATTCCCTCTTCGATATCATCTATGCAAAGCTTGACTTCTGTTGATTTTTCA TACAACAACCTGTCTGGTTTGGTGCCTGGTACCGGTCAATTCAGCTACTTCAATTACACGTCTTT CTTGGGAAACCCTGACCTCTGTGGCCCCTATTTGGGTGCTTGCAAGGATGGGGTTGCCAATGGCG CACACCAACCTCATGTTAAAGGTCTCTCCTCTTCTTTTAAGCTGCTACTTGTTGTTGGGTTGCTA CTATGTTCCATTGCTTTTGCTGTGGCTGCAATATTCAAGGCCCGGTCACTGAAGAAGGCCAGTGG GGCTCGTGCATGGAAGTTGACTGCGTTCCAACGTTTGGACTTCACTGTCGATGATGTTTTGCATT GCTTGAAGGAGGATAATATTATAGGGAAAGGAGGTGCTGGCATTGTCTACAAAGGGGCTATGCCT AATGGGGATCATGTTGCTGTGAAAAGGCTTCCGGCTATGAGTAGAGGCTCTTCACATGATCATGG CTTCAATGCTGAGATTCAAACATTGGGGCGAATCCGACACAGGCACATTGTTAGGTTGTTGGGCT TCTGTTCAAATCATGAGACAAACCTTTTGGTCTATGAGTACATGCCCAATGGAAGTTTAGGCGAG GTTCTTCATGGAAAGAAAGGGGGTCATTTGCATTGGGATACAAGGTATAAAATTGCGGTGGAGGC TGCCAAGGGGCTTTGCTATCTGCACCATGATTGTTCGCCACTCATTGTCCATCGTGATGTGAAGT CAAACAACATCCTTCTTGATTCTAATCATGAAGCCCATGTTGCTGATTTTGGGCTTGCTAAGTTC CTGCAAGATTCTGGGACATCTGAATGCATGTCTGCTATTGCTGGTTCATATGGATACATAGCTCC AGGTACCGTCCAATTTCGACATAATTAATGCATTATTTACATGGTTGTGGAAAATTTTCTTTTAC CCGCCTGTTCATAATTGTACGTTTAATCATTGTTCAGAATTTGACTCTTTGACTTATCATCATGT TTTAGGTGTAGACTGTTGATATTGAGGTGATGTCCCTAAATTAATTAACATTGCTATGTGGTTTT TCTTGACTTTGGTTTTCTATCATACCCAAATGATCTCTTGATTTCGACCCCTTATTTAGTCTATT TCAAGCCAAGTACTGAAAGTAAATGGTAGATAGCTCTGCAACGTTAGAGTCATTCACGACCGGAA ACTGATGATTATGGGCAAAATATCGGATAAAAAGACCTATTATGTTACTTTACACTTATTGCCTT TGTTTAACTTATAGTTTCAAATTCAAGTGTCTTGCTTTATTTTAGTTTATGATACATGTTCGATG TTTGATTGCAGAGTATGCCTACACATTGAAAGTTGATGAGAAAAGCGATGTGTACAGTTTTGGTG TGGTTCTCTTAGAACTTATAACAGGCAGGAAACCAGTTGGAGAATTTGGTGATGGCGTGGACATA GTGCAATGGGTGAGGAAAATGACGGATTCTAACAAGGAAGGAGTTCTTAAAGTTCTTGATCCTAG ACTTCCCTCAGTTCCCCTTCACGAAGTGATGCATGTTTTCTATGTAGCCATGCTGTGCGTTGAAG AACAGGCTGTAGAGAGACCAACTATGCGTGAAGTTGTTCAAATACTGACAGAGCTTCCAAAGCCA CCTGACTCTAAAGAGGGGAACTTAACAATAACGGAATCATCTTTGTCATCATCAAACGCTTTAGA ATCTCCATCCTCAGCCTCTAAGGAAGATCAAAATCCTCCTCAATCCCCACCACCCGATCTTCTTA GCATTTAAAGTGCTCTGTTGGGTGTTTCATCTTAGTTCCCTTGGGTTGTGATCGCTTATCCATTT ACTTTCTTTTTCTGTCTCTCTTCTGGGATTGGTTTTTTTTTTTTTCCCTAACTGAAGGTGTTAAT GTTTGGATTTTTTAATGGTTTTGTACAGTAGGATTGATGGGGGTATTTTCTTATAAAGTCACTGT CTTCATCATGTAGTACTGCTTTTTAATTTTTATTTGCGACCGTTGTTGGGGAGGATTCAAGGGAT ACAATTAAATTACTCGTTTGTTTCCTGAAATTTCATTATTCATACTTTTTTAGTTTATG Glyma01g40590 ATGCGTGTCCTCTTTCTTTTTCTGTTTTTCCAGTTTCTCCATTTTCATTTCCCCAAAACCCTTTC cDNA (start and AGCCCCAATCTCAGAGTACCGTGCCCTTCTCTCTCTCCGTTCAGCCATTACCGACGCCACCCCAC stop codons CTCTTCTCACTTCGTGGAACTCCTCCACCCCTTACTGTTCCTGGCTCGGCGTCACCTGCGACAAC underlined; CGCCGCCACGTCACCTCCCTAGACCTCACCGGCCTCGACCTCTCCGGCCCCCTCTCCGCCGACGT SEQ ID NO: 30); CGCCCACCTCCCATTCCTCTCCAACCTCTCCCTCGCCTCGAATAAGTTCTCCGGCCCCATTCCTC Soybean BAM2- CCTCACTCTCCGCTCTCTCCGGCCTCCGCTTCCTCAACCTCTCCAACAATGTCTTCAACGAAACC like gene TTCCCCTCGGAGCTCTCGCGCCTCCAGAACCTCGAGGTCCTCGACCTCTACAACAACAACATGAC CGGCGTGCTTCCCCTCGCCGTCGCGCAGATGCAGAATCTTCGTCATTTGCATCTCGGCGGCAACT TCTTCTCCGGCCAGATCCCGCCGGAGTATGGACGCTGGCAGCGCCTCCAGTACCTCGCCGTCTCC GGCAACGAGCTCGAGGGGACTATCCCTCCGGAGATCGGAAACTTGTCCAGCCTCCGGGAGCTCTA CATCGGCTACTACAACACCTACACCGGGGGCATTCCGCCGGAGATCGGAAATTTGTCGGAGCTGG TGAGGCTCGACGCCGCCTACTGTGGGTTGTCCGGCGAGATTCCGGCGGCGCTGGGAAAGCTTCAG AAGCTGGACACGCTGTTCCTTCAGGTGAATGCATTGTCAGGGTCTTTGACTCCCGAGCTGGGGAA CCTGAAGAGCCTGAAATCCATGGATTTGTCTAACAACATGCTCTCCGGTGAGATTCCGGCGAGAT TCGGCGAGCTGAAGAATATTACTCTTCTGAATCTGTTCAGGAACAAGCTTCACGGAGCTATACCA GAGTTTATAGGGGAGCTTCCAGCGTTGGAAGTTGTGCAACTGTGGGAGAATAACTTCACAGGTAG CATTCCAGAGGGTTTGGGCAAAAACGGGAGACTCAACCTTGTTGATCTTTCTTCTAACAAGTTAA CTGGGACTTTGCCTACTTATCTCTGTTCTGGGAATACTCTTCAGACTCTGATAACTCTTGGGAAT TTTCTTTTTGGTCCAATTCCTGAGTCGCTTGGTAGTTGTGAATCCCTTACACGGATTAGAATGGG AGAGAACTTTTTGAATGGTTCCATTCCGAGAGGGCTTTTTGGACTTCCCAAACTAACACAGGTTG AGCTTCAGGATAATTATCTCTCTGGAGAGTTTCCTGAGGTGGGTTCTGTTGCTGTTAATCTTGGT CAGATTACTCTCTCTAACAACCAGCTTTCTGGGGTTCTACCTCCCTCCATTGGTAACTTCTCCAG CGTGCAGAAGCTCCTTCTTGATGGCAACATGTTCACGGGTCGGATACCTCCCCAGATTGGGAGGT TGCAACAGCTTTCTAAGATTGATTTTAGTGGCAACAAGTTCTCGGGTCCTATTGTGCCTGAGATC AGTCAGTGTAAGCTGTTAACTTTCCTTGACCTTAGCCGCAATGAGCTATCTGGAGACATCCCAAA TGAGATAACTGGCATGAGGATATTGAATTACTTGAATCTTTCTAGGAATCATTTAGTGGGTGGCA TTCCCTCTTCGATATCATCTATGCAAAGCTTGACTTCTGTTGATTTTTCATACAACAACCTGTCT GGTTTGGTGCCTGGTACCGGTCAATTCAGCTACTTCAATTACACGTCTTTCTTGGGAAACCCTGA CCTCTGTGGCCCCTATTTGGGTGCTTGCAAGGATGGGGTTGCCAATGGCGCACACCAACCTCATG TTAAAGGTCTCTCCTCTTCTTTTAAGCTGCTACTTGTTGTTGGGTTGCTACTATGTTCCATTGCT TTTGCTGTGGCTGCAATATTCAAGGCCCGGTCACTGAAGAAGGCCAGTGGGGCTCGTGCATGGAA GTTGACTGCGTTCCAACGTTTGGACTTCACTGTCGATGATGTTTTGCATTGCTTGAAGGAGGATA ATATTATAGGGAAAGGAGGTGCTGGCATTGTCTACAAAGGGGCTATGCCTAATGGGGATCATGTT GCTGTGAAAAGGCTTCCGGCTATGAGTAGAGGCTCTTCACATGATCATGGCTTCAATGCTGAGAT TCAAACATTGGGGCGAATCCGACACAGGCACATTGTTAGGTTGTTGGGCTTCTGTTCAAATCATG AGACAAACCTTTTGGTCTATGAGTACATGCCCAATGGAAGTTTAGGCGAGGTTCTTCATGGAAAG AAAGGGGGTCATTTGCATTGGGATACAAGGTATAAAATTGCGGTGGAGGCTGCCAAGGGGCTTTG CTATCTGCACCATGATTGTTCGCCACTCATTGTCCATCGTGATGTGAAGTCAAACAACATCCTTC TTGATTCTAATCATGAAGCCCATGTTGCTGATTTTGGGCTTGCTAAGTTCCTGCAAGATTCTGGG ACATCTGAATGCATGTCTGCTATTGCTGGTTCATATGGATACATAGCTCCAGAGTATGCCTACAC ATTGAAAGTTGATGAGAAAAGCGATGTGTACAGTTTTGGTGTGGTTCTCTTAGAACTTATAACAG GCAGGAAACCAGTTGGAGAATTTGGTGATGGCGTGGACATAGTGCAATGGGTGAGGAAAATGACG GATTCTAACAAGGAAGGAGTTCTTAAAGTTCTTGATCCTAGACTTCCCTCAGTTCCCCTTCACGA AGTGATGCATGTTTTCTATGTAGCCATGCTGTGCGTTGAAGAACAGGCTGTAGAGAGACCAACTA TGCGTGAAGTTGTTCAAATACTGACAGAGCTTCCAAAGCCACCTGACTCTAAAGAGGGGAACTTA ACAATAACGGAATCATCTTTGTCATCATCAAACGCTTTAGAATCTCCATCCTCAGCCTCTAAGGA AGATCAAAATCCTCCTCAATCCCCACCACCCGATCTTCTTAGCATTTAA Glyma01g40590 MRVLFLFLFFQFLHFHFPKTLSAPISEYRALLSLRSAITDATPPLLTSWNSSTPYCSWLGVTCDN protein (SEQ ID RRHVTSLDLTGLDLSGPLSADVAHLPFLSNLSLASNKFSGPIPPSLSALSGLRFLNLSNNVFNET NO: 31); FPSELSRLQNLEVLDLYNNNMTGVLPLAVAQMQNLRHLHLGGNFFSGQIPPEYGRWQRLQYLAVS Soybean BAM2- GNELEGTIPPEIGNLSSLRELYIGYYNTYTGGIPPEIGNLSELVRLDAAYCGLSGEIPAALGKLQ like gene KLDTLFLQVNALSGSLTPELGNLKSLKSMDLSNNMLSGEIPARFGELKNITLLNLFRNKLHGAIP EFIGELPALEVVQLWENNFTGSIPEGLGKNGRLNLVDLSSNKLTGTLPTYLCSGNTLQTLITLGN FLFGPIPESLGSCESLTRIRMGENFLNGSIPRGLFGLPKLTQVELQDNYLSGEFPEVGSVAVNLG QITLSNNQLSGVLPPSIGNFSSVQKLLLDGNMFTGRIPPQIGRLQQLSKIDFSGNKFSGPIVPEI SQCKLLTFLDLSRNELSGDIPNEITGMRILNYLNLSRNHLVGGIPSSISSMQSLTSVDFSYNNLS GLVPGTGQFSYFNYTSFLGNPDLCGPYLGACKDGVANGAHQPHVKGLSSSFKLLLVVGLLLCSIA FAVAAIFKARSLKKASGARAWKLTAFQRLDFTVDDVLHCLKEDNIIGKGGAGIVYKGAMPNGDHV AVKRLPAMSRGSSHDHGFNAEIQTLGRIRHRHIVRLLGFCSNHETNLLVYEYMPNGSLGEVLHGK KGGHLHWDTRYKIAVEAAKGLCYLHHDCSPLIVHRDVKSNNILLDSNHEAHVADFGLAKFLQDSG TSECMSAIAGSYGYIAPEYAYTLKVDEKSDVYSFGVVLLELITGRKPVGEFGDGVDIVQWVRKMT DSNKEGVLKVLDPRLPSVPLHEVMHVFYVAMLCVEEQAVERPTMREVVQILTELPKPPDSKEGNL TITESSLSSSNALESPSSASKEDQNPPQSPPPDLLSI Glyma11g04700 GTTGGAGTAAATCCAATAACATCAAATCCTTAATATATATTTATTAAATTTTATTGATAAAACTG gDNA + about ACTTACTAGTACATATTTTAGTTTGTAATAATATCATTTGTTTGGATCCAATATATAAGCCAATT 5 kb promoter TTTTTTATGGACAAAATATATGGAGCCAAAGCCGCAGCTCAAAAACCTATGTAACAAGAGACACT and 5′UT GAAGAGTGAAGAATCAGCAACATGATCAAAGCCTAAAATTGGGGCAAAAATTCAAACACTTGGCT sequence (SEQ ATAAATACACCAGATAGTCCATACTTAGCCGCTATTATGTCAAAATATAATAGTATTAATATTAC ID ATGGCAAAGTATAGGCTATATAATTTAATGTAATTTATTAAATTTTACAAGGTACTGATTCAACT NO: 32) Soybean TTAAACATGTATGCTAATTGGAGTTTAAAATTTGTGAACAAAAAGCAAGTGCATTTTGTTGCGTG BAM2-like gene ATCAAAATTGCTCAACCTTATCATGTAGGAAAACGGATAACCAGAATTTGTGTGGTCCCAAACGA CAACAAGACGCATTTATAAGCTTGACTAGTTCTCTTCGTCGTCAACTGACATTCTCATTTCTCAA TGATAGTTGCTACTTGATAATATTTTATTCGAATAATCTGTCGTTAACCTACCTATAATATATAG CTGGTGCTATTAATCGAATGTTTAATCTCATTTTAAGATTTACAGTGTGTGGATTGATGGTGAAG ATCCAAAAATCATAGTATCTGATTATGATTTAGTTTCCACCGCATCAGAGAGTATAGCTAGCTAG TTTTAAAGTTAGCATGATTTTTTCAAGATAACCCACCGTAGATTTTTTCAACATAATATAATATA ATTTTCACTTGTAAACTTTGAGGTTGCAAGGAAGAAAAGCAGGTAAAAAGAATAACAGGTAGCAA AGACATTTAAAAATTAAAATAGTTCTAACAATATAAGTCCAATCTAAAGGGGATACGTCCAGCAA TACTCATCCCTCACCAACTCCAACTTCACTCTCAATAAACTGGAATCGTGAAAGCATCATTACAA TTATCTCCTAGCTAACCAAACCCAACATTTTTTTTAGCTTTTAGAAATATTATCGCGTGCAATGT GATGCACTGCTGCAGTTAGCATCAACAAGAATAGTAACCTGACCCTTCATGCCATTATGATCGAG GTGGTAAAAAATGGCAAGTAGAAGTGAGGTTCATGCTCTTTAATGATTAATCTAATGGGATAACA AGAACCAGAACAAACAGAACTCTTGGTAGAAAAGAAAAAAAAAAGTGGGTCAATAATGCATATTT TGGATTCAAAACCACCACTGTCCAATTGACATCATTGTTCTACAAAACCGGAATGATTGTGATTC ATCCGGAGGGTATTTGCTCATTCATGTTCCTTATTATCGATATGGGCATACCTGACTAGCCAAGT ACAATTTCCTTAATTTCAATTTTGGCACTTACAATCGTGATTAAAACTGAGATCAGGTTTATATA TATGCTTGTCTTTTTATCCAAAAATTAGCATGCATTCTATATTTATGGGGTACGGGTCACGTGTG TACAATATACTCCTTACAAAAGGTTTATATATCTGCTTGGCTTTTAATCCCAAAATTAGCATGCA TTAAATGAAGGGTAACGTGTGTTTTATTCTTATTTAAATAAATAACATATAGTACAATTTTTAAG TAGCCAATAATTTTAAAATTTTCACTAACTCTGTATCTGTATTGTAATGAAAATATTTTTATATT TTACTTTTGGATCAATTTAAATTTATTTGTAAACAAATGGTTTTACATTTTATTAATTTCTTTTA TTAAATCTGTCCATAATATCTTTTTTTTTTATAAGTTTTAAATTTTATAATTTTAATTTAAATTT CTAATACAACGTAAGAGGATTAATATACTTAGCTAGTTAAAGATTATAATAATTATTTTCAACTG CGTTGGAGTTAGCTGGGATGACCACGGATCTTCCCCCCCATAAATTACCACAAAGCACCCCATTT GTTACACAGAAAGGGACTCTTGCAACAAGAGAATAAGGGACATTAAGTAATTTGCCTATTAATAA TGTTATAAGCTAATATAAAATTAGTTTGGCGGTTAAAATGAAAATTTAAAGATTGAAGGGAGAAA GAAGAAGAAAAGAGAGTTTTAAATTCAAATCTTCCACTGATCTTGGTTGATAAAAAAATGAAACC GCACACAAAAACGCTCTCCATCAATGCAATTGTACTAGTAATACTTAACTTGTGTCTTATATACA GCGTGGAAATATAAAATAAATAACATAATTATCATTTTTTGATAATATTATATATATATATATAT ATATATATAACTATTTTTTATATACGTTTGAGTACATAAGGAAACAATCTTGCTATTACCAATCT ATATTAGTTGTGGCTTTCCTCATAGAATTGATCCATGAAACGAAGGAGTAACACTGAATAATAAT AGTGCTAATGAAAAACCCATTATAATAGTAATTACTAATATTATTATGAAATATGAAATGTTAAT ATTCGGTGACTGCTTGGTCATTTTCTCTTCCAGAAAAACAGAGCTGTGACCTGTTAGTAAGGCCA TGGTGGGAGGGACCACTGCATGGCATCTTTCTCAGTGCTTCCCTTATTACATGATTTTGATGGCT TCAGTTGTCAGAGACCGGGTGGGTGGGTAGAAGATGGAGTATTGTATAGGAAGAAAATGGTAAAA TCATATTGAATCTTCTGCAATCCCCAATGTACTCTAGTTAGTAACTGTAATGTAAGGGCCTATTG TAATAATTGAAGCAGCACAGGGGCGAAGTCTCACATTCATAATAGGGTTTATCGAAAACACCACA CCATACCACTTGCCACGCCCCCTCTCTTTTCGTGACGGTCAACATTCTTTGACCACCTTTATCCA ACCTAACTAAATCATTACTACTGTTTATTAATTTATACTCTTGTTTTAATTTTCTATATTGAATT TCATTCATTTGTAATATTAATATAGGTGTGAAAATGACCATGATCAATAAAAAGAAAGGAAGAGC AATATCTAGCTTTTAGTGATAACATTGGACTTCTTTTTTGTTTTAACAAAAATTAAAGAAACAAC AGTCATTTTTCTGTCATAACTTGATGCCTTGACAAATTAATTTAATACTGTAAGATTATTGTAGA ACCCGTGATTATGCAGTAGAAGAACATAAATTTGTATGTTTCTCATCTAAGAAAGGAAAAGTAGC TAGAAAAAGAATGGTAGAGAGGGTGAAATAGCGAAATGCATGCTATGGCCAACGGGTTCCTTATT CCTTGCGGAGGCTATACAGTAGAATGGTTGTCCTAGATCACTTCAAATAGAATACGACACCCATG ATTGTCAAAAGGCTAAACAAGTTGATGCGCGCAATTTCCTTGTTTTCAGTCATAATTTTGGACTA AACTCCACACAGAACGACATGTTCTTTTCTAGGCTTTTGCTTTGTTGAATACTAGCGTTGGATTT TACTCTCTTCCTCACAAGATGGTAACAAGTTAGATAATCTATATAAGATTTCTGACCTTATTCGT CTTAATATAATAAACATGTTATAGCGAGTATATATCATGTGCTCCAATACATGCAGTTTTGGCAA TGGATTAGAAGTGTTAACGTTCCAGCAGAGTAATTCATACCATCCCCCATTTTTAATGCTCAAAA TGAGCAAGATGAAAATTTTTTAAACGTATCTTAATTCTTAGGGCATTCATTATTATCAAAAAGCC TTTATATTCATTAGAACTCTTTGCATGTATAGATCATTTTCTCTTTTTTTTTATTAAAAAAATTA ACATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA TATATATATCAGGCCATTCATTAAGCAATTAAACATGAAAATATTTTATACAAAATATTATTATA TCTATTGTAAGTTTTAATTAAAAAATTCATTGATATTCTTAAAACGTTTGTTAGTAAAAAATATA TTTTAAATTTAAAAGGTTATATATAGTTATACATTATTATATTATTCTTAAAATTTAAAACTTAA TAATAATAAAATAAAAAATAGTATTCTTAATAACTAGACACGACAACAAAATAAATAAATAATTA AGACAAGGAAAACTAACAGAAGAATTAGCCGTTTTCCTCGACCTTCCTTGGAAAATAAGGCAATA GCATAGGACCTACTTAAAAAAAGTTAAAACATTCGACTACAAAAACATACAAAATGGACAAAGAT AAACACGTAAGAAAAACTAAGAAAAACGTTACATTTTTTTCTTTTCAATTTCACGTATTGTTATT GAAAATTTTATTTCAAACATTGTTCATTTATTTGTTTTTTTAAGAGAGTTCATTCATTTGTTATT AATTTAACAAATTATTTGTTAACGATCTATTTTAAAATTCAAAACCTATTTTTATTAAACTCATT AAATTATGTGCACCATTTTTTTTATTATAAATATAATAATAACTGTTATATAAATTTGATGAATG ACATGATAAAAGACCGTATTATTTGCATAATTAAAGAAGCACGCCATATTTATGTCTTTCCTATC ACAATCATAAGTAAAACTTGAGTTTACCACCATCCTCCGCTCAATAACCCAGCAACACACATAAC ATTCTTTTATTAATGTCATTTTTAAGTGGCATAATAACTATATAACAACACACATGAGTGCCGCA TCATAAATTACACATACGATAAATAAATCTTCATTATTATCTTATGCAATTATATATGTATTATG AGTGGTTCATTAAAAAATAGTGCAGCAAAGTCACCATAGCCGTGGGTGAATGATTGATAGGTAAA ATTGTATTTTTCTTTTTTTCCCGGGTATTTCAAAAAGTAAAAAGAGTTGAAGGGACGAATTCATA TATTCAGAAAATTCCCTCTCCTTTAAGTATCGGTTTGTGTTTGGGGGCATCACTCGTTGTTTCTC TCTTCCATGCCCAAAATGCGTGTCCTCTTTGTTTTTCTGTTTTTCCATTTTCATTTCCCTGAAAC CCTTTCTGCCCCAATCTCAGAGTACCGCGCCCTTCTCTCTCTCCGTTCAGTCATTACCGACGCCA CACCACCCGTTCTCTCTTCTTGGAACGCCTCCATCCCTTACTGTTCCTGGCTCGGCGTCACCTGC GACAACCGCCGCCACGTCACCGCCCTCAACCTCACCGGCCTCGACCTCTCCGGCACGCTCTCTGC CGACGTCGCCCACCTCCCTTTCCTCTCCAACCTCTCCCTCGCCGCAAACAAATTCTCCGGCCCCA TTCCTCCCTCTCTCTCCGCCCTCTCCGGCCTCCGCTACCTCAACCTCTCCAACAATGTCTTCAAC GAAACCTTCCCCTCGGAGCTTTGGCGCCTCCAGAGCCTCGAGGTCCTCGACCTCTACAACAACAA CATGACCGGCGTGCTCCCTCTTGCCGTCGCGCAGATGCAGAATCTTCGTCATTTGCATCTCGGCG GCAACTTCTTCTCCGGCCAGATCCCGCCGGAGTACGGACGCTGGCAGCGCCTCCAGTACCTCGCC GTCTCCGGCAACGAACTCGACGGGACTATCCCGCCGGAGATCGGAAACTTGACCAGCCTCCGGGA GCTCTACATCGGCTACTACAACACCTACACCGGCGGCATTCCGCCGGAGATCGGAAACTTGTCGG AGCTGGTGAGGCTTGACGTAGCGTACTGTGCGTTGTCCGGGGAGATTCCGGCGGCGCTTGGGAAG CTTCAGAAGCTGGACACGCTGTTCCTTCAGGTGAATGCATTGTCAGGATCACTGACGCCGGAGCT GGGGAACCTGAAGAGCCTGAAATCCATGGATTTGTCTAACAACATGCTCTCCGGTGAGATTCCGG CGAGTTTCGGCGAGCTGAAGAATATTACGCTTCTGAATCTGTTCAGGAACAAGCTTCATGGAGCT ATACCGGAGTTTATAGGAGAGCTTCCAGCGTTGGAAGTTGTGCAACTGTGGGAAAATAACTTAAC AGGTAGCATTCCTGAGGGTTTGGGCAAAAATGGGAGACTCAACCTTGTTGATCTTTCTTCTAACA AGTTAACCGGGACTTTGCCTCCTTATCTCTGTTCTGGGAATACTCTTCAGACTCTGATAACTCTT GGGAATTTTCTTTTCGGTCCAATTCCTGAGTCGCTCGGGACTTGTGAATCTCTTACACGGATTAG AATGGGAGAAAACTTTTTGAATGGTTCCATTCCTAAAGGGCTTTTTGGACTTCCCAAACTCACCC AGGTTGAACTTCAGGATAATTATCTCTCTGGAGAGTTTCCTGAGGTTGGTTCTGTTGCGGTTAAT CTTGGTCAGATTACTCTCTCTAACAACCAGCTTTCTGGGGCTCTGTCTCCCTCCATTGGTAACTT CTCCAGCGTGCAGAAGCTCCTTCTTGATGGCAACATGTTCACCGGTCGGATACCTACACAGATTG GGAGGTTGCAACAGCTTTCTAAGATTGATTTTAGTGGCAACAAGTTCTCGGGTCCTATTGCGCCT GAGATCAGTCAGTGTAAGCTGTTAACTTTCCTGGACCTTAGCCGCAATGAGCTATCTGGAGACAT CCCTAATGAGATAACTGGCATGAGGATATTGAATTACTTGAATCTTTCTAAGAATCATTTAGTGG GTAGCATTCCCTCTTCGATATCATCTATGCAAAGCTTGACTTCTGTTGATTTTTCATACAACAAC CTGTCTGGTTTGGTGCCTGGTACCGGTCAATTCAGCTACTTCAACTACACGTCTTTCTTGGGAAA CCCTGACCTGTGTGGCCCCTATTTGGGTGCTTGCAAGGGTGGGGTTGCCAATGGTGCACACCAAC CTCATGTTAAAGGACTCTCCTCTTCTTTGAAGCTGCTACTTGTTGTTGGGTTGCTATTATGTTCC ATTGCTTTTGCTGTGGCTGCAATATTCAAGGCCCGGTCATTAAAGAAGGCCAGTGAGGCTCGTGC ATGGAAGTTGACTGCGTTCCAGCGTTTGGACTTCACTGTTGATGATGTTTTGCATTGCTTGAAAG AGGATAATATTATTGGGAAAGGAGGTGCTGGAATTGTCTACAAAGGGGCTATGCCTAATGGGGAT CATGTTGCTGTGAAAAGGCTTCCAGCTATGAGTAGAGGCTCTTCCCATGATCACGGATTCAATGC TGAGATTCAGACATTGGGGCGAATCCGACACAGGCACATTGTTAGGTTGTTGGGTTTCTGTTCAA ATCATGAGACAAACCTTTTGGTCTATGAGTACATGCCCAATGGAAGTTTAGGTGAGGTTCTTCAT GGAAAAAAGGGGGGTCATTTGCATTGGGACACCAGGTATAAAATTGCGGTGGAGGCTGCCAAGGG GCTTTGCTATCTGCACCATGATTGTTCGCCACTCATTGTCCATCGTGATGTGAAGTCAAACAACA TCCTTCTTGATTCAAATCATGAAGCCCATGTTGCTGATTTTGGGCTTGCTAAGTTCCTGCAAGAT TCTGGGACATCTGAATGCATGTCTGCTATTGCTGGTTCATATGGATACATAGCTCCAGGTACCGT TGAATTTTGACATAATTAATGCATCATATGCATGGTTGTGGCAAATTTCCTTTTTCTCGCCTAAT CATAATTGTACGTTTAAGCATTTTGTTCAGAATTTGACTCTTTGACTTATGCATGATATTGAGGT GATGCCCCTAAATTTATTAACATTGCTATGTGGTTTTTCTTGACTTTGGTTTTCTATCATACCCA ATTGATTCGCCCCCTTATTTTGTTTTTTTTTCTAAGCCAAGTACTGAAAGTAAATGGTAGGTATC TCTGCACCGTTTGATTTTTTACCCTAACCCCCTCTCCCCACCTATGAAGTAGATAATGCTGTAGT CGTAGGTTAAGAGTCATTCACAATCGGAAACTGATGGTTATGGGCAAAAACATCAGATAAAAAGA CCTATTATGTTACTTTATACGTATTGCCTTTGTTTAACTTATTGTTTCAAATTAAAGTGTCTTGC TTTATTATAGTGTATGATACCTGTTGGATGTTTGATTGCAGAGTATGCCTACACATTGAAAGTTG ATGAGAAAAGCGATGTGTACAGTTTTGGTGTGGTTCTTTTAGAACTTATAACAGGCAGGAAACCA GTTGGTGAATTTGGTGATGGCGTGGACATCGTGCAATGGGTGAGGAAAATGACGGACTCTAACAA GGAAGGAGTTCTTAAAGTTCTTGATCCTAGGCTTCCCTCAGTTCCCCTTCACGAAGTGATGCATG TTTTCTATGTGGCCATGCTGTGTGTTGAAGAACAGGCTGTAGAGAGACCAACAATGCGTGAAGTT GTTCAAATACTGACCGAGCTTCCAAAGCCACCTGGCTCTAAAGAGGGAGACTTAACAATAACAGA ATCCTCTTTGTCATCATCAAACGCTTTAGAATCTCCATCCTCAGCCTCCAAGGAAGATCAAAATC CTCCTCAATCCCCACCACCCGACCTTCTTAGTATTTAAAGTGCTCTGTTGGGTGTTTCATCTTAT TAGTTCCCTTGGTTGTGATAGCTTATCCATTTACTTTCTTTTTCTGTCTCTCTTCTGGGGTTGGG GCTTTTCTTCTTCTTCTAACTGAAGGTATTAATGCTCTGATTTTTTAATGGTTTTGTACAGTAGG ATTGGTGGGGGGGGTTATTTTCTTATGAAGTCACTTTCTTCATCATGTAGTACTGCTTTTTAATT TTTATGTTACGGCCGTTGTTGTGCTTCGCCTAAGCTGGGGAGTGGGGAGGGTTCAAGGGAATGGA TACTCTTTTTTTATGCGATCACTGACAGGTAGACACAAAATGACGCAAACGGGTTGGGTATTAAA CAGTGGGTATATTGTATGGTTTAGAATATTATTGATGAATCCTGAGTGGATTGGCACAGTGTGAA CTGTGAGCCTGAGCTGTGACTGAGTCTATGAGTCAGGTTTGGATAAAAGCTTATTTGAAGAAGTT AACCTGTTTCGAGAAAATCAGAGTGAATCAGGATTCAGGCGTGTTTTAGCTTT Glyma11g04700 ATGCCCAAAATGCGTGTCCTCTTTGTTTTTCTGTTTTTCCATTTTCATTTCCCTGAAACCCTTTC cDNA (SEQ ID TGCCCCAATCTCAGAGTACCGCGCCCTTCTCTCTCTCCGTTCAGTCATTACCGACGCCACACCAC NO: 33) Soybean CCGTTCTCTCTTCTTGGAACGCCTCCATCCCTTACTGTTCCTGGCTCGGCGTCACCTGCGACAAC BAM2-like gene CGCCGCCACGTCACCGCCCTCAACCTCACCGGCCTCGACCTCTCCGGCACGCTCTCTGCCGACGT CGCCCACCTCCCTTTCCTCTCCAACCTCTCCCTCGCCGCAAACAAATTCTCCGGCCCCATTCCTC CCTCTCTCTCCGCCCTCTCCGGCCTCCGCTACCTCAACCTCTCCAACAATGTCTTCAACGAAACC TTCCCCTCGGAGCTTTGGCGCCTCCAGAGCCTCGAGGTCCTCGACCTCTACAACAACAACATGAC CGGCGTGCTCCCTCTTGCCGTCGCGCAGATGCAGAATCTTCGTCATTTGCATCTCGGCGGCAACT TCTTCTCCGGCCAGATCCCGCCGGAGTACGGACGCTGGCAGCGCCTCCAGTACCTCGCCGTCTCC GGCAACGAACTCGACGGGACTATCCCGCCGGAGATCGGAAACTTGACCAGCCTCCGGGAGCTCTA CATCGGCTACTACAACACCTACACCGGCGGCATTCCGCCGGAGATCGGAAACTTGTCGGAGCTGG TGAGGCTTGACGTAGCGTACTGTGCGTTGTCCGGGGAGATTCCGGCGGCGCTTGGGAAGCTTCAG AAGCTGGACACGCTGTTCCTTCAGGTGAATGCATTGTCAGGATCACTGACGCCGGAGCTGGGGAA CCTGAAGAGCCTGAAATCCATGGATTTGTCTAACAACATGCTCTCCGGTGAGATTCCGGCGAGTT TCGGCGAGCTGAAGAATATTACGCTTCTGAATCTGTTCAGGAACAAGCTTCATGGAGCTATACCG GAGTTTATAGGAGAGCTTCCAGCGTTGGAAGTTGTGCAACTGTGGGAAAATAACTTAACAGGTAG CATTCCTGAGGGTTTGGGCAAAAATGGGAGACTCAACCTTGTTGATCTTTCTTCTAACAAGTTAA CCGGGACTTTGCCTCCTTATCTCTGTTCTGGGAATACTCTTCAGACTCTGATAACTCTTGGGAAT TTTCTTTTCGGTCCAATTCCTGAGTCGCTCGGGACTTGTGAATCTCTTACACGGATTAGAATGGG AGAAAACTTTTTGAATGGTTCCATTCCTAAAGGGCTTTTTGGACTTCCCAAACTCACCCAGGTTG AACTTCAGGATAATTATCTCTCTGGAGAGTTTCCTGAGGTTGGTTCTGTTGCGGTTAATCTTGGT CAGATTACTCTCTCTAACAACCAGCTTTCTGGGGCTCTGTCTCCCTCCATTGGTAACTTCTCCAG CGTGCAGAAGCTCCTTCTTGATGGCAACATGTTCACCGGTCGGATACCTACACAGATTGGGAGGT TGCAACAGCTTTCTAAGATTGATTTTAGTGGCAACAAGTTCTCGGGTCCTATTGCGCCTGAGATC AGTCAGTGTAAGCTGTTAACTTTCCTGGACCTTAGCCGCAATGAGCTATCTGGAGACATCCCTAA TGAGATAACTGGCATGAGGATATTGAATTACTTGAATCTTTCTAAGAATCATTTAGTGGGTAGCA TTCCCTCTTCGATATCATCTATGCAAAGCTTGACTTCTGTTGATTTTTCATACAACAACCTGTCT GGTTTGGTGCCTGGTACCGGTCAATTCAGCTACTTCAACTACACGTCTTTCTTGGGAAACCCTGA CCTGTGTGGCCCCTATTTGGGTGCTTGCAAGGGTGGGGTTGCCAATGGTGCACACCAACCTCATG TTAAAGGACTCTCCTCTTCTTTGAAGCTGCTACTTGTTGTTGGGTTGCTATTATGTTCCATTGCT TTTGCTGTGGCTGCAATATTCAAGGCCCGGTCATTAAAGAAGGCCAGTGAGGCTCGTGCATGGAA GTTGACTGCGTTCCAGCGTTTGGACTTCACTGTTGATGATGTTTTGCATTGCTTGAAAGAGGATA ATATTATTGGGAAAGGAGGTGCTGGAATTGTCTACAAAGGGGCTATGCCTAATGGGGATCATGTT GCTGTGAAAAGGCTTCCAGCTATGAGTAGAGGCTCTTCCCATGATCACGGATTCAATGCTGAGAT TCAGACATTGGGGCGAATCCGACACAGGCACATTGTTAGGTTGTTGGGTTTCTGTTCAAATCATG AGACAAACCTTTTGGTCTATGAGTACATGCCCAATGGAAGTTTAGGTGAGGTTCTTCATGGAAAA AAGGGGGGTCATTTGCATTGGGACACCAGGTATAAAATTGCGGTGGAGGCTGCCAAGGGGCTTTG CTATCTGCACCATGATTGTTCGCCACTCATTGTCCATCGTGATGTGAAGTCAAACAACATCCTTC TTGATTCAAATCATGAAGCCCATGTTGCTGATTTTGGGCTTGCTAAGTTCCTGCAAGATTCTGGG ACATCTGAATGCATGTCTGCTATTGCTGGTTCATATGGATACATAGCTCCAGAGTATGCCTACAC ATTGAAAGTTGATGAGAAAAGCGATGTGTACAGTTTTGGTGTGGTTCTTTTAGAACTTATAACAG GCAGGAAACCAGTTGGTGAATTTGGTGATGGCGTGGACATCGTGCAATGGGTGAGGAAAATGACG GACTCTAACAAGGAAGGAGTTCTTAAAGTTCTTGATCCTAGGCTTCCCTCAGTTCCCCTTCACGA AGTGATGCATGTTTTCTATGTGGCCATGCTGTGTGTTGAAGAACAGGCTGTAGAGAGACCAACAA TGCGTGAAGTTGTTCAAATACTGACCGAGCTTCCAAAGCCACCTGGCTCTAAAGAGGGAGACTTA ACAATAACAGAATCCTCTTTGTCATCATCAAACGCTTTAGAATCTCCATCCTCAGCCTCCAAGGA AGATCAAAATCCTCCTCAATCCCCACCACCCGACCTTCTTAGTATTTAA Glyma11g04700 MPKMRVLFVFLFFHFHFPETLSAPISEYRALLSLRSVITDATPPVLSSWNASIPYCSWLGVTCDN protein (SEQ RRHVTALNLTGLDLSGTLSADVAHLPFLSNLSLAANKFSGPIPPSLSALSGLRYLNLSNNVFNET ID FPSELWRLQSLEVLDLYNNNMTGVLPLAVAQMQNLRHLHLGGNFFSGQIPPEYGRWQRLQYLAVS NO: 34) Soybean GNELDGTIPPEIGNLTSLRELYIGYYNTYTGGIPPEIGNLSELVRLDVAYCALSGEIPAALGKLQ BAM2-like gene KLDTLFLQVNALSGSLTPELGNLKSLKSMDLSNNMLSGEIPASFGELKNITLLNLFRNKLHGAIP EFIGELPALEVVQLWENNLTGSIPEGLGKNGRLNLVDLSSNKLTGTLPPYLCSGNTLQTLITLGN FLFGPIPESLGTCESLTRIRMGENFLNGSIPKGLFGLPKLTQVELQDNYLSGEFPEVGSVAVNLG QITLSNNQLSGALSPSIGNFSSVQKLLLDGNMFTGRIPTQIGRLQQLSKIDFSGNKFSGPTAPEI SQCKLLTFLDLSRNELSGDIPNEITGMRILNYLNLSKNHLVGSIPSSISSMQSLTSVDFSYNNLS GLVPGTGQFSYFNYTSFLGNPDLCGPYLGACKGGVANGAHQPHVKGLSSSLKLLLVVGLLLCSIA FAVAAIFKARSLKKASEARAWKLTAFQRLDFTVDDVLHCLKEDNIIGKGGAGIVYKGAMPNGDHV AVKRLPAMSRGSSHDHGFNAEIQTLGRIRHRHIVRLLGFCSNHETNLLVYEYMPNGSLGEVLHGK KGGHLHWDTRYKIAVEAAKGLCYLHHDCSPLIVHRDVKSNNILLDSNHEAHVADFGLAKFLQDSG TSECMSATAGSYGYIAPEYAYTLKVDEKSDVYSFGVVLLELITGRKPVGEFGDGVDIVQWVRKMT DSNKEGVLKVLDPRLPSVPLHEVMHVFYVAMLCVEEQAVERPTMREVVQILTELPKPPGSKEGDL TITESSLSSSNALESPSSASKEDQNPPQSPPPDLLSI Glyma09g38720 CACGTGGTACACGAACACCGACGCCATCAGAATCCAAAAGGGTATCAGGAATCACAATCAAAAAC gDNA + about GAATTTTGTTCTAGTTTTTATATCCTTAAAAAATTCGAAACCAGAGAGAGAAAAAAAATGGTTGG 1 kb of GTTTTTTTACTCTTGTCGGGTGAGAGCTATAAGAGGGTGTGGAGGAAGATGAGGAGAAGATCGAG promoter and GGCGGTGATGGGATGGCGGTGGAGGATCACAGCAGAGAAATAGTTTGCCATTGCCATGGAGGGAG 5′UT sequence AGCGAAGAGGTTGAGGCCCATTCAATTGAATTGGATCAGAGAGAGTTAACTGAAGAATCGGTCAC (SEQ ID TGAGAAAAGGGCGCGTAGCTTAGCATTTGATATGTGGCGATTTGGTTTGGGTACGTCCTTTCGGG NO: 35) Soybean GACAGAAGAAGATGGATCAAAGACGCTTAATGCGGTTGGGACCTGAGAATGAATGAGAGAGACAC CLV2-1ike gene TCACTACACTCACAAAAGGAGGTTCAATTTATCAAATAAAAAAGAGAGACACAGGGGATGGATGT GTCATGTGTGTGTCCATGTGTGGTGAGCTCCATCATATAGAGAATCTTTTCACCTTAATTATTTT TTAAGGCTATTCTTAATCAGTAATCTTAGACATTGATTAAAAAATTAAAAAGAAAATATAAAATA AGTTGTAGAGCACTATAATTTAATATTTTAATATAAAAAGTATTTAGAAGAATGATAAATATATC TAGCTTTCTTAATATATAAAATTAATATAAATTAGTATAATATCACAAATATTTTATTAAACCAA ACAATTAACATTTTAAAAATTTTATATTTGATTTTTACTGTGTCTAAAATTTTTTGGGTCGCTGA TAACCACAAATTACAAACAAAATTAATCTCCCATTGAATTAAAAAATAACATAATCTATAACCTA TCAAAAAGAAAAAGAAAAAAGAATCTGGACCTATTTCTACCCCGATGCACATGAGAAACTTAAAA AGGGGGTGAAGTGTTATGTAGTATAGAGAGAAAGCGAGGGAAGGCAAAGCAAGCACAACAGAACA AAGCCACTTTATTTTTTTGATCTAACCTAAACCATCCTTTCCCCCTGTTGCACTCTCACTTTATC AACGTGACACAAGCAACTTATGACCAATGTGTAAGATGTTGTTCCTCTTTCCCTTCTCTTCTGTC CATTTCATCAAGTTTCCATTCTAATCTCCAAATCTTTGCCACCCCAGTTCCTCTTTTGCTTCAAA CTTCTCTTCCCCTCCCTAAAAATTGCACCTTTACTCTCATGGTGATGGGACACACCACACCCCTC ACACTCCTCTGTATGATTCTTCTTTTTGCAACCCCTTCTCTCTCAATTGATGTTCACCCACAAGA CAGAATCTCACTCTCACTGTTCAGGTCATCTCTGCCAAACCCCAACCAGAGTTTGCCCAGCTGGG TAGGCTCCAACTGCACTTCATGGAGTGGAATCACCTGCGACAGCAGAACTGGGAGAGTGCTTTCC ATCAACCTAACTAGCATGAACCTTTCAGGCAAAATCCACCCCAGTTTGTGCCACCTTTCATACCT CAACAAGTTGGGGTTGTCACACAACAACTTCACAGCCCCACTTCCTGAGTGTTTTGGAAACTTGC TTAACCTAAGAGCCATTGATCTCAGCCACAACAGGTTTCATGGTGGAATACCAGACTCTTTCATG AGGCTCAGGCACCTCACTGAGCTTGTTTTCAGTGGGAACCCTGGTTTGGGGGGTCCACTTCCTGC TTGGATTGGTAACTTCTCTGCAAATCTGGAAAAGTTACATCTTGGTTTCTGTTCATTCAGTGGTG GCATACCTGAGAGCTTGCTTTACATGAAGTCCCTCAAGTATTTGGACCTTGAGAACAATCTCTTG TTTGGTAATTTGGTTGATTTTCAACAGCCTTTGGTTTTGCTCAATCTTGCTTCCAATCAGTTTGC TGGTACTTTGCCTTGCTTTGCAGCTTCAGTTCAGTCTCTAACTGTGTTGAATTTGTCCAACAATT CTATTGCGGGGGGATTGCCTGCTTGTATTGCTTCTTTTCAAGCTTTGACTCATTTGAACCTTTCA GGGAACCATTTGAAGTATAGAATATATCCTAGGCTTGTGTTCTCAGAGAAACTTCTTGTTTTGGA CTTGAGTAATAATGCTTTATCTGGTCCTATTCCCAGTAAAATTGCTGAGACTACTGACAAACTTG GCCTTGTTCTTCTTGACCTTTCTCACAATCAGTTCTCTGGTGAAATACCTGTGAAAATTACTGAG TTGAAAAGCTTGCAGGCCTTGTTTCTCTCTCACAATCTTCTCTCAGGAGAAATTCCTGCTAGAAT TGGAAATTTGACTTATCTGCAGGTCATTGATCTCTCACACAACTCTTTGTCTGGAACCATTCCAT TCAGTATTGTTGGGTGCTTTCAGCTGTATGCTCTGATACTTAACAACAACAATCTTTCTGGTGTA ATTCAACCGGAGTTTGATGCGTTGGATATCTTGAGGATACTGGATATAAGCAACAACAGGTTTTC CGGGGCTATCCCACTCACTTTGGCTGGATGCAAATCTTTGGAGATTGTAGACTTTAGTTCCAATG AGCTTTCTGGATCGTTGAATGATGCAATAACCAAATGGACAAACCTCAGGTATTTGTCTCTTGCT CAGAACAAGTTCAGTGAAAATCTGCCTAGTTGGTTGTTCACATTTAACGCAATAGAAATGATGGA TTTCTCGCATAACAAGTTTACTGGCTTCATACCGGATATTAATTTTAAGGGTAGCTTAATATTTA ACACTAGGAATGTCACTGTTAAAGAGCCATTGGTTGCAGCAAGAAAGGTTCAACTCAGAGTTTCG GCGGTTGTTTCTGATAGCAATCAACTCAGTTTCACTTATGATCTTTCCTCAATGGTTGGAATTGA TCTATCCAGCAACTCGCTTCATGGGGAAATTCCAAGGGGCTTATTTGGTCTATCTGGCCTAGAAT ATCTGAATTTGTCATGCAACTTTCTTTACGGACAGCTTCCGGGGTTGCAGAAAATGCAGAGTTTG AAAGCCTTGGATTTGTCACATAATTCCTTGTCAGGACATATCCCAGGAAACATCTCTATCCTTCA AGATCTGTCTATTTTGAATCTTTCCTACAACTGCTTTTCTGGATGTGTTCCCCAGAAGCAAGGGT ATGGGAGATTTCCTGGTGCATTTGCTGGAAATCCAGATCTGTGCATGGAATCTTCCAGTGGATTA TGTGATGATGGAAGGACTCAATCTGCGCAAGGAAGTACTTTTAGGGAAGATAGGATGGATGACCC AATTTCTGTGGGGATTTTCTTTATCAGTGCATTTGTTAGTTTTGATTTTGGTGTTGTGGTTCTCT TCTGTTCCGCACGGGCAAGAAATTACATTCTCCAAACAAAAGTTTGATTTGATGCTTGTGACACA TACAAATCTCCTGTAAATTCCATTTTGTAATGTGGTACCTGTCTTCTCAGTTTCAAGTAAACATA CACTTACGTGACTGGGAATACTATCTGGCCATCAGCTTCACAAGTGTTTTCTCGTGATTACTGAA CAAGTGTCTCGGAATTGCAGGATCAAAATGCCATGATATGAGTAACACAAGGTTTAAAGAACACT CATAACGCTGGCTTTAACTATCTGAGTGAAGACTAGTCCTGCATCATTCAGCCAAGAAAAAAATG GATGGTTATGATGAAAATTTGATCCAAGTAAAGACGAGTCCCTCATCATTCTGATGGTTGTTCTC TTTTGCTGGAACTTGGTTGCATCAAGTTTATTATGCATCATCACATGCATTATTCATAATCAGGT GGGTGAAGGGTCAGCAAGGAACATGCCTGATTGATATCTGGTCTAGTTATGGTGAAATTTTGATC TTGGGACATCAAATTGCAGATTTGCAAGCATGTTTACGTGAAGAGAACTTGTATCATTCTAGATT AACCCAGCTCTTTCTTGAGGTGGGGAACCAAGTTTTCCCTGTAAGTGTTTTACCTTAAGAATGTG AGTTGATGAGTAGTGGGGAGTGGTAAGTGCAGACAAAATAAATGGAGTAGTTCTCATAAATCTAA GATTTGTATTTGTATTACTGTCTTCATGCCTTCATCTTAGTGCTGTGATTTTAAATGAAATTCTC ACGAAATCTTTTCATTGAGAACAGAAAAGAGGTAATTGAGCACCTTAGCTTTGTTATCAAATGCC AAGCATGCTCAACAAAAATTAGAAAAATTATCTAGTTTACCAA Glyma09g38720 ATGGTGATGGGACACACCACACCCCTCACACTCCTCTGTATGATTCTTCTTTTTGCAACCCCTTC cDNA (SEQ ID TCTCTCAATTGATGTTCACCCACAAGACAGAATCTCACTCTCACTGTTCAGGTCATCTCTGCCAA NO: 36) Soybean ACCCCAACCAGAGTTTGCCCAGCTGGGTAGGCTCCAACTGCACTTCATGGAGTGGAATCACCTGC CLV2-like gene GACAGCAGAACTGGGAGAGTGCTTTCCATCAACCTAACTAGCATGAACCTTTCAGGCAAAATCCA CCCCAGTTTGTGCCACCTTTCATACCTCAACAAGTTGGGGTTGTCACACAACAACTTCACAGCCC CACTTCCTGAGTGTTTTGGAAACTTGCTTAACCTAAGAGCCATTGATCTCAGCCACAACAGGTTT CATGGTGGAATACCAGACTCTTTCATGAGGCTCAGGCACCTCACTGAGCTTGTTTTCAGTGGGAA CCCTGGTTTGGGGGGTCCACTTCCTGCTTGGATTGGTAACTTCTCTGCAAATCTGGAAAAGTTAC ATCTTGGTTTCTGTTCATTCAGTGGTGGCATACCTGAGAGCTTGCTTTACATGAAGTCCCTCAAG TATTTGGACCTTGAGAACAATCTCTTGTTTGGTAATTTGGTTGATTTTCAACAGCCTTTGGTTTT GCTCAATCTTGCTTCCAATCAGTTTGCTGGTACTTTGCCTTGCTTTGCAGCTTCAGTTCAGTCTC TAACTGTGTTGAATTTGTCCAACAATTCTATTGCGGGGGGATTGCCTGCTTGTATTGCTTCTTTT CAAGCTTTGACTCATTTGAACCTTTCAGGGAACCATTTGAAGTATAGAATATATCCTAGGCTTGT GTTCTCAGAGAAACTTCTTGTTTTGGACTTGAGTAATAATGCTTTATCTGGTCCTATTCCCAGTA AAATTGCTGAGACTACTGACAAACTTGGCCTTGTTCTTCTTGACCTTTCTCACAATCAGTTCTCT GGTGAAATACCTGTGAAAATTACTGAGTTGAAAAGCTTGCAGGCCTTGTTTCTCTCTCACAATCT TCTCTCAGGAGAAATTCCTGCTAGAATTGGAAATTTGACTTATCTGCAGGTCATTGATCTCTCAC ACAACTCTTTGTCTGGAACCATTCCATTCAGTATTGTTGGGTGCTTTCAGCTGTATGCTCTGATA CTTAACAACAACAATCTTTCTGGTGTAATTCAACCGGAGTTTGATGCGTTGGATATCTTGAGGAT ACTGGATATAAGCAACAACAGGTTTTCCGGGGCTATCCCACTCACTTTGGCTGGATGCAAATCTT TGGAGATTGTAGACTTTAGTTCCAATGAGCTTTCTGGATCGTTGAATGATGCAATAACCAAATGG ACAAACCTCAGGTATTTGTCTCTTGCTCAGAACAAGTTCAGTGAAAATCTGCCTAGTTGGTTGTT CACATTTAACGCAATAGAAATGATGGATTTCTCGCATAACAAGTTTACTGGCTTCATACCGGATA TTAATTTTAAGGGTAGCTTAATATTTAACACTAGGAATGTCACTGTTAAAGAGCCATTGGTTGCA GCAAGAAAGGTTCAACTCAGAGTTTCGGCGGTTGTTTCTGATAGCAATCAACTCAGTTTCACTTA TGATCTTTCCTCAATGGTTGGAATTGATCTATCCAGCAACTCGCTTCATGGGGAAATTCCAAGGG GCTTATTTGGTCTATCTGGCCTAGAATATCTGAATTTGTCATGCAACTTTCTTTACGGACAGCTT CCGGGGTTGCAGAAAATGCAGAGTTTGAAAGCCTTGGATTTGTCACATAATTCCTTGTCAGGACA TATCCCAGGAAACATCTCTATCCTTCAAGATCTGTCTATTTTGAATCTTTCCTACAACTGCTTTT CTGGATGTGTTCCCCAGAAGCAAGGGTATGGGAGATTTCCTGGTGCATTTGCTGGAAATCCAGAT CTGTGCATGGAATCTTCCAGTGGATTATGTGATGATGGAAGGACTCAATCTGCGCAAGGAAGTAC TTTTAGGGAAGATAGGATGGATGACCCAATTTCTGTGGGGATTTTCTTTATCAGTGCATTTGTTA GTTTTGATTTTGGTGTTGTGGTTCTCTTCTGTTCCGCACGGGCAAGAAATTACATTCTCCAAACA AAAGTTTGA Glyma09g38720 MVMGHTTPLTLLCMILLFATPSLSIDVHPQDRISLSLFRSSLPNPNQSLPSWVGSNCTSWSGITC protein (SEQ DSRTGRVLSINLTSMNLSGKIHPSLCHLSYLNKLGLSHNNFTAPLPECFGNLLNLRAIDLSHNRF ID HGGIPDSFMRLRHLTELVFSGNPGLGGPLPAWIGNFSANLEKLHLGFCSFSGGIPESLLYMKSLK NO: 37) Soybean YLDLENNLLFGNLVDFQQPLVLLNLASNQFAGTLPCFAASVQSLTVLNLSNNSIAGGLPACIASF CLV2-like gene QALTHLNLSGNHLKYRIYPRLVFSEKLLVLDLSNNALSGPIPSKIAETTDKLGLVLLDLSHNQFS GEIPVKITELKSLQALFLSHNLLSGEIPARIGNLTYLQVIDLSHNSLSGTIPFSIVGCFQLYALI LNNNNLSGVIQPEFDALDILRILDISNNRFSGAIPLTLAGCKSLEIVDFSSNELSGSLNDAITKW TNLRYLSLAQNKFSENLPSWLFTFNAIEMMDFSHNKFTGFIPDINFKGSLIFNTRNVTVKEPLVA ARKVQLRVSAVVSDSNQLSFTYDLSSMVGIDLSSNSLHGEIPRGLFGLSGLEYLNLSCNFLYGQL PGLQKMQSLKALDLSHNSLSGHIPGNISILQDLSILNLSYNCFSGCVPQKQGYGRFPGAFAGNPD LCMESSSGLCDDGRTQSAQGSTFREDRMDDPISVGIFFISAFVSFDFGVVVLFCSARARNYILQT KV GmNARK: GCACCCACTGGGTAAGTTGGTAACTACTATGTATCTATATATCGTCAGGTCATTGTCTGTTTCAT Glyma12g04390 TCTCTTCTCACAAGAACAAAATGGTAATTTACATTTAACTTAGAAATGTTTGGGACAGAACCTCT gDNA + 5 kb AGCTTGCGATGATTCTCTTCTCACAAGAACAAAATGGTAATTTACATTTAACTTTAGAAATGTTT promoter and GGGACCGAACCTCTAGCTTGCGATGATTCTCTTCTCACAAGAACAAAATGGTAATTTACATTTAA 5′UT (SEQ ID CTTTAGAAATGTTTGGGACCGAACCACTAGCTTGCGATGATTCCCTTCTCACAAGAACAAAATGG NO: 38) Soybean TAATTTACATTTAACTTAGAAATGTTTGGGACAGAACCACTGGCTTGCGATGATTCTCTTCTCAC CLV1-like gene AAGAACAAAATGGTAATTTGCATTTAACTTAGAAATGATTGGGACAGAACCACTAGCTTCGATGA ATAATTTGCTTTAATTTTTATTAATGCATAATACCCTTTTATTGTCACACATAGAATCCGATTCT GCAATAACTAGTGCTTGATCCTAATTGACAGAACAAATTAAAACAGAGAATTGATGCTTTGGCTT TTCCATGGGCAATAATTATCCCAATGATATACTAAAGCATAGTAACTAGGAAGACTTCCATGTAA AGAAACTTTCTTTTATTCTCCTTTTAAAATTTGGTGAATCACTTAAAACCACTTTTGTTTCATTC CAAGGTTAGGCTCATGGAAAGCTTAAACCTACTTAACTGGTCACGAAGAGATTGCATCTTTGTTT TCACAAAAGTCTAACTCCAAGTTCGTGTAGCTAGTATTGCATGCTACCATGGTGCAAGTGATGTA CATGCATATATGATATTCAATTTAATTTGCTACTATAATATAAAGGTGTATATATAAATAGAGAG TGCATGAGGTGTGTGGTGTCAACATATAAGGACGCAGCAAAGGTATAATAGCGACTACTGCGAAG CAAGATCAGAGACTAGAGAGACATGATAAGAAGTTGTTAATTTGTTTTCTTCATATGGCTGCGCG TGGCAACGTGCTCTTCGTTCACTGACATGGATGCGCTGCTGAAGCTGAAGGACTCCATGACTGGA AGTTTTCCACGTCGCTTTCTGCACACTGTTTCTTTTCAGGCGTAAACTGCGACCAAGAACTTCGA GTCGTTGCTATCAATGTCTCGTTTGTTCCTCTTTTCGGCTACCTTCCGCCGGAGATCGGACAATT GGACAAACTCGAGAACCTCACTTCCCTCAAGCTCCTCGACATCTCTCACAACGTCTTCTCCGGCC AAATTATTCTTCCGATGACGAAACTGGAGGTCCTCGACGTCTACGACAACAACTTCCGGCAGCAT ACCGGAGATTTACTCGGAGTTTAAGAGCTTGGAGTTTTTAAGCTTAAGCACCAATAACTTATCGG GGAAGATTCCGAAGAGTTTGTCTAAGTTGAAGACGCTGAGGTATCTCAAACTCGGATACAACAAC GCTTACGAAGGTGAAATTCCACCGGAGTTTGGCAGCATAAAATCTGAGATACCTTGACCTCATCG GCGAGATTCCACCTACTCTAAACAATAATAAGAAAAACTTATCACATTTCTTGAAACTTTAAAAG ACCGATAAAAATAAAAGGAGGAAATGCCACTACAATATTTTTAATTTATTTTTTTTACTTATTTT ATTTGAATCTTTAATACATATGCTATTTTAGCATTATAAAAATACCTGGGCTATACAAAATATAC TTGCTAGTAGTATTATGTGTGTGTGAAAGTTAAATGAGTCTTTAAGTATTTGTAAATGTTTAATA AGTTTCGAGGTTTATCTTGATTCCAACAATGAATTCCTGAAATCTAATTTATCTAACTTTTTTTT AACCAAAATGTTAAATGGTCTAGTTAAGAGAACAAATCCTTATGTGTTCATTTTTTCACAAGACC TAAAATCTAAAATTTCACTTTAAAAGAAACAAAATACTTGCTACTTGAACTAACAATCATTAGTA CATTTTTTTAGTAATGATATACAAACATCTAAAACTCCTATACAACACAACACATAGAAGACAAT AAAAAATATCAATATGATAAATAAAAATGAGAAATAGATGAATTATTTAAAATAATGAAATGTTT ATTTATCATTACTTTTTTTTACTTTAACAGTTCATACATCTCCTACAAGGTAAGATGTGTAATGC AAGTAAGTTGCAACATGGTTTTAAATTTTGACAATAAGAACCATGCATGTTAATTAGTCTAATCA CAGAGCGTTCGGGATACGCCATTAGTGGTCTATAGTAGTCAACTGCCGGGATAAATCACGATCCA CATTTCATAGGTGTTTCCACCATGTCAACATCGAACTAAAAGGAAAAATATGTGAATGGGTAAAA ATGATTAAAAATATTTGTAAAAAATTATTTGAATTTATTTAAAACAATATGCAAGTTGTTTATAG GTTGAGTATATTTCAATGGTTTTTGAAAAATCTATGTAAATAAAAAAAATACAATTATTTATATA AAATAAAATAATCTTTTTTTTATTATTATGACATTGATGAGAGTATCTAATAATTTGACCCATAA CTAATTTGGATAAAAAAAAATCTGATTGACCACTTTTAATTTAATGTATCACTAAACTAAATACC CTTTTTTAAAATAGTCTAAACATGAATTAAATATTCAAAAGAAATATTTTACTTGAGATTATTAC CTAATATTAATGATAATTTCATTCAACTCCAATAAAATTAATTTTCATGTAAGATATATCTAAAA GAAAAGATATATATAAATTTTATTTTCACTAGTAAAAAAAAGTTGATCTAGTTAGTGAAAAACCA ACTCATATCCTATAAGAATATGAATTTGATTTTTTTTGTTAAGGTGAGAATTTTATTGATCAATA ATTTATAAATATCTATATAAATAATCTTTAGCCTTATGAGTCCTTAGGTCAATTCAACTCACCTA AATTTTTTATTATGAAAAAAAAAATTGTATCTTCACAAGATAAATGTGTTGGATTCAATCACTCC TTATTAGCTTAATTAGATTATAATTGTAGTCCCCTATATATATATATGTATCATCTTGTCAAATA ATAATGAAATATAGAATTTATTTAGACTTAGAGAATAAAATTAAAAACTGTCTGCCATGAAAAAA GACGAAGTTAAGAAAAGGGCCAATCATAGAAGATTTTTATGGGCACTTCACGGACACTAACTCAC TGTCACAATCATCACTGGGGTTGACAAAAGGACAATATGAAACACTTTTGAGAAGCATGTACCAC TCATCCATTTATCAGTGGCTCCCAATTCCCAGAGGCCAGAACTATATATGAAAGAATTGTTGAAC GCACGGGCATGAACCCATTCTTGAAGCATCATTGTGTGAGAATATCTTGACCTTGTAAGATGCAA CACCTTTTTAAGCCTTAAATTTAAAAAAGGAAAAAAGAAAAATCTTGTCTCTACTTTCTTTTAGC ACAAGTGTATAGAAATTCTTAAATATATACACTCTCCTTTATATTGTAGTATCAGTGGCGCAAAT CATTATATTTCATTTTTAATAATAAAATTAAGAGCATTAATTTTATAGTTAAAATTGAAAATAAA GATAATTTACAGAACTCATTTGACTTAAACTGACAAAATATATATATATATATATATATATATAT ATATATATATTGTGAGATGAACATGTTACTTTTTTAACATGCAAAAAGGAGAATATATTTTACAT GCATGCACCCATGATAACTTCTATGTATATATCCATACAATACATCGTTCGTATATCGTCTCGTT TGTCTTTATTCTCCTCTCAAAATACGACAATAGCAATTTACATTTTTTTTTATAAGCAAATAGTA ATTTACATTTAACTTAGTAATGTAGGGATCGAACATAACCACTTGCGATGAATAATTTGCTTTAA ATTTTTGTTGATGCGTACCCTTTAACTGTCACTCATGGAATACGATTCTTCAATATCTAGTGCTT GATCGTTGACAGAACAACTTAAAACAGAGAATTGATGTTTTGGCTTTTCCATGGATAATAATTAT CCCAGTGACATACCAAAGCATAGTAGCTAAGAAGACTTTCACGTAAAAAAAAGTTTCTTTTATTC CCTTTTTTAATTTGGTGAATCACAAAAAACCACTTTTGTTTGGTTCCAAAGTTAGGCTCATGGAA AGTTTAAACCTCCATAGAATGGTCACGAAGAGATTGCATCTTTGTCTTCACAAAAGCTAACTCCA CGTTGAGTAGACTTAACAGCCAGTGGCGAATAGCAAGGATATTTCATTAATTATACGCCACCGGC CAAATGTTAACCAATCGTATTATAATTAAGTTCCATCATCATCAAACAATTTAGTAAAGTGCATG ACCCAAATTTCTACGATACATATTTATTTATTAAAAATGTAAGAATATTTCAGTCATATTTAAAA ATATATATATCAAGAATAATTAACTTTGTACACACGCACTGAATAAAAGATTTGTGACAGACAAG GCTTGCATAAAAATTTCTCCTCTAAACTAATTGCTTGTAGGACCTCTCCCACCACTATAGAATCA ATATAATTAATCCGCATTAGAAAGTTATATTGTATACAATTTTCTTGAAACATAATTATACTTCA TGTTTCACAGACTTATAGTGGATCTTGTGTGGCTAGCTACTGATGAATATTGTTTTTTTTTTTTC CTAAGCATCCACTTTGAACAACTTTTCCCATTTCATACAAACAGAATTAATTAGTATTGCGTGCC ACCATATGGTACAGTGTTGTACATGCATATAAGCTATTTAATTTAATAATATACAAACATAACGG TGTATATAAATAGAGGCAGCATGTGGTGTGTGGTGTAAAAATAAGGACGCAGGCAAATGTATGCA TTTGGCATAAGTATATAAGAGAGAGGGAGTAGTACTACTGCAAAGCAAAATCAGAGAGACATGAG AAGCTGTGTGTGCTACACGCTATTATTGTTTATTTTCTTCATATGGCTGCGCGTGGCAACGTGCT CTTCGTTCACTGACATGGAATCGCTTCTGAAGCTGAAGGACTCCATGAAAGGAGATAAAGCCAAA GACGACGCTCTCCATGACTGGAAGTTTTTCCCCTCGCTTTCTGCACACTGTTTCTTTTCAGGCGT AAAATGCGACCGAGAACTTCGAGTCGTTGCTATCAACGTCTCGTTTGTTCCTCTCTTCGGTCACC TTCCGCCGGAGATCGGACAATTGGACAAACTCGAGAACCTCACCGTCTCGCAGAACAACCTCACC GGCGTACTTCCCAAGGAGCTCGCCGCCCTCACTTCCCTCAAGCACCTCAACATCTCTCACAACGT CTTCTCCGGCCATTTCCCCGGCCAAATTATCCTTCCGATGACGAAACTGGAGGTCCTCGACGTCT ACGACAACAACTTCACCGGACCGCTTCCCGTAGAGTTGGTGAAACTGGAGAAATTAAAATACCTG AAGCTCGACGGAAACTATTTCTCCGGCAGCATACCGGAGAGTTACTCGGAGTTTAAGAGCTTGGA GTTTTTAAGCTTAAGCACCAATAGCTTATCGGGGAAGATTCCCAAGAGTTTGTCGAAGTTGAAGA CGCTGAGGTACCTAAAACTCGGATACAACAACGCTTACGAAGGTGGAATTCCACCGGAGTTTGGC AGCATGAAATCTCTGAGATACCTTGACCTCTCTAGCTGCAACCTCAGCGGCGAGATTCCACCGAG CCTTGCAAATCTGACAAACCTTGACACGTTGTTCCTGCAAATTAACAACCTCACCGGAACCATTC CGTCGGAGCTCTCCGCTATGGTGAGCCTCATGTCACTTGATCTCTCCATCAACGACCTCACCGGT GAGATACCGATGAGCTTCTCACAGCTTAGAAACCTCACTCTCATGAACTTCTTCCAAAACAATCT TCGCGGCTCAGTTCCGTCCTTCGTCGGCGAGCTTCCGAATCTGGAAACGCTGCAGCTCTGGGATA ACAACTTCTCCTTCGTGCTACCTCCGAACCTTGGGCAAAACGGCAAGTTAAAGTTCTTCGACGTC ATCAAGAATCACTTCACCGGGTTGATCCCTCGAGATTTGTGTAAGAGTGGGAGGTTACAAACGAT CATGATCACAGATAACTTCTTCCGCGGTCCAATCCCTAACGAGATTGGTAACTGCAAGTCTCTCA CCAAGATCCGAGCCTCCAATAACTACCTTAACGGCGTGGTTCCGTCAGGGATTTTCAAACTACCT TCTGTCACGATAATCGAGCTGGCCAATAACCGTTTTAACGGCGAACTGCCTCCTGAGATTTCCGG CGAATCCCTGGGGATTCTCACTCTTTCCAACAACTTATTCAGTGGGAAAATTCCCCCAGCGTTGA AGAACTTGAGGGCACTGCAGACTCTCTCACTTGACGCAAACGAGTTCGTTGGAGAAATACCGGGA GAGGTTTTTGACCTACCGATGCTGACTGTGGTCAACATAAGCGGCAACAATCTAACCGGACCAAT CCCAACGACGTTGACTCGCTGCGTTTCACTCACCGCCGTGGACCTCAGCCGGAACATGCTTGAAG GGAAGATTCCGAAGGGAATCAAAAACCTCACGGACTTGAGCATTTTCAATGTGTCGATAAACCAA ATTTCAGGGCCAGTCCCTGAGGAGATTCGCTTCATGTTGAGTCTCACCACATTGGATCTATCCAA CAACAATTTCATCGGCAAGGTCCCAACCGGGGGTCAGTTCGCGGTCTTCAGCGAGAAATCCTTTG CAGGGAACCCCAACCTCTGTACCTCCCACTCTTGCCCGAATTCCTCGTTGTACCCTGACGACGCC TTGAAGAAGAGGCGCGGCCCTTGGAGTTTGAAATCCACGAGGGTGATAGTCATCGTGATTGCACT GGGCACAGCCGCGCTGCTGGTGGCGGTGACGGTGTACATGATGAGGAGGAGGAAGATGAACCTTG CGAAGACGTGGAAGCTGACGGCGTTCCAGCGGCTGAACTTCAAAGCCGAGGACGTGGTGGAGTGT CTGAAGGAGGAGAACATAATAGGAAAAGGAGGGGCAGGGATCGTGTACCGCGGGTCCATGCCAAA CGGAACAGACGTGGCGATAAAGCGGTTGGTTGGGGCGGGGAGTGGAAGGAACGATTACGGATTCA AAGCGGAGATAGAAACGCTGGGGAAGATAAGGCACAGGAACATAATGAGGCTTTTAGGTTACGTG TCGAACAAGGAGACGAACTTGCTGCTGTATGAGTACATGCCAAATGGGAGCTTAGGGGAATGGCT GCATGGTGCCAAAGGAGGGCACTTGAAGTGGGAAATGAGGTACAAGATTGCGGTGGAAGCTGCTA AGGGACTGTGCTATTTGCACCATGATTGTTCCCCTCTTATCATTCACAGGGATGTCAAGTCTAAT AATATATTGCTGGATGGGGACTTGGAGGCCCATGTTGCTGATTTTGGCCTTGCCAAGTTCTTGTA CGACCCTGGCGCCTCTCAGTCCATGTCCTCCATTGCTGGCTCCTACGGCTACATTGCTCCAGGTT CCATTCATTATTATTTTCTCTTTTCCTTCTTCATAATCTTAATATACCATGCAGATAACGTACAA CATGCATACTTATACATATAATTTTATCCTTTCAACATATAATCAAATATTTCATATCTAATAAT ACCAACTTCATATTATAAACATCACCTAATATAATCAACATGACTTGATAAATAAGACATATAAG TTCAATATTTAAACTCATGTGTCTGAAAAAACATTAATTGGAAAAGTCACTCTTAAAAATATTTG ATAATATATCAATATGACCATATGATTCCAATTACGATCACAAACTCTGTTAAAAATTCTTGCTG AAGATATTAGTCCTTGAATACTAATATAAGAATATCTTGGGTTAGAAAAGTTACTATTTTACTGT TAATTCCCGTTTACTTTAGATGGGTTGGAAGTTGAAAAGTTGAGTGATTTAATTTGTTTCTGGTG GTTGCGCAGAGTATGCATACACTTTGAAAGTGGACGAGAAAAGTGATGTGTACAGCTTTGGCGTT GTGCTGCTGGAGCTGATAATAGGGAGGAAGCCAGTGGGAGAGTTTGGAGACGGGGTGGACATCGT TGGATGGGTCAACAAAACGAGATTGGAGCTCGCTCAGCCGTCGGATGCAGCGTTGGTGTTGGCAG TGGTGGACCCAAGGTTGAGTGGGTATCCATTGACAAGTGTCATTTACATGTTCAACATAGCTATG ATGTGTGTTAAAGAAATGGGGCCCGCTAGGCCTACCATGAGGGAAGTCGTTCATATGCTCTCAGA GCCTCCTCACTCTGCTACTCACACTCACAACCTAATTAATCTCTAGTTAATTAAGTTATTTGCTC ATCGATCCAGAATCACTTCTTTTCAAAATAAATTAACACAGACGAAAACTGTAGGAATAACTTTC ATCTGTTGTTTGTCGGAAGTGAAACAACGAATCAAATGTGAACTATGTATCAAATGTAAGATAGG TTTTAATTAATTTTGTAATATTGGTGTCAACTGTCAAGTAATTCGAAGGATTTTCCCCATTGTGC ATGTATCAAGA GmNARK: ATGAGAAGCTGTGTGTGCTACACGCTATTATTGTTTATTTTCTTCATATGGCTGCGCGTGGCAAC Glyma12g04390 GTGCTCTTCGTTCACTGACATGGAATCGCTTCTGAAGCTGAAGGACTCCATGAAAGGAGATAAAG cDNA (SEQ ID CCAAAGACGACGCTCTCCATGACTGGAAGTTTTTCCCCTCGCTTTCTGCACACTGTTTCTTTTCA NO: 39) Soybean GGCGTAAAATGCGACCGAGAACTTCGAGTCGTTGCTATCAACGTCTCGTTTGTTCCTCTCTTCGG CLV1-like gene TCACCTTCCGCCGGAGATCGGACAATTGGACAAACTCGAGAACCTCACCGTCTCGCAGAACAACC TCACCGGCGTACTTCCCAAGGAGCTCGCCGCCCTCACTTCCCTCAAGCACCTCAACATCTCTCAC AACGTCTTCTCCGGCCATTTCCCCGGCCAAATTATCCTTCCGATGACGAAACTGGAGGTCCTCGA CGTCTACGACAACAACTTCACCGGACCGCTTCCCGTAGAGTTGGTGAAACTGGAGAAATTAAAAT ACCTGAAGCTCGACGGAAACTATTTCTCCGGCAGCATACCGGAGAGTTACTCGGAGTTTAAGAGC TTGGAGTTTTTAAGCTTAAGCACCAATAGCTTATCGGGGAAGATTCCCAAGAGTTTGTCGAAGTT GAAGACGCTGAGGTACCTAAAACTCGGATACAACAACGCTTACGAAGGTGGAATTCCACCGGAGT TTGGCAGCATGAAATCTCTGAGATACCTTGACCTCTCTAGCTGCAACCTCAGCGGCGAGATTCCA CCGAGCCTTGCAAATCTGACAAACCTTGACACGTTGTTCCTGCAAATTAACAACCTCACCGGAAC CATTCCGTCGGAGCTCTCCGCTATGGTGAGCCTCATGTCACTTGATCTCTCCATCAACGACCTCA CCGGTGAGATACCGATGAGCTTCTCACAGCTTAGAAACCTCACTCTCATGAACTTCTTCCAAAAC AATCTTCGCGGCTCAGTTCCGTCCTTCGTCGGCGAGCTTCCGAATCTGGAAACGCTGCAGCTCTG GGATAACAACTTCTCCTTCGTGCTACCTCCGAACCTTGGGCAAAACGGCAAGTTAAAGTTCTTCG ACGTCATCAAGAATCACTTCACCGGGTTGATCCCTCGAGATTTGTGTAAGAGTGGGAGGTTACAA ACGATCATGATCACAGATAACTTCTTCCGCGGTCCAATCCCTAACGAGATTGGTAACTGCAAGTC TCTCACCAAGATCCGAGCCTCCAATAACTACCTTAACGGCGTGGTTCCGTCAGGGATTTTCAAAC TACCTTCTGTCACGATAATCGAGCTGGCCAATAACCGTTTTAACGGCGAACTGCCTCCTGAGATT TCCGGCGAATCCCTGGGGATTCTCACTCTTTCCAACAACTTATTCAGTGGGAAAATTCCCCCAGC GTTGAAGAACTTGAGGGCACTGCAGACTCTCTCACTTGACGCAAACGAGTTCGTTGGAGAAATAC CGGGAGAGGTTTTTGACCTACCGATGCTGACTGTGGTCAACATAAGCGGCAACAATCTAACCGGA CCAATCCCAACGACGTTGACTCGCTGCGTTTCACTCACCGCCGTGGACCTCAGCCGGAACATGCT TGAAGGGAAGATTCCGAAGGGAATCAAAAACCTCACGGACTTGAGCATTTTCAATGTGTCGATAA ACCAAATTTCAGGGCCAGTCCCTGAGGAGATTCGCTTCATGTTGAGTCTCACCACATTGGATCTA TCCAACAACAATTTCATCGGCAAGGTCCCAACCGGGGGTCAGTTCGCGGTCTTCAGCGAGAAATC CTTTGCAGGGAACCCCAACCTCTGTACCTCCCACTCTTGCCCGAATTCCTCGTTGTACCCTGACG ACGCCTTGAAGAAGAGGCGCGGCCCTTGGAGTTTGAAATCCACGAGGGTGATAGTCATCGTGATT GCACTGGGCACAGCCGCGCTGCTGGTGGCGGTGACGGTGTACATGATGAGGAGGAGGAAGATGAA CCTTGCGAAGACGTGGAAGCTGACGGCGTTCCAGCGGCTGAACTTCAAAGCCGAGGACGTGGTGG AGTGTCTGAAGGAGGAGAACATAATAGGAAAAGGAGGGGCAGGGATCGTGTACCGCGGGTCCATG CCAAACGGAACAGACGTGGCGATAAAGCGGTTGGTTGGGGCGGGGAGTGGAAGGAACGATTACGG ATTCAAAGCGGAGATAGAAACGCTGGGGAAGATAAGGCACAGGAACATAATGAGGCTTTTAGGTT ACGTGTCGAACAAGGAGACGAACTTGCTGCTGTATGAGTACATGCCAAATGGGAGCTTAGGGGAA TGGCTGCATGGTGCCAAAGGAGGGCACTTGAAGTGGGAAATGAGGTACAAGATTGCGGTGGAAGC TGCTAAGGGACTGTGCTATTTGCACCATGATTGTTCCCCTCTTATCATTCACAGGGATGTCAAGT CTAATAATATATTGCTGGATGGGGACTTGGAGGCCCATGTTGCTGATTTTGGCCTTGCCAAGTTC TTGTACGACCCTGGCGCCTCTCAGTCCATGTCCTCCATTGCTGGCTCCTACGGCTACATTGCTCC AGAGTATGCATACACTTTGAAAGTGGACGAGAAAAGTGATGTGTACAGCTTTGGCGTTGTGCTGC TGGAGCTGATAATAGGGAGGAAGCCAGTGGGAGAGTTTGGAGACGGGGTGGACATCGTTGGATGG GTCAACAAAACGAGATTGGAGCTCGCTCAGCCGTCGGATGCAGCGTTGGTGTTGGCAGTGGTGGA CCCAAGGTTGAGTGGGTATCCATTGACAAGTGTCATTTACATGTTCAACATAGCTATGATGTGTG TTAAAGAAATGGGGCCCGCTAGGCCTACCATGAGGGAAGTCGTTCATATGCTCTCAGAGCCTCCT CACTCTGCTACTCACACTCACAACCTAATTAATCTCTAG GmNARK: MRSCVCYTLLLFIFFIWLRVATCSSFTDMESLLKLKDSMKGDKAKDDALHDWKFFPSLSAHCFFS Glyma12g04390 GVKCDRELRVVAINVSFVPLFGHLPPEIGQLDKLENLTVSQNNLTGVLPKELAALTSLKHLNISH protein (SEQ NVFSGHFPGQIILPMTKLEVLDVYDNNFTGPLPVELVKLEKLKYLKLDGNYFSGSIPESYSEFKS ID LEFLSLSTNSLSGKIPKSLSKLKTLRYLKLGYNNAYEGGIPPEFGSMKSLRYLDLSSCNLSGEIP NO: 40) Soybean PSLANLTNLDTLFLQINNLTGTIPSELSAMVSLMSLDLSINDLTGEIPMSFSQLRNLTLMNFFQN CLV1-like gene NLRGSVPSFVGELPNLETLQLWDNNFSFVLPPNLGQNGKLKFFDVIKNHFTGLIPRDLCKSGRLQ TIMITDNFFRGPIPNEIGNCKSLTKIRASNNYLNGVVPSGIFKLPSVTIIELANNRFNGELPPEI SGESLGILTLSNNLFSGKIPPALKNLRALQTLSLDANEFVGEIPGEVFDLPMLTVVNISGNNLTG PIPTTLTRCVSLTAVDLSRNMLEGKIPKGIKNLTDLSIFNVSINQISGPVPEEIRFMLSLTTLDL SNNNFIGKVPTGGQFAVFSEKSFAGNPNLCTSHSCPNSSLYPDDALKKRRGPWSLKSTRVIVIVI ALGTAALLVAVTVYMMRRRKMNLAKTWKLTAFQRLNFKAEDVVECLKEENIIGKGGAGIVYRGSM PNGTDVAIKRLVGAGSGRNDYGFKAEIETLGKIRHRNIMRLLGYVSNKETNLLLYEYMPNGSLGE WLHGAKGGHLKWEMRYKIAVEAAKGLCYLHHDCSPLIIHRDVKSNNILLDGDLEAHVADFGLAKF LYDPGASQSMSSIAGSYGYIAPEYAYTLKVDEKSDVYSFGVVLLELIIGRKPVGEFGDGVDIVGW VNKTRLELAQPSDAALVLAVVDPRLSGYPLTSVIYMFNIAMMCVKEMGPARPTMREVVHMLSEPP HSATHTHNLINL GmCLV1A: AGCTTCGCATAAGTAACGTGAGTTTAGTTAAGTCGAGCTAGTCGCCTTTTTCTATGGTTGGTTAT Glyma11g12190 GTGCAGTAGTGAATGTTGTGTAGTATCTTGCGAGGCCATGTTTGGTGTGACAAGCCCGAAAGTGA gDNA + about CTTGAGGGGAACAAAATAGCTTTTGTCCAAACATGCTAACTTGTCATCATGACATCTACTTCTCT 1.6 kb promoter GGTCATGGCAGCTCTGATTAATAATTTAAGTGATCATAATATTAGAAGTTAAAAAATTATAACAT and 5′UT CTTTAATTATTTTTATTATTTTATATAATCTTAAAAATTATTTCAAACTTCTTTAAACAATGTTG sequence (SEQ AATAAGATCATGTATTTTTTTTTTTTCCTTACGTAGTAGTATCCTGGCAGTCACCCAGGAGCAAA ID TGATGTAGATAAATCCTTTTTACTAAAATAGTCTTGGAGCAATATTTAAGAGGGGACCATTTTAT NO: 41) Soybean GATCTTTTCTATCTTAATAGTGGCGTTAGAATAACACTTTTTTAAGCTTTAAATAAAAAATAAAA CLV1-like gene AAATATTATCTTTACTTTCTTTTAGCAATTATTCCTACGTGTAGAGAAACTGTTAAATACACTCT CCTTTGTATTGTATAATGTTGCATTGTATCAGTTGTCCAAATTAATCACAGTATATTAGTAATAA AATTATGAACATTAATTTTATTCTTAAAATTTAGTTAAATATTGATAATTCACATAACTCGTGAC TTAATCTAATTATATATAGAAGATCATGTTAGTATGTTACCTTTTTAAAATGCAAAATGAAGAAT CTGTTACATGCACCCACTGGGTAAGTTGATAACTATTATGTATCTATATATCGTCTGGATATTGT CTGTTTCATTCTCTTCTCAAAAGAACAAAATGGTAATTTACATTTAACTTAGAAATGTTTGGGAC AGAATCACTAGCTTGCAGATGAATAATTTGTTTTAAATTCTTATTGATGCATAATACCCTCTACT TGTCACTCATAGAATACGATTCTGCAATAACTAGTGCTTGATCCTTGACAGAACAAATTAAAACA GAGAATTGATGCATTGGCTTTTCCATGGACAATAATTATCCCATTGATGTACTAAAGCACAGTAA CTAGGTAGGAAGACCTCCACCTAAAGAAACTTTCTTTTATTCTCCTTTAATTTTAAATTTGGTGA ATCACTTAAAACAACTTTTGTTTCATTCCAAAGTTAGGCTCATGGAAAGCTTAAACCTAGTTAAA TAGCCACGAAAGAGATTGCATCTTTGTTTTCACAAAAGCTAACTGCGCGTTTGTGAAGCTAGTGA TGCATAGTATATATATATTTTTTTCTCGGCATCCACTTTGAGAACTACTTTTTTTTTCATTTTCA TAGAAACAGAATTGAAGTAGTATAACATGCCACCATGAACAGTACAGTGATGTACATGAATAAAT GCATGCTATTCAATATAATGTATAATATAACGGTGTATATATAAATAGAGACTGCATGAGGTGTG TGGTGTCAACATATAATAAGGACGCAGCGTAGGTATAATAGTGAGTACCGCGAAGAAAGATAAGA GCCAGAGCCATGAGAAGCTGTGTGCTTTACACGCTATTATTGTTTGTTTTCTGCATATGGGTTCC CATGGCAACGTGCTCTTCGTTCAGTGACATGGATGCGTTACTAAAGCTGAAGGAGTCCATGAAAG GAGACGAAGCCAAAGACGACGCACTCCATGACTGGAAGTTTTCCACATCGCATTCTGCACACTGT TTCTTTTCAGGCGTAACATGTGACCAAGACCTTCGAGTCGTTGCTATCAACGTCTCCTTTGTTCC TCTCTTCGGTCACATTCCGCCGGAGATCGGAAACTTGGACAAGCTGGAAAATCTCACAATCGTGA ACAACAATCTAACCGGTGTACTCCCCATGGAGCTTGCCGCCCTCACTTCCCTCAAGCACCTCAAC ATATCTCACAACCTCTTCACCGGCGATTTCCCCGGCCAAGCCACTCTTCCGATGACGGAACTTCA AGTCCTCGACGTCTACGACAACAACTTCACCGGACCGCTTCCGGAAGAATTCGTGAAACTGGAGA AACTAAAATACCTGAAACTCGACGGAAACTATTTTACCGGCAGCATACCGGAGAGTTACTCGGAG TTTAAGAGCTTGGAGTTTTTGAGCTTAAACACCAACAGCTTATCGGGGAGGATTCCGAAGAGTTT GTCCAAGTTGAAGACTCTGAGGATTCTCAAACTCGGATACAGCAACGCTTACGAAGGTGGAATTC CTCCGGAGTTCGGCACCATGGAATCTCTGAGATTCCTCGACCTCTCAAGCTGCAACCTCAGCGGC GAGATTCCACCGAGTCTTGCAAATCTGACAAACCTAGACACGTTGTTCTTGCAAATGAACTTCCT CACCGGAAGCATTCCGTCTGAACTCTCTTCTTTGGTGAGGCTCATGGCACTGGATCTCTCCTGCA ACAGCCTCACCGGGGAGATTCCAGAGAGCTTTTCTCAGCTGAGAAACCTCACTCTCATGAACTTG TTCCGCAACAATCTTCACGGCCCTATTCCGTCCTTGCTGAGCGAGCTTCCCAATCTGAATACGCT GCAGCTCTGGGAGAATAACTTCTCCTCTGAGCTCCCGCAGAACCTGGGGCAAAACGGGAGGCTGA AGTTCTTCGACGTCACGAAGAATCACTTCAGCGGGTTGATCCCTCGGGATTTGTGCAAGAGTGGG AGGTTACAAATCTTCATTATCACAGATAACTTCTTTCATGGCCCAATCCCTAACGAGATTGCTAA CTGCAAGTCTCTAACCAAGATCCGAGCCTCCAATAACTACCTTAACGGCGCAGTTCCGTCGGGGA TTTTCAAGCTACCTTCCGTCACGATAATCGAGTTGGCCAATAACCGTTTTAACGGAGAACTGCCT CCCGAAATTTCCGGCGATTCACTCGGGATTCTCACTCTTTCCAACAACTTATTCACTGGGAAAAT TCCCCCAGCGTTGAAGAACTTAAGGGCACTGCAGACTCTGTCACTTGACACGAACGAGTTCCTTG GAGAAATCCCGGGGGAGGTTTTTGACCTACCAATGCTGACTGTGGTCAACATAAGCGGCAACAAT CTCACCGGACCAATCCCAACGACGTTTACTCGCTGCGTTTCACTCGCCGCCGTTGATCTCAGCCG GAACATGCTAGTTGAGGATATTCCTAAGGGGATTAAGAACCTCACGGTCTTGAGCTTTTTCAATG TCTCGAGAAACCATTTAACAGGGCCAGTCCCTGACGAGATAAAATTCATGACGAGCCTCACCACG CTGGATCTCTCCTACAACAATTTCACAGGCAAGGTCCCCAACGAGGGTCAGTTTTTGGTCTTCAA CGACAACTCGTTTGCAGGGAACCCTAACCTCTGTTCCATTCACGGATGCACTTTAAGCATTGTGG GGGCAGCTGCCCCTATCAACATTTTAACATTTGTAAATATAGTATGTACAATTATAGTAATTTAT AAATTGCTTGTATAA GmCLV1A: ATGGCAACGTGCTCTTCGTTCAGTGACATGGATGCGTTACTAAAGCTGAAGGAGTCCATGAAAGG Glyma11g12190 AGACGAAGCCAAAGACGACGCACTCCATGACTGGAAGTTTTCCACATCGCATTCTGCACACTGTT cDNA (SEQ ID TCTTTTCAGGCGTAACATGTGACCAAGACCTTCGAGTCGTTGCTATCAACGTCTCCTTTGTTCCT NO: 42) Soybean CTCTTCGGTCACATTCCGCCGGAGATCGGAAACTTGGACAAGCTGGAAAATCTCACAATCGTGAA CLV1-like gene CAACAATCTAACCGGTGTACTCCCCATGGAGCTTGCCGCCCTCACTTCCCTCAAGCACCTCAACA TATCTCACAACCTCTTCACCGGCGATTTCCCCGGCCAAGCCACTCTTCCGATGACGGAACTTCAA GTCCTCGACGTCTACGACAACAACTTCACCGGACCGCTTCCGGAAGAATTCGTGAAACTGGAGAA ACTAAAATACCTGAAACTCGACGGAAACTATTTTACCGGCAGCATACCGGAGAGTTACTCGGAGT TTAAGAGCTTGGAGTTTTTGAGCTTAAACACCAACAGCTTATCGGGGAGGATTCCGAAGAGTTTG TCCAAGTTGAAGACTCTGAGGATTCTCAAACTCGGATACAGCAACGCTTACGAAGGTGGAATTCC TCCGGAGTTCGGCACCATGGAATCTCTGAGATTCCTCGACCTCTCAAGCTGCAACCTCAGCGGCG AGATTCCACCGAGTCTTGCAAATCTGACAAACCTAGACACGTTGTTCTTGCAAATGAACTTCCTC ACCGGAAGCATTCCGTCTGAACTCTCTTCTTTGGTGAGGCTCATGGCACTGGATCTCTCCTGCAA CAGCCTCACCGGGGAGATTCCAGAGAGCTTTTCTCAGCTGAGAAACCTCACTCTCATGAACTTGT TCCGCAACAATCTTCACGGCCCTATTCCGTCCTTGCTGAGCGAGCTTCCCAATCTGAATACGCTG CAGCTCTGGGAGAATAACTTCTCCTCTGAGCTCCCGCAGAACCTGGGGCAAAACGGGAGGCTGAA GTTCTTCGACGTCACGAAGAATCACTTCAGCGGGTTGATCCCTCGGGATTTGTGCAAGAGTGGGA GGTTACAAATCTTCATTATCACAGATAACTTCTTTCATGGCCCAATCCCTAACGAGATTGCTAAC TGCAAGTCTCTAACCAAGATCCGAGCCTCCAATAACTACCTTAACGGCGCAGTTCCGTCGGGGAT TTTCAAGCTACCTTCCGTCACGATAATCGAGTTGGCCAATAACCGTTTTAACGGAGAACTGCCTC CCGAAATTTCCGGCGATTCACTCGGGATTCTCACTCTTTCCAACAACTTATTCACTGGGAAAATT CCCCCAGCGTTGAAGAACTTAAGGGCACTGCAGACTCTGTCACTTGACACGAACGAGTTCCTTGG AGAAATCCCGGGGGAGGTTTTTGACCTACCAATGCTGACTGTGGTCAACATAAGCGGCAACAATC TCACCGGACCAATCCCAACGACGTTTACTCGCTGCGTTTCACTCGCCGCCGTTGATCTCAGCCGG AACATGCTAGTTGAGGATATTCCTAAGGGGATTAAGAACCTCACGGTCTTGAGCTTTTTCAATGT CTCGAGAAACCATTTAACAGGGCCAGTCCCTGACGAGATAAAATTCATGACGAGCCTCACCACGC TGGATCTCTCCTACAACAATTTCACAGGCAAGGTCCCCAACGAGGGTCAGTTTTTGGTCTTCAAC GACAACTCGTTTGCAGGGAACCCTAACCTCTGTTCCATTCACGGATGCACTTTAAGCATTGTGGG GGCAGCTGCCCCTATCAACATTTTAACATTTGTAAATATAGTATGTACAATTATAGTAATTTATA AATTGCTTGTATAA GmCLV1A: MATCSSFSDMDALLKLKESMKGDEAKDDALHDWKFSTSHSAHCFFSGVTCDQDLRVVAINVSFVP Glyma11g12190 LFGHIPPEIGNLDKLENLTIVNNNLTGVLPMELAALTSLKHLNISHNLFTGDFPGQATLPMTELQ protein (SEQ VLDVYDNNFTGPLPEEFVKLEKLKYLKLDGNYFTGSIPESYSEFKSLEFLSLNTNSLSGRIPKSL ID SKLKTLRILKLGYSNAYEGGIPPEFGTMESLRFLDLSSCNLSGEIPPSLANLTNLDTLFLQMNFL NO: 43) Soybean TGSIPSELSSLVRLMALDLSCNSLTGEIPESFSQLRNLTLMNLFRNNLHGPIPSLLSELPNLNTL CLV1-like gene QLWENNFSSELPQNLGQNGRLKFFDVTKNHFSGLIPRDLCKSGRLQIFIITDNFFHGPIPNEIAN CKSLTKIRASNNYLNGAVPSGIFKLPSVTIIELANNRFNGELPPEISGDSLGILTLSNNLFTGKI PPALKNLRALQTLSLDTNEFLGEVPGEVFDLPMLTVVNISGNNLTGPIPTTFTRCVSLAAVDLSR NMLVEDIPKGIKNLTVLSFFNVSRNHLTGPVPDEIKFMTSLTTLDLSYNNFTGKVPNEGQFLVFN DNSFAGNPNLCSIHGCTLSIVGAAAPINILTFVNIVCTIIVIYKLLV Glyma18g51820 GCCTGCCCCTTAGTCATGTGCAAAATAGTGCTAAGATCTGTATTGTAAAATGGCCACATTGGTCT gDNA + about TAGTAAAAGAGTTATGCATATGCTGCACTGGTAGCACCCAGCCTGCACTTCGTAATATGATGATT 3.7 kb promoter GTGTATTTTTGTTTACTTTTGAGGTGAAGCTGCGATGCATTAGGCTAGGGATTTGTGTATGTTGT and 5′UT (SEQ GTACATTGGTTTTTGTGAAGGTGTTGTTGTGGCTGTAATTTACATTTTTGTATTTTTGGGATTAC ID TTGGTGGGACATGTGCTGAGGATGCCATGTCCCTAGTTCTCTAATGTTCTGATGTATTATTTATT NO: 44) Soybean TATATTGATAAAAAAAATTATATACTTTCAAAGGCAAAAAGATAAAGAAAACTATCAATCACCTG CRN-like gene CTATTTTAGAAATACCCCCCTCCCAAAAGAAAAACCCAAATTATTGTAATCATATAAAGTTTCGG TGTTGAAAAGACGGCGTGGGGCACCATGTTGAAGGCTTGAGAATTTTTTGGTCAATTGAATCAAA AAGTGAAGTGGTCCATTTGACCCCCAGTTTGCAATGGTAAATTCAAGAATTGGGTGGAAGTGTCC ATTGTATTTTTCGTATCCAACAATAAAGAATCACAGTTGTTGCACAGATACAACAATCAAAGGTC TAGATATTTTGTAGTCTTATAATAGGAATTTTCACTGTTTTACACAAACATTTTTTTATCTACAA AACAAACCGTGAGGAATCTTGTAGGTTATAGTGGCCAACACTCATGTTGCGTTAACACAGCTATC AACTAAAACTCAACTTTTGTCACGGGTGACCTCAACATAATTATTGATATTACTGACAGAGTAAC AACACCTGAAGTGGGCCCTGTTGAACTGGGTTATGACTAATGACGAGACCACAACTTAGAGGATA GATACATTTCTAATCTTTCAAATAAATACAAGTGATATTAACTTGGTCTTTGAAAAATATGAACA TCAATTCTGTTTTTTAATTATAAAAACAATAGTAATTTGATTTGATTTACTGAAAAAATTAGCGT CAATTTAAAATTTCAGTATTAAAAAATGATACGATTTACCATCTTAAGTATTGCACGCAAAAGAT TATTTTAATATCATTTTTCAATTATTAAGAGAAAAAAAGTGACGTTAATATCTTAGGACAAAATT AATATCTCTGGCATTTTAAGAAAATAAAGAGAATACTTATAAAATAAGACCACAATTCACGAAAT CTTATATTAAATATGGTCCTGATAATTCCAATTTGTATAAACTTATTAAAATAATACTTATAGGG AAAAAATAGAGAGGCAAATAAATTAAAATCAAATTATGTATTTTTACTTTTGGAGAATTTAAATA AGAGAATTTCTTAAAACTTGAGTTAGATAAGTTGATTTTAATTTGTGGGAGATTCTTTTTTATTA TATGTCTTTATTTTTTTCTCAGTATTTTTTTTTTTTGGAAAATTTTACCTAAACTGAAATTAAGC ATTGTGGAGAATACTTTCAGGGAAAATGACTCAATGATTTAGCGTGTGATTTAAGCATAAATTTT GGTACAAGAGTTTGATTAACTATTAATTAAATTAATTTAGAAAGGTCAAGGTCATTTTCACACAA TTCTATTCCCTTGCTCGAGACCACTTTTCAAGTATAAATTTATGACTAATGGGTCAAAACATACA ATGCCTTGTGTAAATAGTTATGAACGATATTAATATTTTTATGAAAATGATAGTTGCACCAAATA TGTGAAATTCGCAATCTGAATTATCTGTTGCATTTGGCTTGGTTTCATTTTGTTAGGTTATTATT ATTATTTTTTTTTAAAAAGGAACTGACTGTATCCAATTATATGTCTGTTTTTAAAATTTGAAAGA AATAGTTTTAAACCATTTAATATAGCTATAATATATATTTAAGTTAATCTTAGCTATATATTTTG TATTAAAATGTATATTTGCTATAATAATTAACTCTAGTAATTTACCAAATGGATATTATTTGTAA AGGCTTGATTTGGGTTATACTAGTAATTTAAAATCTACGTACTTACTATTTCTGATTTCAAAATG TCTCATGCCACAAATGAACAAAACAATCATGATAATTTATTCATACTATTATTGCTTGCTCATTC ACTCACCCCACAGTGCTAGATCCTCGGACTCGAATAAATCATTTATTATGCTTAGATAATTCGAT TTATTTTTATTCAATGCAACACTCATTCAATTGCACTACCCTCCTATTCCTATATCACATTAATA TGAAGAGTTAATCTTATCCTCTCGATTCATTTTCTTTTTAAATTTAAGGGGTATAATGAGAAATT AATTTTGACTATTAAATTTTAAAAACAATCCAAAAATGTCATAAAGAATTTTTCCTATTCCACGA GAGAACTTGAAAGTTAAAATTTGATTAAAATCTTATTAAAGGCGTTCCTAATCCTAGCAACTTCC ACCTATCACAGAGAAAAAAAAAGGAAAAGAAAAGGTAAGATAGAAAGAAAGAAGGAAAAGTAAAA GCATGCAAATATAGAATTATAAATACTAAAAAATATTGTTAAGATATTAGTTAAAAAATTATTAA GATACACAAAATTACATTATACACAATTTTTTATAATCTTTAAAATAAATATTTTTTATTTTATT AATATCCTAAAGATATTAGTTAATTAACATTCATGTATTATTATTTGAAATTGAAACGTAAGTAG TAATTAAAAGCAAATTATTCTATCGAAAAAGAGATAACTTTATTAATGACACACACCAAACATAC CAATCGCTAGAGTTGTTAACCACTCACTCATATAGCATATCACAAATTCCCATGCAACCTTAATT CAACGGTCCAGATGCAGTCTGATGAGATCAGACGGTCGAGACGAACTGTACATTCTCCCTCTCAC GGATTTCGATGTTTCTCTTTCGGACCAAATGTGGGGCCCACATAGTACTGTGTCCTGAGTGCTGG CTACTCACAAAGGCGGGAACCAGTTTTTGTCGCAGAAGAGGTATGGCTCTTTGTTTGTTGTCATC AGATGAGAGAGAAACAAAACAAAGAGACAATCACTGAATCACTCTCACTCACTCTGCATGCTGTG TGCGTGACTCTGTCATTGTGTTTTGTGTTTTAAGCACTTTGCAGTTTAGTTTCTGAGGAGCGTTT TTTTTTTTTTCTTTCTTATGAGTGTGTGTCTGTTCTTAGTTGCTGTTATTGTTGTTCAAGTTTCG GTTACTACTACTACTACCACATGTCCATGCCCCTTCAATTTCTGTTCAACTTTGTGACTTTTTGT TTGGTTTCTAAGGAAAAAGATTGCAACTTGTTTCTGGGTCTAGTTTGCTTTTGGTTGGGTTTGTT AGTCACCGCTGGCAACTCGGAATAGTGGGTTTTTTTTTGGAGGGTGTTTTTTTTTTCTTCTTTTG GAGGTTCAAATTCTTGTTCTGATTCGTGTGAAGGTGGAAAATTTATGGGTGCTGAGAGGAGGAAA AAGATGGGATTTGGTGGAATAAATGTAAAACTATTCGGCGACAACATGTCTGCTTGCTTTTTTGG GACGGCTTTCTTGTGAAGATTTTGGGTTTAAAAGGTTGAGGAAGATGCTTATGCCTTATGCTTAT GCTTGCAACTTTTTTTTTAAAACCCATTTTAGCATCAAGTATAAAAGTTTCTTCTTGGTCTTGTT TCCAAGTGTTTGAGGTGATGGGGGTTTTGAGCATGTGAGTGATTCATGCCTCATTTTGGAGCTTC TGAGATTGGTTTCTGGTTGTGGCTTTGTTTGTTTTGTGTTGTGCTTTCATGTTTAGGAAAAGGCA CACCCTTTCTTCTCTTGCAAGGGAATTGTTGGCATTTCAGCCACTTTTTCTTCTCTTCTTGTTCA GCTTGCACCACAACACTATGCAGTGTCAAGGAAGGTTGAGTAAACATGTTTCTTCTGAGCCTCCC TCACCTTCTAGGTCAACACCATCACCACCATCTTCATCAGGATACAAGGATGACCCTAGGAAGAT AATTTTGAGCATGGTTTTAGGAGCAGTCACTGGACTAGTTTCTTCTGCTCTCTTTGCACTTGTGG TTCGTTGTGTTGTTCAGTATCTGAACCGCACACCAATCCTCAAGGGACCTGTCATATTCTCCCCC AAAATTGCCCCCATGACACTCCAATCAGCTTTGGCAAAGGAAAACCACTTGCTCGGTTCGAGTCC TAATGGGAAGTACTACAAAACTGTGCTTGACAATGGACTCACTATTGCAGTCAAAAGGCTAACAC CCTTTGAGAGTAATTCCCCGGAGGCTAAGAGAAAATCAGTGAAGAGGCAGATACAAACTGAGCTT GAGCTTCTTGCAAGCCTTAGGCATAGGAACTTGATGAGTTTAAGGGCCTATGTTCGTGAGCCTGA TGGGTTCTCATTGGTTTATGATTATGTTTCCACTGGGAGTCTTGCTGATGTGTTGAGTAAAGTGA GGGAGAATGAGTTGCCCTTTGGTTGGGAAGTTAGGCTCAGGATTGCTGTTGGTGTGGTGAAGGGT CTTCAGTATCTTCATTTCACTTGTGTGCCTCAGATTCTGCACTACAACTTGAAGCCCACAAATGT GATGTTGGATGCTGAGTTTGAACCTAGATTGGCAGATTATGGGTTGGCTAAACTTCTACCCAATT TGGATGGAGGAAGTTCTCTCTACACTCCTCCTGAATGTTTCCATAATTGCAGGTAAGACAAATTT CAATCATACTCATTCACTAGTGTTTTGAACTTGGTCTGTTTCTGTTCTTTCACTTTTTTACACCA ATAGGGTAATTAGGTGGTTGATATTGGGAATTTGTTTGATTCGTTACCTTTTCAAAAGCTCCACA CCTCATTGGTTTTTTGCCCCCTTTGTAGTACCCTAATGAAAGACTCTTGTTTTGAAACGAAATTA CTATTCTGTAATCTGTATTGTCATTGTATCATTTGCTGATTGAATTTGGTATTATTTAATAAAGA CTTTGCTATTTGTTTTTGTAACTACCCATTACTTCCTGATGTCAAGTTTTAGACCTTAGGCAGTT GGCACTAAGTCTGGTCCAAATGAATAATATAGTTTATAGTTCACATGCTGCAAACTACTAAACCT AGATTGGTGAGTGAGACCACAACTAAATTATAATAATAATTGACAAAGGTTTTTTTTCCTAATTT AACTTGGAATACTTCTAGTTTTTCAGTGGTGTATATTTGGATGCATCAATATCAATAGCAATAAG TAATAACAATAAAAGATTGCTTGATTGATGGCATTGCATATATGGGTATGGTATTGCCAATAAGA TGTTTATTTTAACTTCATTCCATTCTTGTATATGTGGAGCTTCATGGTATTCAGATTGAATGGTG TTTTTTGGCAATTTCAGCAGGTACACTGACAAAAGTGACATCTTTAGTTTTGGCATGATACTAGG TGTTTTGTTAACTGGTAAGGATCCTACAGATCCATTCTTTGGAGAAGCAGCCAGTGGGGGAAGTT TGGGATGTTGGCTGAGACACTTGCAGCAAGCGGGCGAGGCGCACGAAGCTCTAGATAAGAGCATG TTAGGGGAAGAAGGTGAGGAAGATGAGATGCTAATGGCGGTTAGGATTGCTGCTGCATGCCTCTC TGATATGCCTGCAGATAGGCCTTCTAGTGATGAGCTTGTTCACATGCTAACGCAACTGCACAGTT TTTGA Glyma18g51820 ATGTTTAGGAAAAGGCACACCCTTTCTTCTCTTGCAAGGGAATTGTTGGCATTTCAGCCACTTTT cDNA (SEQ ID TCTTCTCTTCTTGTTCAGCTTGCACCACAACACTATGCAGTGTCAAGGAAGGTTGAGTAAACATG NO: 45) Soybean TTTCTTCTGAGCCTCCCTCACCTTCTAGGTCAACACCATCACCACCATCTTCATCAGGATACAAG CRN-like gene GATGACCCTAGGAAGATAATTTTGAGCATGGTTTTAGGAGCAGTCACTGGACTAGTTTCTTCTGC TCTCTTTGCACTTGTGGTTCGTTGTGTTGTTCAGTATCTGAACCGCACACCAATCCTCAAGGGAC CTGTCATATTCTCCCCCAAAATTGCCCCCATGACACTCCAATCAGCTTTGGCAAAGGAAAACCAC TTGCTCGGTTCGAGTCCTAATGGGAAGTACTACAAAACTGTGCTTGACAATGGACTCACTATTGC AGTCAAAAGGCTAACACCCTTTGAGAGTAATTCCCCGGAGGCTAAGAGAAAATCAGTGAAGAGGC AGATACAAACTGAGCTTGAGCTTCTTGCAAGCCTTAGGCATAGGAACTTGATGAGTTTAAGGGCC TATGTTCGTGAGCCTGATGGGTTCTCATTGGTTTATGATTATGTTTCCACTGGGAGTCTTGCTGA TGTGTTGAGTAAAGTGAGGGAGAATGAGTTGCCCTTTGGTTGGGAAGTTAGGCTCAGGATTGCTG TTGGTGTGGTGAAGGGTCTTCAGTATCTTCATTTCACTTGTGTGCCTCAGATTCTGCACTACAAC TTGAAGCCCACAAATGTGATGTTGGATGCTGAGTTTGAACCTAGATTGGCAGATTATGGGTTGGC TAAACTTCTACCCAATTTGGATGGAGGAAGTTCTCTCTACACTCCTCCTGAATGTTTCCATAATT GCAGCAGGTACACTGACAAAAGTGACATCTTTAGTTTTGGCATGATACTAGGTGTTTTGTTAACT GGTAAGGATCCTACAGATCCATTCTTTGGAGAAGCAGCCAGTGGGGGAAGTTTGGGATGTTGGCT GAGACACTTGCAGCAAGCGGGCGAGGCGCACGAAGCTCTAGATAAGAGCATGTTAGGGGAAGAAG GTGAGGAAGATGAGATGCTAATGGCGGTTAGGATTGCTGCTGCATGCCTCTCTGATATGCCTGCA GATAGGCCTTCTAGTGATGAGCTTGTTCACATGCTAACGCAACTGCACAGTTTTTGA Glyma18g51820 MFRKRHTLSSLARELLAFQPLFLLFLFSLHHNTMQCQGRLSKHVSSEPPSPSRSTPSPPSSSGYK protein (SEQ DDPRKIILSMVLGAVTGLVSSALFALVVRCVVQYLNRTPILKGPVIFSPKIAPMTLQSALAKENH ID LLGSSPNGKYYKTVLDNGLTIAVKRLTPFESNSPEAKRKSVKRQIQTELELLASLRHRNLMSLRA NO: 46) Soybean YVREPDGFSLVYDYVSTGSLADVLSKVRENELPFGWEVRLRIAVGVVKGLQYLHFTCVPQILHYN CRN-like gene LKPTNVMLDAEFEPRLADYGLAKLLPNLDGGSSLYTPPECFHNCSRYTDKSDIFSFGMILGVLLT GKDPTDPFFGEAASGGSLGCWLRHLQQAGEAHEALDKSMLGEEGEEDEMLMAVRIAAACLSDMPA DRPSSDELVHMLTQLHSF Glyma08g28900 ATTATAAGAAAATAATGGTAATTTGATTTACTGAAAAAATTAGCGTCAATTCATCATTTCAATAT gDNA + about TACAAAATGATACTACGATTTAGTATCTTAAGTATTGCACGCAAAAGATTATTTTCATATCATTT 2.8 kb promoter TTCAATTATTTAGAGAAAAAAGTGACGTTAATATCTTAGGAAAAAATTAATATCTCTGGCATTTT and 5′UT AAGAAAATAAAGAGTTACTTATAAAATATGACCACGATTCATGAAATCTTATATTAAATATAGTC sequence (SEQ CCGATAATTCCAATTTGTATAAACTAAAAGAATACTTATAGGAAAAAAATAGTGAGGCAAATAAA ID TTAAACTTCTTTCATAAATAAAAATCAAATTATGTATTTTTACTTTTGGAAAAGTTAAATAAGAG NO: 47) Soybean AATTTCTTAAAATTGATTAGATAAGTTAATTTTAACTTGTGGGAGATTTTTATTTATTTATTTTT CRN-like gene CATTATACCTCTATTTTTTCTGAGTATTTTTTGAAAATTTTATCTAAATTTAAATTAAAAATTGT GGAGAATACTTTCAAGGAAAATGGCCTAATGGTTTAGCGTGTGTTTTAAGCATAAATTTTGGTAC CCATGTTTGATTAACTATTAATTAAAATTAATTTTAAAAGGCCAAGGTCATTTTCACACAATTCT ATTCCCTTGCACTAGACCACTTTTTAAGTATAAATTTATGACTAATGGGTCAAAGCATACAATGC CTTGTGTAAATAGTTGACTATCAACCAAAAATTTGACATTCAATAAGACACCACTGGTCTTTGAG CGACATCAATATTTTTATGAAAACGATAGTTGCACCTAATATGTGAAATTCGCAATCTGAATTAT TTATAAAACGTTGCATTTGCGTTCCAAATAAAAAACTCAACCCAACAAGGAAAAAAAAAACTGAA CTTATGTCTTGGTTTTGTTTTGTTTGGTTATTAAAAAAAGTAAATGGAACTGACTGTATCCAATT ATATGTCTGTTTTTAGATTTTGAAAGAAATAATTTTAAACCAATAAATATAGTTATGATATATAT TTAAATTAATCTCAGCTATATATTAAAATGTATATCACGGTAAAAATAATTAACTCTAGTAAATT ATCAAATGGATATTTGCTATAATAATTATTTGTAAATGCCTGATTTAGATTATAGTAATTTAAAA TCTAAGTACTTGTCATTTTTCATTTCAAAATGCCTCATGCCATAAATTAACCAAACAAACATGAA TTATATCCTTTGATAATTTATTCATACTATTATTGCTTGCACCTGTACATATATGTGTTGCTCAT TCACTCACCCCAGACTGAGTGCTAGATCCTCGGACTCGAATAAATCATTTATTATGCTTAAATAA TTCGATTTTATTTTTCCTACATCACAATAATCTAAAGAGTTAATCTCATACTCTCGATTCATCCT TAAAAATTTAATGGGTGTAATGAGAAATTAATTTTAACTACTATTATATTTTAAAAAATAAATAG TGAAAATAATGAGAAACTCTAATTAAAATTACTCTTTGAGTAACTAACTTGATTTTTCCTCGTAG AACAACCCAATCAATGTCACAACGAATTTTTCCTATTTCACGAGAGAACTTGAAAGTTAAAATTT GGTTAAAGTCGTTCCTAAACGTAGCAGAAGATAAGATAGAAAGATGGAAAAGTAAAAGCATGCAA ATATATAATTGAAATTGAAATGTAAGTAGTAGTAATTAAAAACAATTATTTGATGGAAAAAGAGA TAACTTTACTAATGACACACACCAAACATAAGATGTGTTCGCTAGAGTTGTTAACCACACTCACT CATATACAGCATATCACAAATTCCCATGCACCCTCAATTCAACGGTCCAGATGCGGTCTGATGAA ATCACACGGTCGATACGAACTGTACATTCTCCCTCTCTCTATCACGGATTTCGATGTTTCGCTTT CGGACCAAATGTGGGGCCCACATAGTACTGTGTCCTGAGTGCTGGCTACTCACAAAGGCGGGAAC CAGTTTTTGTCGCAGAGGTATGGCTCTTTGTTGTCATCGGATGAGAGAGAAAGAGTGTAGAGAGA GAAACAAAACTAAGAGACAATCACTGAATCACTCTCACTCACTCTACATGCTGTGTGCGTGACTC TGTCACTGTGTTTTGTGTTTAAGCACATTGCATTTTAGTTTCAGAGGAGTTTTTTTTTTTTTTTT TGCTGTTATTGTTATTCAAGTTTTGGTTACTACTACCGCCACATGTTCATGCCCCTTCAATTTTT GTTCAACTTTTTGACTTTCTGCTTGGTTTCCAAGGAAAAAGATTGCAACTTGTTTCTGGGTCTAG TTTGCTTTTGGTTGGGTTTGTTAGTCCCTGCTGGCACCTCGGAATAGTGGGTTTTTGTTTTTGTT TTTGTTTTTTTTCTTCTTTTGGAGGTTCAAATTCTTGTTCTGATTCGTGTGAAGGTGGAAAATTT ATGGGTGGTCACCGGAAGAGGAAAAAGATGGGATTCGTTGGAAAAAAGTAAGACTATTCGGTGAT AACATGTCTGCTTGCTTTTTTGGGACGGCTTTTTTGTTAAGATTTTGGGTTGAAAAGGTTGAGGA AGATGCTTATGCTTGCAACTTTTTTTTAAACCCATTTTAGCACCAAGTATAAAAAGTTGTTCTTG GTCTTGTTTCCAAGTGTTGAGGTAGGTGATAGGGGTTTTGAGCATGTGAGTGATTCATGCCTCTC ATTTTGGAGCTTCTGAGATTGGTTTCTGGTTGTGGCTTCGTTTGTTTGTTTGTTTGTTTGTTGTG CTTTCATGTTTAGGAAAAGGCACATCCTTTCTTCTCTTGCAAGGGAATTGTTGGCACTCCAGCCA CTTTTTCTTCTCTTCTTGTTCAGCTTGCACCACAACACTGTGCAGTGTCAAGGAAGGTTGAGTAA GCATGTTTCTTCAGAGCCTCCCTCACCTTCTAGGCCATCGTCAGCAGCACCATCTTCATCAGGAT ACAAGGATGACCCTAGGAAGATAATTTTGAGCATGGTTTTAGGAGCAGTCACTGGGCTAGTTTGT TCTGTTCTGTTTGCACTTGTGGTTCGTTGTGTTGTTCAGTATCTGAACCGCACACCAATCCTCAA GGGCCCTGTCATATTCTCCCCCAAAATTGCCTCCAAGACACTCCAATCAGCTTTGGCAAAGGAAA ACCACTTGCTTGGCTCGAGTCCTAATGGGAAGTACTATAAAACTATGCTTGACAATGGACTCACT ATTGCAGTCAAAAGGCTAACACCCTTTGAGAGCAATTCCCCGGAGGCCAAGAGGAAATCAGTGAA GAGGCAGATACAAACTGAGCTTGAACTTCTTGCAAGCCTTAGGAATAGGAACCTGATGAGTTTGA GAGCCTATGTTCGTGAGCCTGATGGATTCTCATTGGTTTATGATTATGCGTCCACTGGGAGTCTT GCTGATGTGTTGAATAGAGTGAGGGAGAATGAGTTGCCCTTTGGTTGGGAAGTTAGGCTCAGGAT TGCTGTTGGTGTGGTGAAGGGTCTTCAGTATCTTCACTTCACTTGTGTGCCTCAGATTCTGCACT ACAACTTGAAGCCCACTAATGTGATGTTGGATGCTGAGTTTGAACCTAGATTAGCAGATTATGGC TTGGCTAAACTTCTGCCTAACTTGGATAGAGGAAGTTCTCTCTACACCCCTCCTGAATGTTTCCA CAATTGCAGGTAAGACAAATCAATTGCTTTCAATCATACTCACTCACTAGTGTTTTGAACTTGGT TTGTTTCTGTTTTTTCACTTTTTACACCAAATGGGTAACTAGTTGGTTGATATTGGGCACTTGCT TGATTCGTTACCTTTTTAAAAGCTCCACTCCTCATTGGTTTTTTCTCCTTCTTTGGAGTACCTTA ATCAAAGACTCTTAGTGTGAAACGTGATTATTGTTCTGTATTGTCATGGTGTCATTTGCTATTGT TTAATAATTAAGACTTTGCAAAACTAATGTTTTTGTAACTACCCATTACTTGTATAGTTCACATG CTGCAAACTACTAAACCTAGATTGGTGATTGAGACCCCAATTAAAAATTATAATAATAATTTACT AAGGTTTTTCTTTTCCAATTTAACTTATTTCTAGTTTTTCATTGTTGTGTATATCTCTGGATACA TCAATCTTAATAGTAATAACTTAAAAATAAGTAATAACAATAAAAAGATTGCTTGATTGATGCAT TTCATATATGGGTATGGTATTGCCAATAAGATGTTAATTTTAACTTCATTCCATTCTTGTATGTG AAACTTCATGGTATTTAGATTGGATGGTGTTTTTTGCAATTTCAGCAGGTACACCGACAAAAGTG ATATCTTCAGTTTTGGCATCATACTAGGTGTTTTATTAACCAGTAAGGACCCTACAGATCCATTC TTTGGAGAAGCAGCCAGTGGGGGAAGTTTGGGATGTTGGTTGAGACACTTGCAGCAAGCCGGTGA GTCACGTGAAGCTCTAGATAAGAGCATGTTAGGAGAAGAAGGTGAGGAAGATGAGATGCTAATGG CTGTTAGGATTGCTGCTGCATGCCTTTCTGATATGCCTGCAGATAGGCCTTCTAGTGATGAGCTT GTTCACATGCTAACGCAACTGCACAGTTTTTGAAACAAACCTTGATTCTTCAGTTCCTAGATATT TTTTTCTTTCTCTTATCCCCTCTTTCTGTAATAAGATGATAGGGGAATTTGGTTAGTGCCCATGA TTCTGGTGTAATTGATTGTTTTGGTGTAATTGATTGTTTTGCATGATCTTGGTTTTCATGGTGTG GTTTCTAATATTCCATTTTCTCTTTCTCTATTCTATTTCCTTTTTCTTTTGGCTGATTTTGCAGG TTGTGGTGGGTTTAGGTCACACTATTATATTTTGTTTGTAAATGACTAGTCATGTTAACAAGAGT TTTCTTTTCTTGCT Glyma08g28900 ATGTTTAGGAAAAGGCACATCCTTTCTTCTCTTGCAAGGGAATTGTTGGCACTCCAGCCACTTTT cDNA (SEQ ID TCTTCTCTTCTTGTTCAGCTTGCACCACAACACTGTGCAGTGTCAAGGAAGGTTGAGTAAGCATG NO: 48) Soybean TTTCTTCAGAGCCTCCCTCACCTTCTAGGCCATCGTCAGCAGCACCATCTTCATCAGGATACAAG CRN-like gene GATGACCCTAGGAAGATAATTTTGAGCATGGTTTTAGGAGCAGTCACTGGGCTAGTTTGTTCTGT TCTGTTTGCACTTGTGGTTCGTTGTGTTGTTCAGTATCTGAACCGCACACCAATCCTCAAGGGCC CTGTCATATTCTCCCCCAAAATTGCCTCCAAGACACTCCAATCAGCTTTGGCAAAGGAAAACCAC TTGCTTGGCTCGAGTCCTAATGGGAAGTACTATAAAACTATGCTTGACAATGGACTCACTATTGC AGTCAAAAGGCTAACACCCTTTGAGAGCAATTCCCCGGAGGCCAAGAGGAAATCAGTGAAGAGGC AGATACAAACTGAGCTTGAACTTCTTGCAAGCCTTAGGAATAGGAACCTGATGAGTTTGAGAGCC TATGTTCGTGAGCCTGATGGATTCTCATTGGTTTATGATTATGCGTCCACTGGGAGTCTTGCTGA TGTGTTGAATAGAGTGAGGGAGAATGAGTTGCCCTTTGGTTGGGAAGTTAGGCTCAGGATTGCTG TTGGTGTGGTGAAGGGTCTTCAGTATCTTCACTTCACTTGTGTGCCTCAGATTCTGCACTACAAC TTGAAGCCCACTAATGTGATGTTGGATGCTGAGTTTGAACCTAGATTAGCAGATTATGGCTTGGC TAAACTTCTGCCTAACTTGGATAGAGGAAGTTCTCTCTACACCCCTCCTGAATGTTTCCACAATT GCAGCAGGTACACCGACAAAAGTGATATCTTCAGTTTTGGCATCATACTAGGTGTTTTATTAACC AGTAAGGACCCTACAGATCCATTCTTTGGAGAAGCAGCCAGTGGGGGAAGTTTGGGATGTTGGTT GAGACACTTGCAGCAAGCCGGTGAGTCACGTGAAGCTCTAGATAAGAGCATGTTAGGAGAAGAAG GTGAGGAAGATGAGATGCTAATGGCTGTTAGGATTGCTGCTGCATGCCTTTCTGATATGCCTGCA GATAGGCCTTCTAGTGATGAGCTTGTTCACATGCTAACGCAACTGCACAGTTTTTGA Glyma08g28900 MFRKRHILSSLARELLALQPLFLLFLFSLHHNTVQCQGRLSKHVSSEPPSPSRPSSAAPSSSGYK protein (SEQ DDPRKIILSMVLGAVTGLVCSVLFALVVRCVVQYLNRTPILKGPVIFSPKIASKTLQSALAKENH ID LLGSSPNGKYYKTMLDNGLTIAVKRLTPFESNSPEAKRKSVKRQIQTELELLASLRNRNLMSLRA NO: 49) Soybean YVREPDGFSLVYDYASTGSLADVLNRVRENELPFGWEVRLRIAVGVVKGLQYLHFTCVPQILHYN CRN-like gene LKPTNVMLDAEFEPRLADYGLAKLLPNLDRGSSLYTPPECFHNCSRYTDKSDIFSFGIILGVLLT SKDPTDPFFGEAASGGSLGCWLRHLQQAGESREALDKSMLGEEGEEDEMLMAVRIAAACLSDMPA DRPSSDELVHMLTQLHSF Glyma18g47610 CAAATGGGTATGCTCCCTTCAGGGGACTCCCCAATCGCCCTAATCGCAGACTCCACCGTCTCACT gDNA + about CTCGTGAAACTCCGCCAGCTCCGGCTTCCCCACCGTCAGATCGCCCACCACGTGGTACACGAACA 4 kb of CCGACGCCATCGGAATCCAAAAGGGTATCCGGAACCACAATCAAAATCGATTTTTGTTCTGCTTT promoter and TTGTATCCTTAAAAAAAAAACCGAAAACAGAAAGAAAAAAAAAAGTTTGCTTTTTTTGCTTTGTC 5′UT sequence GGGTGAGAGCTATAAGAGGGTATGGAGGAAGATGAGGAGAAGATCGAGGGCGGTGATGGGAGGGC (SEQ ID GGTGGAGGATCACGGCAGAGAAAGAGTTAGCCATTGCCATGGAGGGAGAACGAAAAGGTTAAGGC NO: 50) Soybean CCATTCAATTGAATCAGATCAGAGAGAGAGAGGGCGTAGCTTTTGGGGAAGATATGATATGTAGA CLV2-like GATTTGGATAAGGTACGTCCTTTCGGGGACAGCAAGAGATGCAACGACAGAAGAAGATGGATCAG sequence CGACGCTTGATGCGGTTGGGACCTGAGAATGAATGGGACACCAGACACACACTAAAAGGAGGTTC AATTTATCAAATAAAAAAGAGAAAGGCACAGGGGATGTGTCATGTGTCATGTGTCATGTGTCATG TGTATGGTGAGCTGCATCATATAGAGAATCTTTTCACCTTAATTAATTTGTTTAGTTTAATACGT TTTTCTTTTCTTGTCATACTCATCTTTGATTTCAATTCTATAGACCTATATATAAGTTAATTTAT TTAATAAGAGAGGATAAACAAAGAATGAAAATAGGTAAATGAGAAAAAAGGAGAAATAAATTAAA AACAATGCTTGTTTGAATTTAAAGAAACGGAAGAAAAATAAGAAAAATAGATTACTAATATAAAA TATCCTTTATATTACATAATTTTTTTCATATAACATAGTACATACGGACAAAACTTAGATACATT ATTTTGGGTGTTATTTTTTTATTAGAGTTAAAGTTTCATTTCAATGATATATATATAAGTTTTAA ATGTAAAACTTTATTATGCAAATTACTCAAATAAAACTCCAATTTTCATTAGAGAATAATACAAA CCGTGTAACGACTACAAGTTTATCTTAAATTTCCAATCTTTGAAATTATGTTATTTGTCTCCCTT TCTTAAAAATATAAAATTGATTTAGTGATAAAGAAAAAAGAGGAGAAGGGATAAGTTTTAAATAT AAATTCTTCAGGTTATAGTTCAATAGGTCACCTTTAATTAATGACGTTAATTAACAGATTAATAA TGACTTCAGAAGCAGTGTCTATGAAGTTTATGCGAGATCACCAATGATATATGTAGTTAATAGCA ACAAGTTGAGGAAAGAGGTTTGGATGAATGTGTGGCTGTTTAATGTTGGGTGGTGGTGTGGTGGC TATGACTACGAGGTTGGTGTTGGAAAATGTTGTCAATTCAATTGGGATTCGGTTTGCAAAGTTGT GATAACTTTGAGTTGAATGATGGAATATTGAAATTTTCTAGGCTTAGTTAGGAATGATTGCTACA TGTAACAGTGATACCACAACAACAGGGATGAGGGATTGTTGGGGTTTACTTTTAAAAAATGAATG AATTGAATTACAATGTAAAAGTATACATATAAAACACTATTCTTGCTTCTTAAAAAAAACGTGAG ACAGAGAGAAAGTGAAGATGATAAGATTATAGCGCACGCGTTGGAGCGTGCATGAGTTTACTAGG TCTTGTACCATGCAAAAAAATTTAGGACCCTTAGATATAACAACAAGACAAGAAGATCTTTAAGA GTGTAACATATGGATAACATACTGTATACCAACTTTTCTTTTTAATAGTATTTCTTCTCTCTGGT TATAACATCATTTTAACTAATCTATGTCTGTTAAAAAAATATTAATTTAATTAATTATATTAAAT ATATCAATTATTTATATTTTTTATTTTTCTATCCACTTAATTTTTTATTAATGTTTTAAAAAAAT AATTAAGAATAAAATAATTAATGTATTAAAAATTAAAAAAATCTTATAAATCAAGACAAATAAAT TTATGAAAAACATCATATAATTAGTATGGGATTATGGGATGGAGTAGTATTTAACTTGTGGCTTT TGAAAATTACACCATATTTTCTCTCTCTCTTGACAAAATGAATGCAACTTAAAAACGTGGGATCA TTCTTCCTCCTGAGTCCAGAATGTTCGACCCCATTCGTACTCTGATCTATGTGTGTTTGTGGTAT ATCTCCGTTGTCACTTCACCATTCTAGCTTCATCAGAGAAAGTAATATATATATTTGTAAACCAA TTATATATATATTTGAGAGGATTTTAATTCTTACTAAAATTGTAAACCAATTAGAAATCATTTTT CTGTAATTTTTGTGATCTGAAATTTTCTGTTCGGGTTGGAAATGACACAAAATCGTTGGGTCTTT AAATGGGTTGCAACCGGATGAGAATGACCCAACTCAAGGTAGGGGATGACCAAAGCATAGCCTTT TAATGGGTAATGTTAAACATGATATAAATTTATAACAAATTATTTTTATGGTGTAGTGGTTAACT CTTTCATTAATAATAATATAGCTGGTTGTTGGTTCCATCCCACAATAAGTCAGTTTAGCTTTTTA TCTTCTAAAGATTTCCTGTTTTCATTTATTTTGGTTTTTTTAAAAAATAAACAATTTCGCCTTGG AATCGAACTCACGATATAGTGATTAGTTATAAAAAAATAATTATAAATTATTTGGTAATTTTTTT CTTACATTCACTCTTGTTTTGAATACTCTTCTCTTTGTGAAGTTATGAACTTTGTTCTCTTACCA CAAATATGATACATCTTCTTATGTTTTTTAATTTTAGATTATATTTGATAAAACTAACCAAAAAG ATGAAAAATATAGTCTGTTTAAAATATTTAAGATCTAAGCTTAACTCGTTACATGTGATAGACTT TATTTGTAGATTATACTTGATTTATTTGAAAGTTTAGCTTAACCTATTAGTTTATTTAAAGACCT ATTTCATATGAAAGTTTTTATATAAGTCTATTTTTTTATATTGGACAATAAATTTATAAATCGTT GAGAAAATTCCATGTAAACAAACTATAATCTATAAAAAAAAAAAATTTCTTTATTCAAAGCACAA GATAGGTGAAAATAGATGAACTAAGTTTTATAAGTGAAATTTAACATGTCATTATGATGTAAGTT TATCAACTTCAAGATAACTTAGTTAAAAATATAATTTTGTAATAAGTCCTCTAATTAAAACATAA ATTTCGCACTCAATAATTTTTTTTTAATCGTGGATCAACACTCATAATATTTTAAAAAAGTAAAT AATGTATTATTTTGATACATTACAATAATTTTAATATTACAAAATATTATAATTTATATTTATTT AAATAGGTTGATCTATTAGGTTTAAAACACTTTTTAAATAACTTAAAACCTAATTTTTTAATCAA ATAGACTTTTATTAAAACTTAGATATGATTTATTTTTATTTTTTTAAAAAAAACTAACCTGACTT GAGTTTGATATAAATTAGGTGTCAGTTTGTTTAAATTTATTTATTAAAATAAATGTTTATTTTAA TAAAATAAGTAATTTTATATTTGTTTAGTATATTTGTGTAAATTCTTTTTCCTTAAAAAATATTT TTTTCTTTTTAAAAAAAATACTTATTTTAAAATTATTTTTTTTAAAAAGAGAAACTTGAAAAAGG ATAAAGTGTAATGCAGTATAGAGAGAAAGAGGAGGAAGCAAAGCAAACCAAGCACAACACAACAA AGCCACTTTATTTTTTTGATCTAACCTAAACCCTCTTTTTCCCCTGTTGCTCTCTCACTTTATCA GCGTGATACAACCAACCCAAGACCAATGTGGAAGATCTTGTTCCTCTTTCCCTTCTCTTATGTCC ATTTCATCATGTTTTCATTCTAATCTCCAAAATCCATGCCCACCCAGTTCCTCTTTTGCTTCAAA CTCCTCTCCCCCTTCCTAAAAATTGCACCTTTACTCTCATGGTGATGGGACACACCACACCCCTC ACACTTCTCTGTGTGATTCTTCTTTTTGCAACTCCTTCTCACTCAATTGATGTTCACCCACAAGA CAGAATCTCACTTTCAATGTTCAGGTCATCTCTGCCAAACCCCAACCAGAGTTTGCCCAGCTGGG TGGGCTCCAACTGCACTTCATGGAGTGGAATCACCTGTGACAACAGAACTGGGAGGGTGCTTTCC ATCAACCTAACCAGTATGAACCTTTCAGGCAAAATCCACCCCAGTTTGTGCTACCTTTCATATCT GAACAAGTTGGGGTTGTCCCACAACAACTTCACATCCCCTCTTCCTGAATGTTTTGGCAACTTGC TTAACCTAAGAGCCATTGATCTCAGCCACAACAGGCTTCATGGGGGAATACCAGACTCTTTCATG AGGCTTAGGCACCTCACTGAGCTTGTTTTGAGTGGGAACCCTGATTTGGGGGGTCCACTGCCTGC TTGGATTGGTAACTTCTCTGCAAATCTGGAAAGGTTACATCTTGGTTTCTGTTCATTCAGTGGTG GCATACCGGAGAGCTTGCTTTACCTGAAGTCCCTCAAGTATTTGGACCTTGAGAACAACCTCTTG TCTGGTAACTTGGTCAATTTTCAACAGCCTTTGGTTTTGCTCAATCTTGCTTCCAATCAGTTTGC TGGTACTTTGCCTTGCTTTGCAGCTTCAGTTCAGTCTCTAACTGTGTTGAATTTATCTAACAATT CTATTGTGGGGGGACTACCTGCTTGTATTGCTTCTTTTCAAGCTTTGACTCATTTGAACCTGTCA GGGAACCACTTGAAGTATAGAATATATCCTAGGCTTGTGTTCTCGGAGAAACTTCTTGTTTTGGA CTTGAGTAATAATGCTTTGTCTGGTCCTATTCCTTGTAAAATTGCTGAGACAACTGAGAAACTTG GCCTTGTTCTTCTTGACCTTTCTCACAATCAGTTCTCTGGTGAAATTCCTGTGAAAATCACTGAG TTGAAAAGCTTGCAGGCCTTGTTTCTCTCTCACAATCTTCTCTCTGGAGAAATTCCTGCTAGAAT TGGAAATTTGACTTATCTGCAGGTCATTGATCTCTCACACAACTCTTTGTCTGGAACCATTCCAT TCAGTATTGTTGGGTGCTTTCAGCTGTATGCTCTAATACTTACTAACAACAATCTTTCTGGTGTA ATTCAACCGGAGTTTGATGCGTTGGATATCTTGAGGATTCTGGATATAAGCAACAACAGGTTTTC CGGGGCTATCCCACTCACTCTGGCTGGATGCAAATCTCTGGAGATTGTAGATTTTAGTTCCAATG AGCTTTCTGGATCCTTGAATGATGCAATAACCAAATGGACAAACCTCAGGTATTTGTCTCTTGCT CAGAACAAGTTCAGTGGAAATCTGCCTAGTTGGTTGTTCACATTTAACGCAATAGAAATGATGGA TTTCTCGCATAACAAGTTTACTGGCTTCATACCTGATATTAATTTTAAGGGTAGCTTAATATTTA ACACCAGGAATGTCACTGTTAAAGAGCCATTGGTTGCAGCAAGAAAGGTTCAACTGAGAGTTTCG GCGGTTGTTTCTGATAGCAATCAGCTCAGTTTCACTTATGATCTTTCCTCAATGGTTGGAATTGA TCTATCCAGCAATTCGCTTCATGGGGAAATTCCAAGGGGCTTATTTGGTCTAGCTGGCCTAGAAT ATCTGAACTTGTCATGCAACTTTCTTTACGGACAGCTTCCGGGGTTGCAGAAAATGCATAGTTTG AAAGCCTTGGATTTGTCACATAATTCCTTGTCTGGACATATCCCAGGAAACATTTCTAGCCTTCA AGATCTGTCCATTTTGAATCTTTCCTACAACTGTTTTTCTGGATATGTTCCCCAGAAGCAAGGGT ATGGGAGATTTCCCGGTGCATTTGCTGGAAATCCAGATCTGTGCATGGAAACTTCCAGTGGAGTA TGTGATGATGGAAGGACTCAATCTGCGCAAGGAAGTTCTTTCAGTGAAGATAGGATGGATGGCCC AATTTCTGTGGGGATTTTCTTTATCAGTGCCTTTGTTAGTTTTGATTTTGGTGTTGTGGTTCTCT TCTGTTCTGCCCGGGCAAGAAATTACATTCTCCAAACAAAAGTTTGATTTGATGCTTGTGACAGT TACAAATCTCCTGTAAATTCCATTTTGTAATTTGGTACCTGTGTTCTCAGTTTCAAGTAAAACAT ACACTTATGTGACTAGGAATACTATCCGGCCATCAACTTCACAAGTGTTTTCTTGTGATTCCTGA TCAAGTGTCTCAGATTTACAGGATCAAAATGCCATGACATGAGTAACACAAGGTTTAAAGAACAC TCAACACTGGCTTTATCTATCTGAGTGAAGACTAGCCTGGCATCATTCAGCCAAGAAAAGAATGG ATGATTATGATGAAAATTTGATCCGAGTAAAGACGAGTCCCTCATCATTCTGATGGTTGTTCTCT TTTGCTGGAATTTGGTTGCATCAAGTTTAGAATGCATCATCACATGTATTATTCATAATCAGTGG TGGGCGAAGGGTCAGTAGGGAACATGTCTGATATCTGGTCTAGTTATGGTGAAATTTTGATCTTG GGCATCAAATTGCAGATTTGCAAGCATGTTTACGTGAAGAGAACTTGTATAATTCTTGATTAACC TAGTTCTTTCTTGAGGTGGGGAACCAAGTTTTCCCTGTAAGTGGGGAGTAGGTTCTCATAAGTCT AAGATTTGTATTTGTATTACTATCTTCACACCTTCATCATAGTGCTGTGATTTTAAATGATATTC TCACGAAACCTTTTCATTGACAACAGAAAAGAGGTTAATTGA Glyma18g47610 ATGCCCACCCAGTTCCTCTTTTGCTTCAAACTCCTCTCCCCCTTCCTAAAAATTGCACCTTTACT cDNA (SEQ ID CTCATGGTCATCTCTGCCAAACCCCAACCAGAGTTTGCCCAGCTGGGTGGGCTCCAACTGCACTT NO: 51) Soybean CATGGAGTGGAATCACCTGTGACAACAGAACTGGGAGGGTGCTTTCCATCAACCTAACCAGTATG CLV2-like AACCTTTCAGGCAAAATCCACCCCAGTTTGTGCTACCTTTCATATCTGAACAAGTTGGGGTTGTC sequence CCACAACAACTTCACATCCCCTCTTCCTGAATGTTTTGGCAACTTGCTTAACCTAAGAGCCATTG ATCTCAGCCACAACAGGCTTCATGGGGGAATACCAGACTCTTTCATGAGGCTTAGGCACCTCACT GAGCTTGTTTTGAGTGGGAACCCTGATTTGGGGGGTCCACTGCCTGCTTGGATTGGTAACTTCTC TGCAAATCTGGAAAGGTTACATCTTGGTTTCTGTTCATTCAGTGGTGGCATACCGGAGAGCTTGC TTTACCTGAAGTCCCTCAAGTATTTGGACCTTGAGAACAACCTCTTGTCTGGTAACTTGGTCAAT TTTCAACAGCCTTTGGTTTTGCTCAATCTTGCTTCCAATCAGTTTGCTGGTACTTTGCCTTGCTT TGCAGCTTCAGTTCAGTCTCTAACTGTGTTGAATTTATCTAACAATTCTATTGTGGGGGGACTAC CTGCTTGTATTGCTTCTTTTCAAGCTTTGACTCATTTGAACCTGTCAGGGAACCACTTGAAGTAT AGAATATATCCTAGGCTTGTGTTCTCGGAGAAACTTCTTGTTTTGGACTTGAGTAATAATGCTTT GTCTGGTCCTATTCCTTGTAAAATTGCTGAGACAACTGAGAAACTTGGCCTTGTTCTTCTTGACC TTTCTCACAATCAGTTCTCTGGTGAAATTCCTGTGAAAATCACTGAGTTGAAAAGCTTGCAGGCC TTGTTTCTCTCTCACAATCTTCTCTCTGGAGAAATTCCTGCTAGAATTGGAAATTTGACTTATCT GCAGGTCATTGATCTCTCACACAACTCTTTGTCTGGAACCATTCCATTCAGTATTGTTGGGTGCT TTCAGCTGTATGCTCTAATACTTACTAACAACAATCTTTCTGGTGTAATTCAACCGGAGTTTGAT GCGTTGGATATCTTGAGGATTCTGGATATAAGCAACAACAGGTTTTCCGGGGCTATCCCACTCAC TCTGGCTGGATGCAAATCTCTGGAGATTGTAGATTTTAGTTCCAATGAGCTTTCTGGATCCTTGA ATGATGCAATAACCAAATGGACAAACCTCAGGTATTTGTCTCTTGCTCAGAACAAGTTCAGTGGA AATCTGCCTAGTTGGTTGTTCACATTTAACGCAATAGAAATGATGGATTTCTCGCATAACAAGTT TACTGGCTTCATACCTGATATTAATTTTAAGGGTAGCTTAATATTTAACACCAGGAATGTCACTG TTAAAGAGCCATTGGTTGCAGCAAGAAAGGTTCAACTGAGAGTTTCGGCGGTTGTTTCTGATAGC AATCAGCTCAGTTTCACTTATGATCTTTCCTCAATGGTTGGAATTGATCTATCCAGCAATTCGCT TCATGGGGAAATTCCAAGGGGCTTATTTGGTCTAGCTGGCCTAGAATATCTGAACTTGTCATGCA ACTTTCTTTACGGACAGCTTCCGGGGTTGCAGAAAATGCATAGTTTGAAAGCCTTGGATTTGTCA CATAATTCCTTGTCTGGACATATCCCAGGAAACATTTCTAGCCTTCAAGATCTGTCCATTTTGAA TCTTTCCTACAACTGTTTTTCTGGATATGTTCCCCAGAAGCAAGGGTATGGGAGATTTCCCGGTG CATTTGCTGGAAATCCAGATCTGTGCATGGAAACTTCCAGTGGAGTATGTGATGATGGAAGGACT CAATCTGCGCAAGGAAGTTCTTTCAGTGAAGATAGGATGGATGGCCCAATTTCTGTGGGGATTTT CTTTATCAGTGCCTTTGTTAGTTTTGATTTTGGTGTTGTGGTTCTCTTCTGTTCTGCCCGGGCAA GAAATTACATTCTCCAAACAAAAGTTTGA Glyma18g47610 MPTQFLFCFKLLSPFLKIAPLLSWSSLPNPNQSLPSWVGSNCTSWSGITCDNRTGRVLSINLTSM protein (SEQ NLSGKIHPSLCYLSYLNKLGLSHNNFTSPLPECFGNLLNLRAIDLSHNRLHGGIPDSFMRLRHLT ID ELVLSGNPDLGGPLPAWIGNFSANLERLHLGFCSFSGGIPESLLYLKSLKYLDLENNLLSGNLVN NO: 52) Soybean FQQPLVLLNLASNQFAGTLPCFAASVQSLTVLNLSNNSIVGGLPACIASFQALTHLNLSGNHLKY CLV2-like RIYPRLVFSEKLLVLDLSNNALSGPIPCKIAETTEKLGLVLLDLSHNQFSGEIPVKITELKSLQA sequence LFLSHNLLSGEIPARIGNLTYLQVIDLSHNSLSGTIPFSIVGCFQLYALILTNNNLSGVIQPEFD ALDILRILDISNNRFSGAIPLTLAGCKSLEIVDFSSNELSGSLNDAITKWTNLRYLSLAQNKFSG NLPSWLFTFNAIEMMDFSHNKFTGFIPDINFKGSLIFNTRNVTVKEPLVAARKVQLRVSAVVSDS NQLSFTYDLSSMVGIDLSSNSLHGEIPRGLFGLAGLEYLNLSCNFLYGQLPGLQKMHSLKALDLS HNSLSGHIPGNISSLQDLSILNLSYNCFSGYVPQKQGYGRFPGAFAGNPDLCMETSSGVCDDGRT QSAQGSSFSEDRMDGPISVGIFFISAFVSFDFGVVVLFCSARARNYILQTKV

Having illustrated and described the principles of the present invention, it should be apparent to persons skilled in the art that the invention can be modified in arrangement and detail without departing from such principles. Although the materials and methods of this invention have been described in terms of various embodiments and illustrative examples, it will be apparent to those of skill in the art that variations can be applied to the materials and methods described herein without departing from the concept, spirit and scope of the invention. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims. 

What is claimed is:
 1. A method for inhibiting plant parasitic nematode damage to a potato plant, said method comprising: (1) reducing the expression of an endogenous CLAVATA2-like (CLV2-like) gene by introducing into said potato plant one or more non-natural mutations in said CLV2-like gene, or by expressing in said potato plant RNAi to silence said CLV2-like gene, wherein said CLV2-like gene is set forth in SEQ ID NO: 11, and (2) growing said potato plant comprising said one or more non-natural mutations or expressing said RNAi, thereby inhibiting plant parasitic nematode damage to said potato plant.
 2. The method of claim 1, further comprising the step of harvesting a product of said potato plant.
 3. The method of claim 2, wherein said product is a leaf, stem, flower, seed, root, or tuber.
 4. The method of claim 2, wherein the yield and/or quality of said product is increased relative to a control plant not comprising said one or more non-natural mutations or expressing said RNAi.
 5. The method of claim 1, wherein said plant parasitic nematode is a cyst nematode.
 6. The method of claim 5, wherein said cyst nematode is a Globodera spp.
 7. The method of claim 1, wherein said plant parasitic nematode is G.rostochiensis or G.pallida.
 8. A genetically modified nematode resistant potato plant produced by the method of claim 1, wherein said potato plant comprises said one or more non-natural mutations or expresses said RNAi.
 9. A method for inhibiting plant parasitic nematode damage to a soybean plant, said method comprising: (1) reducing the expression of an endogenous CLAVATA2-like (CLV2-like) gene by introducing into said soybean plant one or more non-natural mutations in said CLV2-like gene, or by expressing in said soybean plant RNAi to silence said CLV2-like gene, wherein said CLV2-like gene is set forth in SEQ ID NO: 35 or SEQ ID NO: 50; and (2) growing said soybean plant comprising said one or more non-natural mutations or expressing said RNAi, thereby inhibiting plant parasitic nematode damage to said soybean plant.
 10. The method of claim 9, further comprising the step of harvesting a product of said soybean plant.
 11. The method of claim 10, wherein said product is a leaf, stem, flower, seed, root, or tuber.
 12. The method of claim 10, wherein the yield and/or quality of said product is increased relative to a control plant not comprising said one or more non-natural mutations or expressing said RNAi.
 13. The method of claim 9, wherein said plant parasitic nematode is a cyst nematode.
 14. The method of claim 13, wherein said cyst nematode is a Heterodera spp.
 15. The method of claim 9, wherein said plant parasitic nematode is H.glycines.
 16. A genetically modified nematode resistant soybean plant produced by the method of claim 9, wherein said soybean plant comprises said one or more non-natural mutations or expresses said RNAi. 